Differences in Ca(2+) permeability of AMPA-type glutamate receptor channels in neocortical neurons caused by differential GluR-B subunit expression
Jonas PM, Racca C, Sakmann B, Seeburg P, Monyer H. 1994. Differences in Ca(2+) permeability of AMPA-type glutamate receptor channels in neocortical neurons caused by differential GluR-B subunit expression. Neuron. 12(6), 1281–1289.
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Journal Article
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| English
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Author
Jonas, Peter MISTA 
;
Racca, Claudia;
Sakmann, Bert;
Seeburg, Peter;
Monyer, Hannah
;
Racca, Claudia;
Sakmann, Bert;
Seeburg, Peter;
Monyer, HannahAbstract
Fast excitatory synaptic transmission in the CNS is mediated by AMPA-type glutamate receptor (GluR) channels. Heterologous expression suggested that the Ca2+ permeability of these receptors critically depends on the subunit composition. Using patch-clamp techniques in brain slices, we found that the Ca2+ permeability of native AMPA-type GluRs was markedly higher in nonpyramidal (P(Ca)/P(K) ≃ 0.63) than in pyramidal (P(Ca)/P(K) ≃ 0.05) neurons of rat neocortex. Analysis of mRNA in single cells indicated that the relative abundance of GluR-B-specific mRNA was significantly lower in nonpyramidal (GluR-B/GluR-non-B ≃ 0.3) than in pyramidal (GluR-B/GluR-non-B ≃ 3) cells. This suggests that differences in relative abundance of GluR-B- specific mRNA generate functional diversity of AMPA-type GluRs in neurons with respect to Ca2+ permeability.
Publishing Year
Date Published
1994-06-01
Journal Title
Neuron
Acknowledgement
We thank Drs. B. Lambolez and J. Rossier for helping to establish the method of single-cell PCR, Dr. M. Frotscher for help with cell identification, Dr. N. Spruston for critically reading the manuscript, and M. Kaiser and U. Keller for technical assistance. This work was supported in part by BMFT grant BCT 364 AZ 3211 7291 (P. H. S.) and by DFG grant SFB-317/B14 (P. J.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 USC Section 1734 solely to indicate this fact.
Volume
12
Issue
6
Page
1281 - 1289
ISSN
IST-REx-ID
Cite this
Jonas PM, Racca C, Sakmann B, Seeburg P, Monyer H. Differences in Ca(2+) permeability of AMPA-type glutamate receptor channels in neocortical neurons caused by differential GluR-B subunit expression. Neuron. 1994;12(6):1281-1289. doi:10.1016/0896-6273(94)90444-8
Jonas, P. M., Racca, C., Sakmann, B., Seeburg, P., & Monyer, H. (1994). Differences in Ca(2+) permeability of AMPA-type glutamate receptor channels in neocortical neurons caused by differential GluR-B subunit expression. Neuron. Elsevier. https://doi.org/10.1016/0896-6273(94)90444-8
Jonas, Peter M, Claudia Racca, Bert Sakmann, Peter Seeburg, and Hannah Monyer. “Differences in Ca(2+) Permeability of AMPA-Type Glutamate Receptor Channels in Neocortical Neurons Caused by Differential GluR-B Subunit Expression.” Neuron. Elsevier, 1994. https://doi.org/10.1016/0896-6273(94)90444-8.
P. M. Jonas, C. Racca, B. Sakmann, P. Seeburg, and H. Monyer, “Differences in Ca(2+) permeability of AMPA-type glutamate receptor channels in neocortical neurons caused by differential GluR-B subunit expression,” Neuron, vol. 12, no. 6. Elsevier, pp. 1281–1289, 1994.
Jonas PM, Racca C, Sakmann B, Seeburg P, Monyer H. 1994. Differences in Ca(2+) permeability of AMPA-type glutamate receptor channels in neocortical neurons caused by differential GluR-B subunit expression. Neuron. 12(6), 1281–1289.
Jonas, Peter M., et al. “Differences in Ca(2+) Permeability of AMPA-Type Glutamate Receptor Channels in Neocortical Neurons Caused by Differential GluR-B Subunit Expression.” Neuron, vol. 12, no. 6, Elsevier, 1994, pp. 1281–89, doi:10.1016/0896-6273(94)90444-8.
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