PIN phosphorylation is sufficient to mediate PIN polarity and direct auxin transport

J. Zhang, T. Nodzyński, A. Pěnčík, J. Rolčík, J. Friml, PNAS 107 (2010) 918–922.

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Abstract
The plant hormone auxin plays a crucial role in regulating plant development and plant architecture. The directional auxin distribution within tissues depends on PIN transporters that are polarly localized on the plasmamembrane. The PINpolarity and the resulting auxin flow directionality aremediated by the antagonistic actions of PINOID kinase and protein phosphatase 2A. However, the contributionof the PINphosphorylationto the polar PINsortingis still unclear. Here, we identified an evolutionarily conserved phosphorylation site within the central hydrophilic loop of PIN proteins that is important for the apical and basal polar PIN localizations. Inactivation of the phosphorylation site in PIN1(Ala) resulted in a predominantly basal targeting and increased the auxinflowto the root tip. In contrast, the outcome of the phosphomimic PIN1(Asp) manipulation was a constitutive, PINOID-independent apical targeting of PIN1 and an increased auxin flow in the opposite direction. Furthermore, the PIN1(Asp) functionally replaced PIN2 in its endogenous expression domain, revealing that the phosphorylation-dependent polarity regulation contributes to functional diversification within the PIN family. Our data suggest that PINOID-independent PIN phosphorylation at one single site is adequate to change the PIN polarity and, consequently, to redirect auxin fluxes between cells and provide the conceptual possibility and means to manipulate auxin-dependent plant development and architecture.
Publishing Year
Date Published
2010-01-12
Journal Title
PNAS
Volume
107
Issue
2
Page
918 - 922
IST-REx-ID

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Zhang J, Nodzyński T, Pěnčík A, Rolčík J, Friml J. PIN phosphorylation is sufficient to mediate PIN polarity and direct auxin transport. PNAS. 2010;107(2):918-922. doi:10.1073/pnas.0909460107
Zhang, J., Nodzyński, T., Pěnčík, A., Rolčík, J., & Friml, J. (2010). PIN phosphorylation is sufficient to mediate PIN polarity and direct auxin transport. PNAS, 107(2), 918–922. https://doi.org/10.1073/pnas.0909460107
Zhang, Jing, Thomasz Nodzyński, Aleš Pěnčík, Jakub Rolčík, and Jirí Friml. “PIN Phosphorylation Is Sufficient to Mediate PIN Polarity and Direct Auxin Transport.” PNAS 107, no. 2 (2010): 918–22. https://doi.org/10.1073/pnas.0909460107.
J. Zhang, T. Nodzyński, A. Pěnčík, J. Rolčík, and J. Friml, “PIN phosphorylation is sufficient to mediate PIN polarity and direct auxin transport,” PNAS, vol. 107, no. 2, pp. 918–922, 2010.
Zhang J, Nodzyński T, Pěnčík A, Rolčík J, Friml J. 2010. PIN phosphorylation is sufficient to mediate PIN polarity and direct auxin transport. PNAS. 107(2), 918–922.
Zhang, Jing, et al. “PIN Phosphorylation Is Sufficient to Mediate PIN Polarity and Direct Auxin Transport.” PNAS, vol. 107, no. 2, National Academy of Sciences, 2010, pp. 918–22, doi:10.1073/pnas.0909460107.

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