--- _id: '3031' abstract: - lang: eng text: Many aspects of plant development, including patterning and tropisms, are largely dependent on the asymmetric distribution of the plant signaling molecule auxin. Auxin transport inhibitors (ATIs), which interfere with directional auxin transport, have been essential tools in formulating this concept. However, despite the use of ATIs in plant research for many decades, the mechanism of ATI action has remained largely elusive. Using real-time live-cell microscopy, we show here that prominent ATIs such as 2,3,5-triiodobenzoic acid (TIBA) and 2-(1-pyrenoyl) benzoic acid (PBA) inhibit vesicle trafficking in plant, yeast, and mammalian cells. Effects on micropinocytosis, rab5-labeled endosomal motility at the periphery of HeLa cells and on fibroblast mobility indicate that ATIs influence actin cytoskeleton. Visualization of actin cytoskeleton dynamics in plants, yeast, and mammalian cells show that ATIs stabilize actin. Conversely, stabilizing actin by chemical or genetic means interferes with endocytosis, vesicle motility, auxin transport, and plant development, including auxin transport-dependent processes. Our results show that a class of ATIs act as actin stabilizers and advocate that actin-dependent trafficking of auxin transport components participates in the mechanism of auxin transport. These studies also provide an example of how the common eukaryotic process of actin-based vesicle motility can fulfill a plant-specific physiological role. author: - first_name: Pankaj full_name: Dhonukshe, Pankaj last_name: Dhonukshe - first_name: Ilya full_name: Grigoriev, Ilya S last_name: Grigoriev - first_name: Rainer full_name: Fischer, Rainer last_name: Fischer - first_name: Motoki full_name: Tominaga, Motoki last_name: Tominaga - first_name: David full_name: Robinson, David G last_name: Robinson - first_name: Jiří full_name: Hašek, Jiří last_name: Hašek - first_name: Tomasz full_name: Paciorek, Tomasz last_name: Paciorek - first_name: Jan full_name: Petrášek, Jan last_name: Petrášek - first_name: Daniela full_name: Seifertová, Daniela last_name: Seifertová - first_name: Ricardo full_name: Tejos, Ricardo last_name: Tejos - first_name: Lee full_name: Meisel, Lee A last_name: Meisel - first_name: Eva full_name: Zažímalová, Eva last_name: Zažímalová - first_name: Theodorus full_name: Gadella, Theodorus W last_name: Gadella - first_name: York full_name: Stierhof, York-Dieter last_name: Stierhof - first_name: Takashi full_name: Ueda, Takashi last_name: Ueda - first_name: Kazuhiro full_name: Oiwa, Kazuhiro last_name: Oiwa - first_name: Anna full_name: Akhmanova, Anna last_name: Akhmanova - first_name: Roland full_name: Brock, Roland last_name: Brock - first_name: Anne full_name: Spang, Anne last_name: Spang - first_name: Jirí full_name: Jirí Friml id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Dhonukshe P, Grigoriev I, Fischer R, et al. Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes. PNAS. 2008;105(11):4489-4494. doi:10.1073/pnas.0711414105 apa: Dhonukshe, P., Grigoriev, I., Fischer, R., Tominaga, M., Robinson, D., Hašek, J., … Friml, J. (2008). Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.0711414105 chicago: Dhonukshe, Pankaj, Ilya Grigoriev, Rainer Fischer, Motoki Tominaga, David Robinson, Jiří Hašek, Tomasz Paciorek, et al. “Auxin Transport Inhibitors Impair Vesicle Motility and Actin Cytoskeleton Dynamics in Diverse Eukaryotes.” PNAS. National Academy of Sciences, 2008. https://doi.org/10.1073/pnas.0711414105. ieee: P. Dhonukshe et al., “Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes,” PNAS, vol. 105, no. 11. National Academy of Sciences, pp. 4489–4494, 2008. ista: Dhonukshe P, Grigoriev I, Fischer R, Tominaga M, Robinson D, Hašek J, Paciorek T, Petrášek J, Seifertová D, Tejos R, Meisel L, Zažímalová E, Gadella T, Stierhof Y, Ueda T, Oiwa K, Akhmanova A, Brock R, Spang A, Friml J. 2008. Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes. PNAS. 105(11), 4489–4494. mla: Dhonukshe, Pankaj, et al. “Auxin Transport Inhibitors Impair Vesicle Motility and Actin Cytoskeleton Dynamics in Diverse Eukaryotes.” PNAS, vol. 105, no. 11, National Academy of Sciences, 2008, pp. 4489–94, doi:10.1073/pnas.0711414105. short: P. Dhonukshe, I. Grigoriev, R. Fischer, M. Tominaga, D. Robinson, J. Hašek, T. Paciorek, J. Petrášek, D. Seifertová, R. Tejos, L. Meisel, E. Zažímalová, T. Gadella, Y. Stierhof, T. Ueda, K. Oiwa, A. Akhmanova, R. Brock, A. Spang, J. Friml, PNAS 105 (2008) 4489–4494. date_created: 2018-12-11T12:00:58Z date_published: 2008-03-18T00:00:00Z date_updated: 2021-01-12T07:40:34Z day: '18' doi: 10.1073/pnas.0711414105 extern: 1 intvolume: ' 105' issue: '11' month: '03' page: 4489 - 4494 publication: PNAS publication_status: published publisher: National Academy of Sciences publist_id: '3672' quality_controlled: 0 status: public title: Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes type: journal_article volume: 105 year: '2008' ...