--- _id: '3482' abstract: - lang: eng text: AMPA- and NMDA-type glutamate receptors (AMPARs and NMDARs) mediate excitatory synoptic transmission in the basal ganglia and may contribute to excitotoxic injury. We investigated the functional properties of AMPARs and NMDARs expressed by six main types of basal ganglia neurons in acute rat brain slices (principal neurons and cholinergic interneurons of striatum, GABAergic and dopaminergic neurons of substantia nigra, globus pallidus neurons, and subthalamic nucleus neurons) using fast application of glutamate to nucleated and outside-out membrane patches, AMPARs in different types of basal ganglia neurons were functionally distinct. Those expressed in striatal principal neurons exhibited the slowest gating (desensitization time constant τ = 11.5 msec, 1 mM glutamate, 22°C), whereas those in striatal cholinergic interneurons showed the fastest gating (desensitization time constant τ = 3.6 msec). The lowest Ca2+ permeability of AMPARs was observed in nigral dopaminergic neurons (P(CA)/P(NA) = 0.10), whereas the highest Ca2+ permeability was found in subthalamic nucleus neurons (P(Ca)/P(Na) = 1.17). NMDARs of different types of basal ganglia neurons were less variable in their functional properties; those expressed in nigral dopaminergic neurons exhibited the slowest gating (deactivation time constant of predominant fast component τ1 150 msec, 100 μM glutamate), and those of globus pallidus neurons showed the fastest gating (τ1 = 67 msec). The Mg2+ block of NMDARs was similar; the average chord conductance ratio g(+60mv)/g(+40mV) was 0.18-0.22 in 100 μM external Mg2+. Hence, AMPARs expressed in different types of basal ganglia neurons are markedly diverse, whereas NMDARs are less variable in functional properties that are relevant for excitatory synoptic transmission and neuronal vulnerability. acknowledgement: "This work was supported by Deutsche Forschungsgemeinschaft Grant BE1859 to T.B. and SFB505/C5 to P.J. We thank Mrs. B. Plessow-Freudenberg for help with the immunocytochemistry, Dr. M. Ha¨usser for advice concerning the \r\n reparation of midbrain slices, and Drs. J. Bischofberger, G. B. Landwehrmeyer, and M. Martina for critically reading this manuscript." article_processing_charge: No article_type: original author: - first_name: Thomas full_name: Götz, Thomas last_name: Götz - first_name: Udo full_name: Kraushaar, Udo last_name: Kraushaar - first_name: Jörg full_name: Geiger, Jörg last_name: Geiger - first_name: Joachim full_name: Lubke, Joachim last_name: Lubke - first_name: Thomas full_name: Berger, Thomas last_name: Berger - first_name: Peter M full_name: Jonas, Peter M id: 353C1B58-F248-11E8-B48F-1D18A9856A87 last_name: Jonas orcid: 0000-0001-5001-4804 citation: ama: Götz T, Kraushaar U, Geiger J, Lubke J, Berger T, Jonas PM. Functional properties of AMPA and NMDA receptors expressed in identified types of basal ganglia neurons. Journal of Neuroscience. 1997;17(1):204-215. doi:10.1523/JNEUROSCI.17-01-00204.1997 apa: Götz, T., Kraushaar, U., Geiger, J., Lubke, J., Berger, T., & Jonas, P. M. (1997). Functional properties of AMPA and NMDA receptors expressed in identified types of basal ganglia neurons. Journal of Neuroscience. Society for Neuroscience. https://doi.org/10.1523/JNEUROSCI.17-01-00204.1997 chicago: Götz, Thomas, Udo Kraushaar, Jörg Geiger, Joachim Lubke, Thomas Berger, and Peter M Jonas. “Functional Properties of AMPA and NMDA Receptors Expressed in Identified Types of Basal Ganglia Neurons.” Journal of Neuroscience. Society for Neuroscience, 1997. https://doi.org/10.1523/JNEUROSCI.17-01-00204.1997. ieee: T. Götz, U. Kraushaar, J. Geiger, J. Lubke, T. Berger, and P. M. Jonas, “Functional properties of AMPA and NMDA receptors expressed in identified types of basal ganglia neurons,” Journal of Neuroscience, vol. 17, no. 1. Society for Neuroscience, pp. 204–215, 1997. ista: Götz T, Kraushaar U, Geiger J, Lubke J, Berger T, Jonas PM. 1997. Functional properties of AMPA and NMDA receptors expressed in identified types of basal ganglia neurons. Journal of Neuroscience. 17(1), 204–215. mla: Götz, Thomas, et al. “Functional Properties of AMPA and NMDA Receptors Expressed in Identified Types of Basal Ganglia Neurons.” Journal of Neuroscience, vol. 17, no. 1, Society for Neuroscience, 1997, pp. 204–15, doi:10.1523/JNEUROSCI.17-01-00204.1997. short: T. Götz, U. Kraushaar, J. Geiger, J. Lubke, T. Berger, P.M. Jonas, Journal of Neuroscience 17 (1997) 204–215. date_created: 2018-12-11T12:03:34Z date_published: 1997-01-01T00:00:00Z date_updated: 2022-08-22T08:48:45Z day: '01' doi: 10.1523/JNEUROSCI.17-01-00204.1997 extern: '1' external_id: pmid: - '8987749' intvolume: ' 17' issue: '1' language: - iso: eng main_file_link: - open_access: '1' url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6793708/ month: '01' oa: 1 oa_version: Published Version page: 204 - 215 pmid: 1 publication: Journal of Neuroscience publication_identifier: issn: - 0270-6474 publication_status: published publisher: Society for Neuroscience publist_id: '2905' quality_controlled: '1' scopus_import: '1' status: public title: Functional properties of AMPA and NMDA receptors expressed in identified types of basal ganglia neurons type: journal_article user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17 volume: 17 year: '1997' ...