Gravity induced PIN transcytosis for polarization of auxin fluxes in gravity sensing root cells

J. Kleine Vehn, Z. Ding, A. Jones, M. Tasaka, M. Morita, J. Friml, PNAS 107 (2010) 22344–22349.

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Abstract
Auxin is an essential plant-specific regulator of patterning processes that also controls directional growth of roots and shoots. In response to gravity stimulation, the PIN3 auxin transporter polarizes to the bottomside of gravity-sensing root cells, presumably redirecting the auxin flux toward the lower side of the root and triggering gravitropic bending. By combining live-cell imaging techniques with pharmacological and genetic approaches, we demonstrate that PIN3 polarization does not require secretion of de novo synthesized proteins or protein degradation, but instead involves rapid, transient stimulation of PIN endocytosis, presumably via a clathrin-dependent pathway. Moreover, gravity-induced PIN3 polarization requires the activity of the guanine nucleotide exchange factors for ARF GTPases (ARF-GEF) GNOM-dependent polar-targeting path-ways and might involve endosome-based PIN3 translocation from one cell side to another. Our data suggest that gravity perception acts at several instances of PIN3 trafficking, ultimately leading to the polarization of PIN3, which presumably aligns auxin fluxes with gravity vector and mediates downstream root gravitropic response.
Publishing Year
Date Published
2010-12-21
Journal Title
PNAS
Volume
107
Issue
51
Page
22344 - 22349
IST-REx-ID

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Kleine Vehn J, Ding Z, Jones A, Tasaka M, Morita M, Friml J. Gravity induced PIN transcytosis for polarization of auxin fluxes in gravity sensing root cells. PNAS. 2010;107(51):22344-22349. doi:10.1073/pnas.1013145107
Kleine Vehn, J., Ding, Z., Jones, A., Tasaka, M., Morita, M., & Friml, J. (2010). Gravity induced PIN transcytosis for polarization of auxin fluxes in gravity sensing root cells. PNAS, 107(51), 22344–22349. https://doi.org/10.1073/pnas.1013145107
Kleine Vehn, Jürgen, Zhaojun Ding, Angharad Jones, Masao Tasaka, Miyo Morita, and Jirí Friml. “Gravity Induced PIN Transcytosis for Polarization of Auxin Fluxes in Gravity Sensing Root Cells.” PNAS 107, no. 51 (2010): 22344–49. https://doi.org/10.1073/pnas.1013145107.
J. Kleine Vehn, Z. Ding, A. Jones, M. Tasaka, M. Morita, and J. Friml, “Gravity induced PIN transcytosis for polarization of auxin fluxes in gravity sensing root cells,” PNAS, vol. 107, no. 51, pp. 22344–22349, 2010.
Kleine Vehn J, Ding Z, Jones A, Tasaka M, Morita M, Friml J. 2010. Gravity induced PIN transcytosis for polarization of auxin fluxes in gravity sensing root cells. PNAS. 107(51), 22344–22349.
Kleine Vehn, Jürgen, et al. “Gravity Induced PIN Transcytosis for Polarization of Auxin Fluxes in Gravity Sensing Root Cells.” PNAS, vol. 107, no. 51, National Academy of Sciences, 2010, pp. 22344–49, doi:10.1073/pnas.1013145107.

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