In vitro culture of Arabidopsis embryos

M. Sauer, J. Friml, in:, M. Suárez, P. Bozhkov (Eds.), Plant Embryogenesis, Humana Press, 2008, pp. 71–76.

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Series Title
Methods In Molecular Biology
Abstract
Embryogenesis of Arabidopsis thaliana follows a nearly invariant cell division pattern and provides an ideal system for studies of early plant development. However, experimental manipulation with embryogenesis is difficult, as the embryo develops deeply inside maternal tissues. Here, we present a method to culture zygotic Arabidopsis embryos in vitro. It enables culturing for prolonged periods of time from the first developmental stages on. The technique omits excision of the embryo by culturing the entire ovule, which facilitates the manual procedure. It allows pharmacological manipulation of embryo development and does not interfere with standard techniques for localizing gene expression and protein localization in the cultivated embryos.
Publishing Year
Date Published
2008-03-07
Book Title
Plant Embryogenesis
Volume
427
Page
71 - 76
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Sauer M, Friml J. In vitro culture of Arabidopsis embryos . In: Suárez M, Bozhkov P, eds. Plant Embryogenesis. Vol 427. Humana Press; 2008:71-76. doi:10.1007/978-1-59745-273-1_5
Sauer, M., & Friml, J. (2008). In vitro culture of Arabidopsis embryos . In M. Suárez & P. Bozhkov (Eds.), Plant Embryogenesis (Vol. 427, pp. 71–76). Humana Press. https://doi.org/10.1007/978-1-59745-273-1_5
Sauer, Michael, and Jirí Friml. “In Vitro Culture of Arabidopsis Embryos .” In Plant Embryogenesis, edited by María Suárez and Peter Bozhkov, 427:71–76. Humana Press, 2008. https://doi.org/10.1007/978-1-59745-273-1_5.
M. Sauer and J. Friml, “In vitro culture of Arabidopsis embryos ,” in Plant Embryogenesis, vol. 427, M. Suárez and P. Bozhkov, Eds. Humana Press, 2008, pp. 71–76.
Sauer M, Friml J. 2008. In vitro culture of Arabidopsis embryos . Plant Embryogenesis. , Methods In Molecular Biology, vol. 427. 71–76.
Sauer, Michael, and Jirí Friml. “In Vitro Culture of Arabidopsis Embryos .” Plant Embryogenesis, edited by María Suárez and Peter Bozhkov, vol. 427, Humana Press, 2008, pp. 71–76, doi:10.1007/978-1-59745-273-1_5.

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