--- res: bibo_abstract: - Microinjection of recombinant DNA into zygotic pronuclei has been widely used for producing transgenic mice. However, with this method, the insertion site, integrity, and copy number of the transgene cannot be controlled. Here, we present an integrase-based approach to produce transgenic mice via pronuclear injection, whereby an intact single-copy transgene can be inserted into predetermined chromosomal loci with high efficiency (up to 40%), and faithfully transmitted through generations. We show that neighboring transgenic elements and bacterial DNA within the transgene cause profound silencing and expression variability of the transgenic marker. Removal of these undesirable elements leads to global high-level marker expression from transgenes driven by a ubiquitous promoter. We also obtained faithful marker expression from a tissue-specific promoter. The technique presented here will greatly facilitate murine transgenesis and precise structure/function dissection of mammalian gene function and regulation in vivo.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Bosiljka foaf_name: Tasic, Bosiljka foaf_surname: Tasic - foaf_Person: foaf_givenName: Simon foaf_name: Simon Hippenmeyer foaf_surname: Hippenmeyer foaf_workInfoHomepage: http://www.librecat.org/personId=37B36620-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0003-2279-1061 - foaf_Person: foaf_givenName: Charlene foaf_name: Wang, Charlene foaf_surname: Wang - foaf_Person: foaf_givenName: Matthew foaf_name: Gamboa, Matthew foaf_surname: Gamboa - foaf_Person: foaf_givenName: Hui foaf_name: Zong, Hui foaf_surname: Zong - foaf_Person: foaf_givenName: Yanru foaf_name: Chen-Tsai, Yanru foaf_surname: Chen Tsai - foaf_Person: foaf_givenName: Liqun foaf_name: Luo, Liqun foaf_surname: Luo bibo_doi: 10.1073/pnas.1019507108 bibo_issue: '19' bibo_volume: 108 dct_date: 2011^xs_gYear dct_publisher: National Academy of Sciences@ dct_title: Site specific integrase mediated transgenesis in mice via pronuclear injection@ ...