The AP 3 β adaptin mediates the biogenesis and function of lytic vacuoles in Arabidopsis

E. Feraru, T. Paciorek, M. Feraru, M. Zwiewka, R. De Groodt, R. De Rycke, J. Kleine Vehn, J. Friml, Plant Cell 22 (2010) 2812–2824.

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Abstract
Plant vacuoles are essential multifunctional organelles largely distinct from similar organelles in other eukaryotes. Embryo protein storage vacuoles and the lytic vacuoles that perform a general degradation function are the best characterized, but little is known about the biogenesis and transition between these vacuolar types. Here, we designed a fluorescent marker- based forward genetic screen in Arabidopsis thaliana and identified a protein affected trafficking2 (pat2) mutant, whose lytic vacuoles display altered morphology and accumulation of proteins. Unlike other mutants affecting the vacuole, pat2 is specifically defective in the biogenesis, identity, and function of lytic vacuoles but shows normal sorting of proteins to storage vacuoles. PAT2 encodes a putative β-subunit of adaptor protein complex 3 (AP-3) that can partially complement the corresponding yeast mutant. Manipulations of the putative AP-3 β adaptin functions suggest a plant-specific role for the evolutionarily conserved AP-3 β in mediating lytic vacuole performance and transition of storage into the lytic vacuoles independently of the main prevacuolar compartment-based trafficking route.
Publishing Year
Date Published
2010-08-01
Journal Title
Plant Cell
Volume
22
Issue
8
Page
2812 - 2824
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Feraru E, Paciorek T, Feraru M, et al. The AP 3 β adaptin mediates the biogenesis and function of lytic vacuoles in Arabidopsis. Plant Cell. 2010;22(8):2812-2824. doi:10.1105/tpc.110.075424
Feraru, E., Paciorek, T., Feraru, M., Zwiewka, M., De Groodt, R., De Rycke, R., … Friml, J. (2010). The AP 3 β adaptin mediates the biogenesis and function of lytic vacuoles in Arabidopsis. Plant Cell, 22(8), 2812–2824. https://doi.org/10.1105/tpc.110.075424
Feraru, Elena, Tomasz Paciorek, Mugurel Feraru, Marta Zwiewka, Ruth De Groodt, Riet De Rycke, Jürgen Kleine Vehn, and Jirí Friml. “The AP 3 β Adaptin Mediates the Biogenesis and Function of Lytic Vacuoles in Arabidopsis.” Plant Cell 22, no. 8 (2010): 2812–24. https://doi.org/10.1105/tpc.110.075424.
E. Feraru et al., “The AP 3 β adaptin mediates the biogenesis and function of lytic vacuoles in Arabidopsis,” Plant Cell, vol. 22, no. 8, pp. 2812–2824, 2010.
Feraru E, Paciorek T, Feraru M, Zwiewka M, De Groodt R, De Rycke R, Kleine Vehn J, Friml J. 2010. The AP 3 β adaptin mediates the biogenesis and function of lytic vacuoles in Arabidopsis. Plant Cell. 22(8), 2812–2824.
Feraru, Elena, et al. “The AP 3 β Adaptin Mediates the Biogenesis and Function of Lytic Vacuoles in Arabidopsis.” Plant Cell, vol. 22, no. 8, American Society of Plant Biologists, 2010, pp. 2812–24, doi:10.1105/tpc.110.075424.

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