{"date_updated":"2021-01-12T07:40:48Z","quality_controlled":0,"_id":"3066","extern":1,"abstract":[{"text":"In animals, the interface between organism and environment is constituted by the epithelium [1]. In plants, the exchange of nutrients and signals between root and soil is crucial for their survival, but the cellular mechanisms underlying the epithelium-like function and specific localization of proteins to the root surface have not been identified [2]. Here we analyze the mechanism of polar delivery to the root-soil interface of the proteins BOR4, ABCG37, and PEN3, which transport nutrients [2], transport plant hormones, and are required for pathogen defense [3], respectively. The simultaneous visualization of these proteins and the apical and basal cargos in a single cell demonstrates that the outermost cell side represents an additional polar domain. Delivery to this outer polar domain depends on ARF GEF [4] and actin [5-8] function but does not require known molecular components of the apical or basal targeting. The outer polar delivery is, in contrast to known basal and apical cargos [9, 10], mediated by the polar secretion. Our findings show that the outermost cell membranes of roots define an additional polar domain in plant cells along with a specific, previously uncharacterized, polar targeting mechanism that is important for defining the functional, epithelium-like root-soil interface.","lang":"eng"}],"doi":"10.1016/j.cub.2010.03.059","title":"Trafficking to the outer polar domain defines the root soil interface","intvolume":"        20","publisher":"Cell Press","day":"25","author":[{"full_name":"Łangowski, Łukasz","first_name":"Łukasz","last_name":"Łangowski"},{"full_name":"Růžička, Kamil","first_name":"Kamil","last_name":"Růžička"},{"last_name":"Naramoto","full_name":"Naramoto, Satoshi","first_name":"Satoshi"},{"last_name":"Kleine Vehn","first_name":"Jürgen","full_name":"Kleine-Vehn, Jürgen"},{"full_name":"Jirí Friml","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596"}],"publication_status":"published","date_published":"2010-05-25T00:00:00Z","issue":"10","date_created":"2018-12-11T12:01:10Z","page":"904 - 908","citation":{"chicago":"Łangowski, Łukasz, Kamil Růžička, Satoshi Naramoto, Jürgen Kleine Vehn, and Jiří Friml. “Trafficking to the Outer Polar Domain Defines the Root Soil Interface.” <i>Current Biology</i>. Cell Press, 2010. <a href=\"https://doi.org/10.1016/j.cub.2010.03.059\">https://doi.org/10.1016/j.cub.2010.03.059</a>.","short":"Ł. Łangowski, K. Růžička, S. Naramoto, J. Kleine Vehn, J. Friml, Current Biology 20 (2010) 904–908.","apa":"Łangowski, Ł., Růžička, K., Naramoto, S., Kleine Vehn, J., &#38; Friml, J. (2010). Trafficking to the outer polar domain defines the root soil interface. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2010.03.059\">https://doi.org/10.1016/j.cub.2010.03.059</a>","ista":"Łangowski Ł, Růžička K, Naramoto S, Kleine Vehn J, Friml J. 2010. Trafficking to the outer polar domain defines the root soil interface. Current Biology. 20(10), 904–908.","ama":"Łangowski Ł, Růžička K, Naramoto S, Kleine Vehn J, Friml J. Trafficking to the outer polar domain defines the root soil interface. <i>Current Biology</i>. 2010;20(10):904-908. doi:<a href=\"https://doi.org/10.1016/j.cub.2010.03.059\">10.1016/j.cub.2010.03.059</a>","mla":"Łangowski, Łukasz, et al. “Trafficking to the Outer Polar Domain Defines the Root Soil Interface.” <i>Current Biology</i>, vol. 20, no. 10, Cell Press, 2010, pp. 904–08, doi:<a href=\"https://doi.org/10.1016/j.cub.2010.03.059\">10.1016/j.cub.2010.03.059</a>.","ieee":"Ł. Łangowski, K. Růžička, S. Naramoto, J. Kleine Vehn, and J. Friml, “Trafficking to the outer polar domain defines the root soil interface,” <i>Current Biology</i>, vol. 20, no. 10. Cell Press, pp. 904–908, 2010."},"volume":20,"year":"2010","publication":"Current Biology","month":"05","status":"public","type":"journal_article","publist_id":"3634"}