Action potential propagation into the presynaptic dendrites of rat mitral cells

J. Bischofberger, P.M. Jonas, Journal of Physiology 504 (1997) 359–365.

Download
No fulltext has been uploaded. References only!

Journal Article | Published
Author
;
Abstract
1. Dendritic patch-clamp recordings were obtained from mitral cells in rat olfactory bulb slices, up to 350 μm from the soma. Simultaneous dendritic and somatic whole-cell recordings indicated that action potentials (APs) evoked by somatic or dendritic current injection were initiated near the soma. Both the large amplitude (100.7 ± 1.1 mV) and the short duration (1.38 ± 0.07 ms) of the AP were maintained as the AP propagated back into the primary mitral cell dendrites. 2. Outside-out patches isolated from mitral cell dendrites contained voltage-gated Na+ channels (peak conductance density, 90 pS μm-2 at -10 mV). When an AP was used as a somatic voltage-clamp command in the presence of 1 μM tetrodotoxin (TTX), the amplitude of the dendritic potential was attenuated to 48 ± 14 mV. This shows that dendritic Na+ channels support the active back-propagation of APs. 3. Dendritic patches contained voltage-gated K+ channels with high density (conductance density, 513 pS μm-2 at 30 mV. Dendritic K+ currents were reduced to 35% by 1 mM external tetraethylammonium chloride (TEACl). When an AP was used as a somatic voltage clamp command in the presence of TEACl, the dendritic potential was markedly prolonged. This indicates that dendritic K+ channels mediate the fast repolarization of dendritic APs. 4. We conclude that voltage gated Na+ and K+ channels support dendritic APs with large amplitudes and short durations that may trigger fast transmitter release at dendrodendritic synapses in the olfactory bulb.
Publishing Year
Date Published
1997-10-15
Journal Title
Journal of Physiology
Volume
504
Issue
Pt 2
Page
359 - 365
IST-REx-ID

Cite this

Bischofberger J, Jonas PM. Action potential propagation into the presynaptic dendrites of rat mitral cells. Journal of Physiology. 1997;504(Pt 2):359-365. doi:10.1111/j.1469-7793.1997.359be.x
Bischofberger, J., & Jonas, P. M. (1997). Action potential propagation into the presynaptic dendrites of rat mitral cells. Journal of Physiology, 504(Pt 2), 359–365. https://doi.org/10.1111/j.1469-7793.1997.359be.x
Bischofberger, Joseph, and Peter M Jonas. “Action Potential Propagation into the Presynaptic Dendrites of Rat Mitral Cells.” Journal of Physiology 504, no. Pt 2 (1997): 359–65. https://doi.org/10.1111/j.1469-7793.1997.359be.x.
J. Bischofberger and P. M. Jonas, “Action potential propagation into the presynaptic dendrites of rat mitral cells,” Journal of Physiology, vol. 504, no. Pt 2, pp. 359–365, 1997.
Bischofberger J, Jonas PM. 1997. Action potential propagation into the presynaptic dendrites of rat mitral cells. Journal of Physiology. 504(Pt 2), 359–365.
Bischofberger, Joseph, and Peter M. Jonas. “Action Potential Propagation into the Presynaptic Dendrites of Rat Mitral Cells.” Journal of Physiology, vol. 504, no. Pt 2, Wiley-Blackwell, 1997, pp. 359–65, doi:10.1111/j.1469-7793.1997.359be.x.

Export

Marked Publications

Open Data IST Research Explorer

Search this title in

Google Scholar