--- res: bibo_abstract: - Cell polarization via asymmetrical distribution of structures or molecules is essential for diverse cellular functions and development of organisms, but how polarity is developmentally controlled has been poorly understood. In plants, the asymmetrical distribution of the PIN-FORMED (PIN) proteins involved in the cellular efflux of the quintessential phytohormone auxin plays a central role in developmental patterning, morphogenesis, and differential growth. Recently we showed that auxin promotes cell interdigitation by activating the Rho family ROP GTPases in leaf epidermal pavement cells. Here we found that auxin activation of the ROP2 signaling pathway regulates the asymmetric distribution of PIN1 by inhibiting its endocytosis. ROP2 inhibits PIN1 endocytosis via the accumulation of cortical actin microfilaments induced by the ROP2 effector protein RIC4. Our findings suggest a link between the developmental auxin signal and polar PIN1 distribution via Rho-dependent cytoskeletal reorganization and reveal the conservation of a design principle for cell polarization that is based on Rho GTPase-mediated inhibition of endocytosis.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Shingo foaf_name: Nagawa, Shingo foaf_surname: Nagawa - foaf_Person: foaf_givenName: Tongda foaf_name: Xu, Tongda foaf_surname: Xu - foaf_Person: foaf_givenName: Deshu foaf_name: Lin, Deshu foaf_surname: Lin - foaf_Person: foaf_givenName: Pankaj foaf_name: Dhonukshe, Pankaj foaf_surname: Dhonukshe - foaf_Person: foaf_givenName: Xingxing foaf_name: Zhang, Xingxing foaf_surname: Zhang - foaf_Person: foaf_givenName: Jirí foaf_name: Jirí Friml foaf_surname: Friml foaf_workInfoHomepage: http://www.librecat.org/personId=4159519E-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-8302-7596 - foaf_Person: foaf_givenName: Ben foaf_name: Scheres, Ben foaf_surname: Scheres - foaf_Person: foaf_givenName: Ying foaf_name: Fu, Ying foaf_surname: Fu - foaf_Person: foaf_givenName: Zhenbiao foaf_name: Yang, Zhenbiao foaf_surname: Yang bibo_doi: 10.1371/journal.pbio.1001299 bibo_issue: '4' bibo_volume: 10 dct_date: 2012^xs_gYear dct_publisher: Public Library of Science@ dct_title: ROP GTPase-dependent actin microfilaments promote PIN1 polarization by localized inhibition of clathrin-dependent endocytosis@ ...