---
_id: '3439'
abstract:
- lang: eng
text: High molecular weight DNA was extracted from the primary Neotropical malaria
vector, Anopheles darlingi from Capanema, Pará, Brazil, to create a small insert
genomic library, and then a phagemid library. Enriched sublibraries were constructed
from the phagemid library using a microsatellite oligo primed second strand synthesis
protocol. The resulting 242 760 individual clones were screened. The mean clone
size of the positive clones was 302 bp. Flanking primers were designed for each
suitable microsatellite sequence. Eight polymorphic loci were optimized and characterized.
The allele size ranges are based on 253 samples of A. darlingi from eastern Amazonian
and central Brazil.
acknowledgement: For support in Brazil we thank D. Galiza, R.N.L. Lacerda,E.P.
Santa Rosa, M.N.O. Segura, and R.T.L. de Souza. We also thankM.J. Braun for allowing work on the library construction at theLaboratory
of Molecular Systematics, Washington. Supported byNIH AI 40116 to JEC and Instituto
Evandro Chagas, Belém, Brazil.
article_processing_charge: No
article_type: original
author:
- first_name: Jan
full_name: Conn, Jan
last_name: Conn
- first_name: Jonathan P
full_name: Bollback, Jonathan P
id: 2C6FA9CC-F248-11E8-B48F-1D18A9856A87
last_name: Bollback
orcid: 0000-0002-4624-4612
- first_name: David
full_name: Onyabe, David
last_name: Onyabe
- first_name: Tessa
full_name: Robinson, Tessa
last_name: Robinson
- first_name: Richard
full_name: Wilkerson, Richard
last_name: Wilkerson
- first_name: Marinete
full_name: Povoa, Marinete
last_name: Povoa
citation:
ama: Conn J, Bollback JP, Onyabe D, Robinson T, Wilkerson R, Povoa M. Isolation
of polymorphic microsatellite markers from the malaria vector Anopheles darlingi.
Molecular Ecology Notes. 2001;1(4):223-225. doi: 10.1046/j.1471-8278.2001.00078.x
apa: Conn, J., Bollback, J. P., Onyabe, D., Robinson, T., Wilkerson, R., & Povoa,
M. (2001). Isolation of polymorphic microsatellite markers from the malaria vector
Anopheles darlingi. Molecular Ecology Notes. Wiley-Blackwell. https://doi.org/ 10.1046/j.1471-8278.2001.00078.x
chicago: Conn, Jan, Jonathan P Bollback, David Onyabe, Tessa Robinson, Richard Wilkerson,
and Marinete Povoa. “Isolation of Polymorphic Microsatellite Markers from the
Malaria Vector Anopheles Darlingi.” Molecular Ecology Notes. Wiley-Blackwell,
2001. https://doi.org/
10.1046/j.1471-8278.2001.00078.x.
ieee: J. Conn, J. P. Bollback, D. Onyabe, T. Robinson, R. Wilkerson, and M. Povoa,
“Isolation of polymorphic microsatellite markers from the malaria vector Anopheles
darlingi,” Molecular Ecology Notes, vol. 1, no. 4. Wiley-Blackwell, pp.
223–225, 2001.
ista: Conn J, Bollback JP, Onyabe D, Robinson T, Wilkerson R, Povoa M. 2001. Isolation
of polymorphic microsatellite markers from the malaria vector Anopheles darlingi.
Molecular Ecology Notes. 1(4), 223–225.
mla: Conn, Jan, et al. “Isolation of Polymorphic Microsatellite Markers from the
Malaria Vector Anopheles Darlingi.” Molecular Ecology Notes, vol. 1, no.
4, Wiley-Blackwell, 2001, pp. 223–25, doi:
10.1046/j.1471-8278.2001.00078.x.
short: J. Conn, J.P. Bollback, D. Onyabe, T. Robinson, R. Wilkerson, M. Povoa, Molecular
Ecology Notes 1 (2001) 223–225.
date_created: 2018-12-11T12:03:20Z
date_published: 2001-12-01T00:00:00Z
date_updated: 2023-05-15T13:58:49Z
day: '01'
doi: ' 10.1046/j.1471-8278.2001.00078.x'
extern: '1'
intvolume: ' 1'
issue: '4'
language:
- iso: eng
month: '12'
oa_version: None
page: 223 - 225
publication: Molecular Ecology Notes
publication_identifier:
issn:
- 1471-8278
publication_status: published
publisher: Wiley-Blackwell
publist_id: '2961'
quality_controlled: '1'
status: public
title: Isolation of polymorphic microsatellite markers from the malaria vector Anopheles
darlingi
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 1
year: '2001'
...