Sauer, Michael ; Friml, JiríIST Austria
Hennig, Lars ; Köhler, Claudia
Methods in Molecular Biology
Rapid advances in the field of plant biology, especially in plant cell biology, have created the need for methods that allow the localization of proteins in situ at subcellular resolution. Although in many cases recombinant proteins with fluorescent proteins can fulfill this task, antibody-based immunological detection of proteins is a complementary technique, which avoids the risk of inducing side effects by a fusion protein, such as misexpression, mistargeting, altered stability, or toxicity. Moreover, recombinant protein techniques are applicable only to a rather limited set of model plants. The immunolocalization protocols presented here can be used to display protein localization patterns in different tissues of various plant species. This chapter describes a whole mount immunolocalization protocol, which has been extensively used in Arabidopsis roots and some above-ground tissues, and that also works in other species. Additionally, for bulky or hard tissue types, a variation of this protocol for paraffin-embedded sections is given.
Plant Developmental Biology
253 - 263
Sauer M, Friml J. Immunolocalization of proteins in plants . In: Hennig L, Köhler C, eds. Plant Developmental Biology. Vol 655. Humana Press; 2010:253-263. doi:10.1007/978-1-60761-765-5_17
Sauer, M., & Friml, J. (2010). Immunolocalization of proteins in plants . In L. Hennig & C. Köhler (Eds.), Plant Developmental Biology (Vol. 655, pp. 253–263). Humana Press. https://doi.org/10.1007/978-1-60761-765-5_17
Sauer, Michael, and Jirí Friml. “Immunolocalization of Proteins in Plants .” In Plant Developmental Biology, edited by Lars Hennig and Claudia Köhler, 655:253–63. Humana Press, 2010. https://doi.org/10.1007/978-1-60761-765-5_17.
M. Sauer and J. Friml, “Immunolocalization of proteins in plants ,” in Plant Developmental Biology, vol. 655, L. Hennig and C. Köhler, Eds. Humana Press, 2010, pp. 253–263.
Sauer M, Friml J. 2010. Immunolocalization of proteins in plants . Plant Developmental Biology. , Methods in Molecular Biology, vol. 655. 253–263.
Sauer, Michael, and Jirí Friml. “Immunolocalization of Proteins in Plants .” Plant Developmental Biology, edited by Lars Hennig and Claudia Köhler, vol. 655, Humana Press, 2010, pp. 253–63, doi:10.1007/978-1-60761-765-5_17.