--- _id: '3035' abstract: - lang: eng text: Embryogenesis of Arabidopsis thaliana follows a nearly invariant cell division pattern and provides an ideal system for studies of early plant development. However, experimental manipulation with embryogenesis is difficult, as the embryo develops deeply inside maternal tissues. Here, we present a method to culture zygotic Arabidopsis embryos in vitro. It enables culturing for prolonged periods of time from the first developmental stages on. The technique omits excision of the embryo by culturing the entire ovule, which facilitates the manual procedure. It allows pharmacological manipulation of embryo development and does not interfere with standard techniques for localizing gene expression and protein localization in the cultivated embryos. alternative_title: - Methods In Molecular Biology author: - first_name: Michael full_name: Sauer, Michael last_name: Sauer - first_name: Jirí full_name: Jirí Friml id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: 'Sauer M, Friml J. In vitro culture of Arabidopsis embryos . In: Suárez M, Bozhkov P, eds. Plant Embryogenesis. Vol 427. Humana Press; 2008:71-76. doi:10.1007/978-1-59745-273-1_5' apa: Sauer, M., & Friml, J. (2008). In vitro culture of Arabidopsis embryos . In M. Suárez & P. Bozhkov (Eds.), Plant Embryogenesis (Vol. 427, pp. 71–76). Humana Press. https://doi.org/10.1007/978-1-59745-273-1_5 chicago: Sauer, Michael, and Jiří Friml. “In Vitro Culture of Arabidopsis Embryos .” In Plant Embryogenesis, edited by María Suárez and Peter Bozhkov, 427:71–76. Humana Press, 2008. https://doi.org/10.1007/978-1-59745-273-1_5. ieee: M. Sauer and J. Friml, “In vitro culture of Arabidopsis embryos ,” in Plant Embryogenesis, vol. 427, M. Suárez and P. Bozhkov, Eds. Humana Press, 2008, pp. 71–76. ista: 'Sauer M, Friml J. 2008.In vitro culture of Arabidopsis embryos . In: Plant Embryogenesis. Methods In Molecular Biology, vol. 427, 71–76.' mla: Sauer, Michael, and Jiří Friml. “In Vitro Culture of Arabidopsis Embryos .” Plant Embryogenesis, edited by María Suárez and Peter Bozhkov, vol. 427, Humana Press, 2008, pp. 71–76, doi:10.1007/978-1-59745-273-1_5. short: M. Sauer, J. Friml, in:, M. Suárez, P. Bozhkov (Eds.), Plant Embryogenesis, Humana Press, 2008, pp. 71–76. date_created: 2018-12-11T12:00:59Z date_published: 2008-03-07T00:00:00Z date_updated: 2021-01-12T07:40:35Z day: '07' doi: 10.1007/978-1-59745-273-1_5 editor: - first_name: María full_name: Suárez, María F last_name: Suárez - first_name: Peter full_name: Bozhkov, Peter V last_name: Bozhkov extern: 1 intvolume: ' 427' month: '03' page: 71 - 76 publication: Plant Embryogenesis publication_status: published publisher: Humana Press publist_id: '3667' quality_controlled: 0 status: public title: 'In vitro culture of Arabidopsis embryos ' type: book_chapter volume: 427 year: '2008' ...