--- res: bibo_abstract: - Migrating lymphocytes acquire a polarized phenotype with a leading and a trailing edge, or uropod. Although in vitro experiments in cell lines or activated primary cell cultures have established that Rho-p160 coiled-coil kinase (ROCK)-myosin II-mediated uropod contractility is required for integrin de-adhesion on two-dimensional surfaces and nuclear propulsion through narrow pores in three-dimensional matrices, less is known about the role of these two events during the recirculation of primary, nonactivated lymphocytes. Using pharmacological antagonists of ROCK and myosin II, we report that inhibition of uropod contractility blocked integrin-independent mouse T cell migration through narrow, but not large, pores in vitro. T cell crawling on chemokine-coated endothelial cells under shear was severely impaired by ROCK inhibition, whereas transendothelial migration was only reduced through endothelial cells with high, but not low, barrier properties. Using three-dimensional thick-tissue imaging and dynamic two-photon microscopy of T cell motility in lymphoid tissue, we demonstrated a significant role for uropod contractility in intraluminal crawling and transendothelial migration through lymph node, but not bone marrow, endothelial cells. Finally, we demonstrated that ICAM-1, but not anatomical constraints or integrin-independent interactions, reduced parenchymal motility of inhibitor-treated T cells within the dense lymphoid microenvironment, thus assigning context-dependent roles for uropod contraction during lymphocyte recirculation.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Silvia foaf_name: Soriano, Silvia foaf_surname: Soriano - foaf_Person: foaf_givenName: Miroslav foaf_name: Hons, Miroslav foaf_surname: Hons orcid: 0000-0002-6625-3348 - foaf_Person: foaf_givenName: Kathrin foaf_name: Schumann, Kathrin foaf_surname: Schumann - foaf_Person: foaf_givenName: Varsha foaf_name: Kumar, Varsha foaf_surname: Kumar - foaf_Person: foaf_givenName: Timo foaf_name: Dennier, Timo foaf_surname: Dennier - foaf_Person: foaf_givenName: Ruth foaf_name: Lyck, Ruth foaf_surname: Lyck - foaf_Person: foaf_givenName: Michael K foaf_name: Sixt, Michael K foaf_surname: Sixt foaf_workInfoHomepage: http://www.librecat.org/personId=41E9FBEA-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-6620-9179 - foaf_Person: foaf_givenName: Jens foaf_name: Stein, Jens foaf_surname: Stein bibo_doi: 10.4049/jimmunol.1100935 bibo_issue: '5' bibo_volume: 187 dct_date: 2011^xs_gYear dct_isPartOf: - http://id.crossref.org/issn/0022-1767 - http://id.crossref.org/issn/1550-6606 dct_language: eng dct_publisher: American Association of Immunologists@ dct_title: In vivo analysis of uropod function during physiological T cell trafficking@ ...