---
_id: '3031'
abstract:
- lang: eng
text: Many aspects of plant development, including patterning and tropisms, are
largely dependent on the asymmetric distribution of the plant signaling molecule
auxin. Auxin transport inhibitors (ATIs), which interfere with directional auxin
transport, have been essential tools in formulating this concept. However, despite
the use of ATIs in plant research for many decades, the mechanism of ATI action
has remained largely elusive. Using real-time live-cell microscopy, we show here
that prominent ATIs such as 2,3,5-triiodobenzoic acid (TIBA) and 2-(1-pyrenoyl)
benzoic acid (PBA) inhibit vesicle trafficking in plant, yeast, and mammalian
cells. Effects on micropinocytosis, rab5-labeled endosomal motility at the periphery
of HeLa cells and on fibroblast mobility indicate that ATIs influence actin cytoskeleton.
Visualization of actin cytoskeleton dynamics in plants, yeast, and mammalian cells
show that ATIs stabilize actin. Conversely, stabilizing actin by chemical or genetic
means interferes with endocytosis, vesicle motility, auxin transport, and plant
development, including auxin transport-dependent processes. Our results show that
a class of ATIs act as actin stabilizers and advocate that actin-dependent trafficking
of auxin transport components participates in the mechanism of auxin transport.
These studies also provide an example of how the common eukaryotic process of
actin-based vesicle motility can fulfill a plant-specific physiological role.
author:
- first_name: Pankaj
full_name: Dhonukshe, Pankaj
last_name: Dhonukshe
- first_name: Ilya
full_name: Grigoriev, Ilya S
last_name: Grigoriev
- first_name: Rainer
full_name: Fischer, Rainer
last_name: Fischer
- first_name: Motoki
full_name: Tominaga, Motoki
last_name: Tominaga
- first_name: David
full_name: Robinson, David G
last_name: Robinson
- first_name: Jiří
full_name: Hašek, Jiří
last_name: Hašek
- first_name: Tomasz
full_name: Paciorek, Tomasz
last_name: Paciorek
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Daniela
full_name: Seifertová, Daniela
last_name: Seifertová
- first_name: Ricardo
full_name: Tejos, Ricardo
last_name: Tejos
- first_name: Lee
full_name: Meisel, Lee A
last_name: Meisel
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
- first_name: Theodorus
full_name: Gadella, Theodorus W
last_name: Gadella
- first_name: York
full_name: Stierhof, York-Dieter
last_name: Stierhof
- first_name: Takashi
full_name: Ueda, Takashi
last_name: Ueda
- first_name: Kazuhiro
full_name: Oiwa, Kazuhiro
last_name: Oiwa
- first_name: Anna
full_name: Akhmanova, Anna
last_name: Akhmanova
- first_name: Roland
full_name: Brock, Roland
last_name: Brock
- first_name: Anne
full_name: Spang, Anne
last_name: Spang
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Dhonukshe P, Grigoriev I, Fischer R, et al. Auxin transport inhibitors impair
vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes. PNAS.
2008;105(11):4489-4494. doi:10.1073/pnas.0711414105
apa: Dhonukshe, P., Grigoriev, I., Fischer, R., Tominaga, M., Robinson, D., Hašek,
J., … Friml, J. (2008). Auxin transport inhibitors impair vesicle motility and
actin cytoskeleton dynamics in diverse eukaryotes. PNAS. National Academy
of Sciences. https://doi.org/10.1073/pnas.0711414105
chicago: Dhonukshe, Pankaj, Ilya Grigoriev, Rainer Fischer, Motoki Tominaga, David
Robinson, Jiří Hašek, Tomasz Paciorek, et al. “Auxin Transport Inhibitors Impair
Vesicle Motility and Actin Cytoskeleton Dynamics in Diverse Eukaryotes.” PNAS.
National Academy of Sciences, 2008. https://doi.org/10.1073/pnas.0711414105.
ieee: P. Dhonukshe et al., “Auxin transport inhibitors impair vesicle motility
and actin cytoskeleton dynamics in diverse eukaryotes,” PNAS, vol. 105,
no. 11. National Academy of Sciences, pp. 4489–4494, 2008.
ista: Dhonukshe P, Grigoriev I, Fischer R, Tominaga M, Robinson D, Hašek J, Paciorek
T, Petrášek J, Seifertová D, Tejos R, Meisel L, Zažímalová E, Gadella T, Stierhof
Y, Ueda T, Oiwa K, Akhmanova A, Brock R, Spang A, Friml J. 2008. Auxin transport
inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse
eukaryotes. PNAS. 105(11), 4489–4494.
mla: Dhonukshe, Pankaj, et al. “Auxin Transport Inhibitors Impair Vesicle Motility
and Actin Cytoskeleton Dynamics in Diverse Eukaryotes.” PNAS, vol. 105,
no. 11, National Academy of Sciences, 2008, pp. 4489–94, doi:10.1073/pnas.0711414105.
short: P. Dhonukshe, I. Grigoriev, R. Fischer, M. Tominaga, D. Robinson, J. Hašek,
T. Paciorek, J. Petrášek, D. Seifertová, R. Tejos, L. Meisel, E. Zažímalová, T.
Gadella, Y. Stierhof, T. Ueda, K. Oiwa, A. Akhmanova, R. Brock, A. Spang, J. Friml,
PNAS 105 (2008) 4489–4494.
date_created: 2018-12-11T12:00:58Z
date_published: 2008-03-18T00:00:00Z
date_updated: 2021-01-12T07:40:34Z
day: '18'
doi: 10.1073/pnas.0711414105
extern: 1
intvolume: ' 105'
issue: '11'
month: '03'
page: 4489 - 4494
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '3672'
quality_controlled: 0
status: public
title: Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics
in diverse eukaryotes
type: journal_article
volume: 105
year: '2008'
...