{"abstract":[{"text":"We have examined factors that determine the strength and dynamics of GABAergic synapses between interneurons [dentate gyrus basket cells (BCs)] and principal neurons [dentate gyrus granule cells (GCs)] using paired recordings in rat hippocampal slices at 34°C. Unitary IPSCs recorded from BC–GC pairs in high intracellular Cl− concentration showed a fast rise and a biexponential decay, with mean time constants of 2 and 9 msec. The mean quantal conductance change, determined directly at reduced extracellular Ca2+/Mg2+concentration ratios, was 1.7 nS. Quantal release at the BC–GC synapse occurred with short delay and was highly synchronized. Analysis of IPSC peak amplitudes and numbers of failures by multiple probability compound binomial analysis indicated that synaptic transmission at the BC–GC synapse involves three to seven release sites, each of which releases transmitter with high probability (∼0.5 in 2 mMCa2+/1 mM Mg2+). Unitary BC–GC IPSCs showed paired-pulse depression (PPD); maximal depression, measured for 10 msec intervals, was 37%, and recovery from depression occurred with a time constant of 2 sec. Paired-pulse depression was mainly presynaptic in origin but appeared to be independent of previous release. Synaptic transmission at the BC–GC synapse showed frequency-dependent depression, with half-maximal decrease at 5 Hz after a series of 1000 presynaptic action potentials. The relative stability of transmission at the BC–GC synapse is consistent with a model in which an activity-dependent gating mechanism reduces release probability and thereby prevents depletion of the releasable pool of synaptic vesicles. Thus several mechanisms converge on the generation of powerful and sustained transmission at interneuron–principal neuron synapses in hippocampal circuits.","lang":"eng"}],"volume":20,"date_created":"2018-12-11T12:03:36Z","publication_status":"published","language":[{"iso":"eng"}],"publication":"Journal of Neuroscience","oa":1,"_id":"3489","scopus_import":"1","pmid":1,"day":"01","publisher":"Society for Neuroscience","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","article_processing_charge":"No","oa_version":"Published Version","date_updated":"2023-05-03T08:18:39Z","publist_id":"2898","external_id":{"pmid":["10908596"]},"status":"public","author":[{"full_name":"Kraushaar, Udo","last_name":"Kraushaar","first_name":"Udo"},{"orcid":"0000-0001-5001-4804","first_name":"Peter M","last_name":"Jonas","full_name":"Jonas, Peter M","id":"353C1B58-F248-11E8-B48F-1D18A9856A87"}],"issue":"15","acknowledgement":"This work was supported by grants from the Deutsche Forschungsgemeinschaft (SFB 505/C5) and the Human Frontiers Science Program Organization (RG0017/1998-B) to P.J. Novartis generously provided CGP55845A. We thank Drs. J. Bischofberger, F. A. Edwards, J. R. P. Geiger, M. V. Jones, M. Martina, and A. Roth for critically reading this manuscript. We also thank A. Blomenkamp for technical assistance.","article_type":"original","doi":"10.1523/JNEUROSCI.20-15-05594.2000","type":"journal_article","page":"5594 - 5607","intvolume":"        20","citation":{"chicago":"Kraushaar, Udo, and Peter M Jonas. “Efficacy and Stability of Quantal GABA Release at a Hippocampal Interneuron-Principal Neuron Synapse.” <i>Journal of Neuroscience</i>. Society for Neuroscience, 2000. <a href=\"https://doi.org/10.1523/JNEUROSCI.20-15-05594.2000\">https://doi.org/10.1523/JNEUROSCI.20-15-05594.2000</a>.","ista":"Kraushaar U, Jonas PM. 2000. Efficacy and stability of quantal GABA release at a hippocampal interneuron-principal neuron synapse. Journal of Neuroscience. 20(15), 5594–5607.","apa":"Kraushaar, U., &#38; Jonas, P. M. (2000). Efficacy and stability of quantal GABA release at a hippocampal interneuron-principal neuron synapse. <i>Journal of Neuroscience</i>. Society for Neuroscience. <a href=\"https://doi.org/10.1523/JNEUROSCI.20-15-05594.2000\">https://doi.org/10.1523/JNEUROSCI.20-15-05594.2000</a>","short":"U. Kraushaar, P.M. Jonas, Journal of Neuroscience 20 (2000) 5594–5607.","mla":"Kraushaar, Udo, and Peter M. Jonas. “Efficacy and Stability of Quantal GABA Release at a Hippocampal Interneuron-Principal Neuron Synapse.” <i>Journal of Neuroscience</i>, vol. 20, no. 15, Society for Neuroscience, 2000, pp. 5594–607, doi:<a href=\"https://doi.org/10.1523/JNEUROSCI.20-15-05594.2000\">10.1523/JNEUROSCI.20-15-05594.2000</a>.","ieee":"U. Kraushaar and P. M. Jonas, “Efficacy and stability of quantal GABA release at a hippocampal interneuron-principal neuron synapse,” <i>Journal of Neuroscience</i>, vol. 20, no. 15. Society for Neuroscience, pp. 5594–5607, 2000.","ama":"Kraushaar U, Jonas PM. Efficacy and stability of quantal GABA release at a hippocampal interneuron-principal neuron synapse. <i>Journal of Neuroscience</i>. 2000;20(15):5594-5607. doi:<a href=\"https://doi.org/10.1523/JNEUROSCI.20-15-05594.2000\">10.1523/JNEUROSCI.20-15-05594.2000</a>"},"date_published":"2000-08-01T00:00:00Z","extern":"1","quality_controlled":"1","title":"Efficacy and stability of quantal GABA release at a hippocampal interneuron-principal neuron synapse","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6772523/","open_access":"1"}],"publication_identifier":{"issn":["0270-6474"]},"month":"08","year":"2000"}