--- res: bibo_abstract: - The polar, sub-cellular localization of PIN auxin efflux carriers determines the direction of intercellular auxin flow, thus defining the spatial aspect of auxin signalling. Dynamic, transcytosis-like relocalizations of PIN proteins occur in response to external and internal signals, integrating these signals into changes in auxin distribution. Here, we examine the cellular and molecular mechanisms of polar PIN delivery and transcytosis. The mechanisms of the ARF-GEF-dependent polar targeting and transcytosis are well conserved and show little variations among diverse Arabidopsis ecotypes consistent with their fundamental importance in regulating plant development. At the cellular level, we refine previous findings on the role of the actin cytoskeleton in apical and basal PIN targeting, and identify a previously unknown role for microtubules, specifically in basal targeting. PIN protein delivery to different sides of the cell is mediated by ARF-dependent trafficking with a previously unknown complex level of distinct ARF-GEF vesicle trafficking regulators. Our data suggest that alternative recruitment of PIN proteins by these distinct pathways can account for cell type- and cargo-specific aspects of polar targeting, as well as for polarity changes in response to different signals. The resulting dynamic PIN positioning to different sides of cells defines a three-dimensional pattern of auxin fluxes within plant tissues.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Jürgen foaf_name: Kleine-Vehn, Jürgen foaf_surname: Kleine Vehn - foaf_Person: foaf_givenName: Łukasz foaf_name: Łangowski, Łukasz foaf_surname: Łangowski - foaf_Person: foaf_givenName: Justyna foaf_name: Wiśniewska, Justyna foaf_surname: Wiśniewska - foaf_Person: foaf_givenName: Pankaj foaf_name: Dhonukshe, Pankaj foaf_surname: Dhonukshe - foaf_Person: foaf_givenName: Philip foaf_name: Brewer, Philip B foaf_surname: Brewer - foaf_Person: foaf_givenName: Jirí foaf_name: Jirí Friml foaf_surname: Friml foaf_workInfoHomepage: http://www.librecat.org/personId=4159519E-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-8302-7596 bibo_doi: 10.1093/mp/ssn062 bibo_issue: '6' bibo_volume: 1 dct_date: 2008^xs_gYear dct_publisher: Oxford University Press@ dct_title: Cellular and molecular requirements for polar PIN targeting and transcytosis in plants@ ...