--- _id: '3439' abstract: - lang: eng text: High molecular weight DNA was extracted from the primary Neotropical malaria vector, Anopheles darlingi from Capanema, Pará, Brazil, to create a small insert genomic library, and then a phagemid library. Enriched sublibraries were constructed from the phagemid library using a microsatellite oligo primed second strand synthesis protocol. The resulting 242 760 individual clones were screened. The mean clone size of the positive clones was 302 bp. Flanking primers were designed for each suitable microsatellite sequence. Eight polymorphic loci were optimized and characterized. The allele size ranges are based on 253 samples of A. darlingi from eastern Amazonian and central Brazil. acknowledgement: For support in Brazil we thank D. Galiza, R.N.L. Lacerda,E.P. Santa Rosa, M.N.O. Segura, and R.T.L. de Souza. We also thankM.J. Braun for allowing work on the library construction at theLaboratory of Molecular Systematics, Washington. Supported byNIH AI 40116 to JEC and Instituto Evandro Chagas, Belém, Brazil. article_processing_charge: No article_type: original author: - first_name: Jan full_name: Conn, Jan last_name: Conn - first_name: Jonathan P full_name: Bollback, Jonathan P id: 2C6FA9CC-F248-11E8-B48F-1D18A9856A87 last_name: Bollback orcid: 0000-0002-4624-4612 - first_name: David full_name: Onyabe, David last_name: Onyabe - first_name: Tessa full_name: Robinson, Tessa last_name: Robinson - first_name: Richard full_name: Wilkerson, Richard last_name: Wilkerson - first_name: Marinete full_name: Povoa, Marinete last_name: Povoa citation: ama: Conn J, Bollback JP, Onyabe D, Robinson T, Wilkerson R, Povoa M. Isolation of polymorphic microsatellite markers from the malaria vector Anopheles darlingi. Molecular Ecology Notes. 2001;1(4):223-225. doi: 10.1046/j.1471-8278.2001.00078.x apa: Conn, J., Bollback, J. P., Onyabe, D., Robinson, T., Wilkerson, R., & Povoa, M. (2001). Isolation of polymorphic microsatellite markers from the malaria vector Anopheles darlingi. Molecular Ecology Notes. Wiley-Blackwell. https://doi.org/ 10.1046/j.1471-8278.2001.00078.x chicago: Conn, Jan, Jonathan P Bollback, David Onyabe, Tessa Robinson, Richard Wilkerson, and Marinete Povoa. “Isolation of Polymorphic Microsatellite Markers from the Malaria Vector Anopheles Darlingi.” Molecular Ecology Notes. Wiley-Blackwell, 2001. https://doi.org/ 10.1046/j.1471-8278.2001.00078.x. ieee: J. Conn, J. P. Bollback, D. Onyabe, T. Robinson, R. Wilkerson, and M. Povoa, “Isolation of polymorphic microsatellite markers from the malaria vector Anopheles darlingi,” Molecular Ecology Notes, vol. 1, no. 4. Wiley-Blackwell, pp. 223–225, 2001. ista: Conn J, Bollback JP, Onyabe D, Robinson T, Wilkerson R, Povoa M. 2001. Isolation of polymorphic microsatellite markers from the malaria vector Anopheles darlingi. Molecular Ecology Notes. 1(4), 223–225. mla: Conn, Jan, et al. “Isolation of Polymorphic Microsatellite Markers from the Malaria Vector Anopheles Darlingi.” Molecular Ecology Notes, vol. 1, no. 4, Wiley-Blackwell, 2001, pp. 223–25, doi: 10.1046/j.1471-8278.2001.00078.x. short: J. Conn, J.P. Bollback, D. Onyabe, T. Robinson, R. Wilkerson, M. Povoa, Molecular Ecology Notes 1 (2001) 223–225. date_created: 2018-12-11T12:03:20Z date_published: 2001-12-01T00:00:00Z date_updated: 2023-05-15T13:58:49Z day: '01' doi: ' 10.1046/j.1471-8278.2001.00078.x' extern: '1' intvolume: ' 1' issue: '4' language: - iso: eng month: '12' oa_version: None page: 223 - 225 publication: Molecular Ecology Notes publication_identifier: issn: - 1471-8278 publication_status: published publisher: Wiley-Blackwell publist_id: '2961' quality_controlled: '1' status: public title: Isolation of polymorphic microsatellite markers from the malaria vector Anopheles darlingi type: journal_article user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17 volume: 1 year: '2001' ...