Complex stability of single proteins explored by forced unfolding experiments

H.L. Janovjak, T. Sapra, D. Mueller, Biophysical Journal 88 (2005) 37–39.


Journal Article | Published
Author
; ;
Abstract
In the last decade atomic force microscopy has been used to measure the mechanical stability of single proteins. These force spectroscopy experiments have shown that many water-soluble and membrane proteins unfold via one or more intermediates. Recently, Li and co-workers found a linear correlation between the unfolding force of the native state and the intermediate in fibronectin, which they suggested indicated the presence of a molecular memory or multiple unfolding pathways (1). Here, we apply two independent methods in combination with Monte Carlo simulations to analyze the unfolding of α-helices E and D of bacteriorhodopsin (BR). We show that correlation analysis of unfolding forces is very sensitive to errors in force calibration of the instrument. In contrast, a comparison of relative forces provides a robust measure for the stability of unfolding intermediates. The proposed approach detects three energetically different states of α-helices E and D in trimeric BR. These states are not observed for monomeric BR and indicate that substantial information is hidden in forced unfolding experiments of single proteins.
Publishing Year
Date Published
2005-05-01
Journal Title
Biophysical Journal
Volume
88
Issue
5
Page
37 - 39
IST-REx-ID

Cite this

Janovjak HL, Sapra T, Mueller D. Complex stability of single proteins explored by forced unfolding experiments. Biophysical Journal. 2005;88(5):37-39. doi:10.1529/biophysj.105.059774
Janovjak, H. L., Sapra, T., & Mueller, D. (2005). Complex stability of single proteins explored by forced unfolding experiments. Biophysical Journal, 88(5), 37–39. https://doi.org/10.1529/biophysj.105.059774
Janovjak, Harald L, Tanuj Sapra, and Daniel Mueller. “Complex Stability of Single Proteins Explored by Forced Unfolding Experiments.” Biophysical Journal 88, no. 5 (2005): 37–39. https://doi.org/10.1529/biophysj.105.059774.
H. L. Janovjak, T. Sapra, and D. Mueller, “Complex stability of single proteins explored by forced unfolding experiments,” Biophysical Journal, vol. 88, no. 5, pp. 37–39, 2005.
Janovjak HL, Sapra T, Mueller D. 2005. Complex stability of single proteins explored by forced unfolding experiments. Biophysical Journal. 88(5), 37–39.
Janovjak, Harald L., et al. “Complex Stability of Single Proteins Explored by Forced Unfolding Experiments.” Biophysical Journal, vol. 88, no. 5, Biophysical Society, 2005, pp. 37–39, doi:10.1529/biophysj.105.059774.

Link(s) to Main File(s)
Access Level
OA Open Access

Export

Marked Publications

Open Data IST Research Explorer

Search this title in

Google Scholar