---
_id: '3153'
abstract:
- lang: eng
text: Human immune cells have to penetrate an endothelial barrier during their beneficial
pursuit of infection and their destructive infiltration of tissues in autoimmune
diseases. This transmigration requires Rap1 GTPase to activate integrin affinity.
We define a new model system for this process by demonstrating, with live imaging
and genetics, that during embryonic development Drosophila melanogaster immune
cells penetrate an epithelial, Drosophila E-cadherin (DE-cadherin)-based tissue
barrier. A mutant in RhoL, a GTPase homologue that is specifically expressed in
haemocytes, blocks this invasive step but not other aspects of guided migration.
RhoL mediates integrin adhesion caused by Drosophila Rap1 overexpression and moves
Rap1 away from a concentration in the cytoplasm to the leading edge during invasive
migration. These findings indicate that a programmed migratory step during Drosophila
development bears striking molecular similarities to vertebrate immune cell transmigration
during inflammation, and identify RhoL as a new regulator of invasion, adhesion
and Rap1 localization. Our work establishes the utility of Drosophila for identifying
novel components of immune cell transmigration and for understanding the in vivo
interplay of immune cells with the barriers they penetrate.
author:
- first_name: Daria E
full_name: Daria Siekhaus
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
- first_name: Martin
full_name: Haesemeyer, Martin
last_name: Haesemeyer
- first_name: Olivia
full_name: Moffitt, Olivia
last_name: Moffitt
- first_name: Ruth
full_name: Lehmann, Ruth
last_name: Lehmann
citation:
ama: Siekhaus DE, Haesemeyer M, Moffitt O, Lehmann R. RhoL controls invasion and
Rap1 localization during immune cell transmigration in Drosophila. Nature Cell
Biology. 2010;12(6):605-610.
apa: Siekhaus, D. E., Haesemeyer, M., Moffitt, O., & Lehmann, R. (2010). RhoL
controls invasion and Rap1 localization during immune cell transmigration in Drosophila.
Nature Cell Biology. Nature Publishing Group.
chicago: Siekhaus, Daria E, Martin Haesemeyer, Olivia Moffitt, and Ruth Lehmann.
“RhoL Controls Invasion and Rap1 Localization during Immune Cell Transmigration
in Drosophila.” Nature Cell Biology. Nature Publishing Group, 2010.
ieee: D. E. Siekhaus, M. Haesemeyer, O. Moffitt, and R. Lehmann, “RhoL controls
invasion and Rap1 localization during immune cell transmigration in Drosophila,”
Nature Cell Biology, vol. 12, no. 6. Nature Publishing Group, pp. 605–610,
2010.
ista: Siekhaus DE, Haesemeyer M, Moffitt O, Lehmann R. 2010. RhoL controls invasion
and Rap1 localization during immune cell transmigration in Drosophila. Nature
Cell Biology. 12(6), 605–610.
mla: Siekhaus, Daria E., et al. “RhoL Controls Invasion and Rap1 Localization during
Immune Cell Transmigration in Drosophila.” Nature Cell Biology, vol. 12,
no. 6, Nature Publishing Group, 2010, pp. 605–10.
short: D.E. Siekhaus, M. Haesemeyer, O. Moffitt, R. Lehmann, Nature Cell Biology
12 (2010) 605–610.
date_created: 2018-12-11T12:01:42Z
date_published: 2010-06-01T00:00:00Z
date_updated: 2021-01-12T07:41:25Z
day: '01'
extern: 1
intvolume: ' 12'
issue: '6'
main_file_link:
- open_access: '0'
url: 10.1038/ncb2063 PubMed
month: '06'
page: 605 - 610
publication: Nature Cell Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '3542'
quality_controlled: 0
status: public
title: RhoL controls invasion and Rap1 localization during immune cell transmigration
in Drosophila
type: journal_article
volume: 12
year: '2010'
...