---
_id: '3035'
abstract:
- lang: eng
text: Embryogenesis of Arabidopsis thaliana follows a nearly invariant cell division
pattern and provides an ideal system for studies of early plant development. However,
experimental manipulation with embryogenesis is difficult, as the embryo develops
deeply inside maternal tissues. Here, we present a method to culture zygotic Arabidopsis
embryos in vitro. It enables culturing for prolonged periods of time from the
first developmental stages on. The technique omits excision of the embryo by culturing
the entire ovule, which facilitates the manual procedure. It allows pharmacological
manipulation of embryo development and does not interfere with standard techniques
for localizing gene expression and protein localization in the cultivated embryos.
alternative_title:
- Methods In Molecular Biology
author:
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Sauer M, Friml J. In vitro culture of Arabidopsis embryos . In: Suárez M,
Bozhkov P, eds. Plant Embryogenesis. Vol 427. Humana Press; 2008:71-76.
doi:10.1007/978-1-59745-273-1_5'
apa: Sauer, M., & Friml, J. (2008). In vitro culture of Arabidopsis embryos
. In M. Suárez & P. Bozhkov (Eds.), Plant Embryogenesis (Vol. 427,
pp. 71–76). Humana Press. https://doi.org/10.1007/978-1-59745-273-1_5
chicago: Sauer, Michael, and Jiří Friml. “In Vitro Culture of Arabidopsis Embryos
.” In Plant Embryogenesis, edited by María Suárez and Peter Bozhkov, 427:71–76.
Humana Press, 2008. https://doi.org/10.1007/978-1-59745-273-1_5.
ieee: M. Sauer and J. Friml, “In vitro culture of Arabidopsis embryos ,” in Plant
Embryogenesis, vol. 427, M. Suárez and P. Bozhkov, Eds. Humana Press, 2008,
pp. 71–76.
ista: 'Sauer M, Friml J. 2008.In vitro culture of Arabidopsis embryos . In: Plant
Embryogenesis. Methods In Molecular Biology, vol. 427, 71–76.'
mla: Sauer, Michael, and Jiří Friml. “In Vitro Culture of Arabidopsis Embryos .”
Plant Embryogenesis, edited by María Suárez and Peter Bozhkov, vol. 427,
Humana Press, 2008, pp. 71–76, doi:10.1007/978-1-59745-273-1_5.
short: M. Sauer, J. Friml, in:, M. Suárez, P. Bozhkov (Eds.), Plant Embryogenesis,
Humana Press, 2008, pp. 71–76.
date_created: 2018-12-11T12:00:59Z
date_published: 2008-03-07T00:00:00Z
date_updated: 2021-01-12T07:40:35Z
day: '07'
doi: 10.1007/978-1-59745-273-1_5
editor:
- first_name: María
full_name: Suárez, María F
last_name: Suárez
- first_name: Peter
full_name: Bozhkov, Peter V
last_name: Bozhkov
extern: 1
intvolume: ' 427'
month: '03'
page: 71 - 76
publication: Plant Embryogenesis
publication_status: published
publisher: Humana Press
publist_id: '3667'
quality_controlled: 0
status: public
title: 'In vitro culture of Arabidopsis embryos '
type: book_chapter
volume: 427
year: '2008'
...