--- res: bibo_abstract: - Cationic antimicrobial peptides (CAMPs) selectively target bacterial membranes by electrostatic interactions with negatively charged lipids. It turned out that for inhibition of microbial growth a high CAMP membrane concentration is required, which can be realized by the incorporation of hydrophobic groups within the peptide. Increasing hydrophobicity, however, reduces the CAMP selectivity for bacterial over eukaryotic host membranes, thereby causing the risk of detrimental side-effects. In this study we addressed how cationic amphipathic peptides—in particular a CAMP with Lysine–Leucine–Lysine repeats (termed KLK)—affect the localization and dynamics of molecules in eukaryotic membranes. We found KLK to selectively inhibit the endocytosis of a subgroup of membrane proteins and lipids by electrostatically interacting with negatively charged sialic acid moieties. Ultrastructural characterization revealed the formation of membrane invaginations representing fission or fusion intermediates, in which the sialylated proteins and lipids were immobilized. Experiments on structurally different cationic amphipathic peptides (KLK, 6-MO-LF11-322 and NK14-2) indicated a cooperation of electrostatic and hydrophobic forces that selectively arrest sialylated membrane constituents.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Julian foaf_name: Weghuber, Julian foaf_surname: Weghuber - foaf_Person: foaf_givenName: Michael foaf_name: Aichinger, Michael C. foaf_surname: Aichinger - foaf_Person: foaf_givenName: Mario foaf_name: Brameshuber, Mario foaf_surname: Brameshuber - foaf_Person: foaf_givenName: Stefan foaf_name: Stefan Wieser foaf_surname: Wieser foaf_workInfoHomepage: http://www.librecat.org/personId=355AA5A0-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-2670-2217 - foaf_Person: foaf_givenName: Verena foaf_name: Verena Ruprecht foaf_surname: Ruprecht foaf_workInfoHomepage: http://www.librecat.org/personId=4D71A03A-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0003-4088-8633 - foaf_Person: foaf_givenName: Birgit foaf_name: Plochberger, Birgit foaf_surname: Plochberger - foaf_Person: foaf_givenName: Josef foaf_name: Madl, Josef foaf_surname: Madl - foaf_Person: foaf_givenName: Andreas foaf_name: Horner, Andreas foaf_surname: Horner - foaf_Person: foaf_givenName: Siegfried foaf_name: Reipert, Siegfried foaf_surname: Reipert - foaf_Person: foaf_givenName: Karl foaf_name: Lohner, Karl foaf_surname: Lohner - foaf_Person: foaf_givenName: Tamas foaf_name: Henics, Tamas foaf_surname: Henics - foaf_Person: foaf_givenName: Gerhard foaf_name: Schuetz, Gerhard J foaf_surname: Schuetz bibo_doi: 10.1016/j.bbamem.2011.06.007 bibo_issue: '10' bibo_volume: 1808 dct_date: 2011^xs_gYear dct_publisher: Elsevier@ dct_title: Cationic amphipathic peptides accumulate sialylated proteins and lipids in the plasma membrane of eukaryotic host cells@ ...