--- res: bibo_abstract: - Endocytosis is a crucial mechanism by which eukaryotic cells internalize extracellular and plasma membrane material, and it is required for a multitude of cellular and developmental processes in unicellular and multicellular organisms. In animals and yeast, the best characterized pathway for endocytosis depends on the function of the vesicle coat protein clathrin. Clathrinmediated endocytosis has recently been demonstrated also in plant cells, but its physiological and developmental roles remain unclear. Here, we assessed the roles of the clathrin-mediated mechanism of endocytosis in plants by genetic means. We interfered with clathrin heavy chain (CHC) function through mutants and dominant-negative approaches in Arabidopsis thaliana and established tools to manipulate clathrin function in a cell type-specific manner. The chc2 single mutants and dominant-negative CHC1 (HUB) transgenic lines were defective in bulk endocytosis as well as in internalization of prominent plasma membrane proteins. Interference with clathrin-mediated endocytosis led to defects in constitutive endocytic recycling of PIN auxin transporters and their polar distribution in embryos and roots. Consistent with this, these lines had altered auxin distribution patterns and associated auxin transport-related phenotypes, such as aberrant embryo patterning, imperfect cotyledon specification, agravitropic growth, and impaired lateral root organogenesis. Together, these data demonstrate a fundamental role for clathrin function in cell polarity, growth, patterning, and organogenesis in plants.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Saeko foaf_name: Kitakura, Saeko foaf_surname: Kitakura - foaf_Person: foaf_givenName: Steffen foaf_name: Vanneste, Steffen foaf_surname: Vanneste - foaf_Person: foaf_givenName: Stéphanie foaf_name: Robert, Stéphanie foaf_surname: Robert - foaf_Person: foaf_givenName: Christian foaf_name: Löfke, Christian foaf_surname: Löfke - foaf_Person: foaf_givenName: Thomas foaf_name: Teichmann, Thomas foaf_surname: Teichmann - foaf_Person: foaf_givenName: Hirokazu foaf_name: Tanaka, Hirokazu foaf_surname: Tanaka - foaf_Person: foaf_givenName: Jirí foaf_name: Jirí Friml foaf_surname: Friml foaf_workInfoHomepage: http://www.librecat.org/personId=4159519E-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-8302-7596 bibo_doi: 10.1105/tpc.111.083030 bibo_issue: '5' bibo_volume: 23 dct_date: 2011^xs_gYear dct_publisher: American Society of Plant Biologists@ dct_title: Clathrin mediates endocytosis and polar distribution of PIN auxin transporters in Arabidopsis@ ...