Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes

P. Dhonukshe, I. Grigoriev, R. Fischer, M. Tominaga, D. Robinson, J. Hašek, T. Paciorek, J. Petrášek, D. Seifertová, R. Tejos, L. Meisel, E. Zažímalová, T. Gadella, Y. Stierhof, T. Ueda, K. Oiwa, A. Akhmanova, R. Brock, A. Spang, J. Friml, PNAS 105 (2008) 4489–4494.

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Abstract
Many aspects of plant development, including patterning and tropisms, are largely dependent on the asymmetric distribution of the plant signaling molecule auxin. Auxin transport inhibitors (ATIs), which interfere with directional auxin transport, have been essential tools in formulating this concept. However, despite the use of ATIs in plant research for many decades, the mechanism of ATI action has remained largely elusive. Using real-time live-cell microscopy, we show here that prominent ATIs such as 2,3,5-triiodobenzoic acid (TIBA) and 2-(1-pyrenoyl) benzoic acid (PBA) inhibit vesicle trafficking in plant, yeast, and mammalian cells. Effects on micropinocytosis, rab5-labeled endosomal motility at the periphery of HeLa cells and on fibroblast mobility indicate that ATIs influence actin cytoskeleton. Visualization of actin cytoskeleton dynamics in plants, yeast, and mammalian cells show that ATIs stabilize actin. Conversely, stabilizing actin by chemical or genetic means interferes with endocytosis, vesicle motility, auxin transport, and plant development, including auxin transport-dependent processes. Our results show that a class of ATIs act as actin stabilizers and advocate that actin-dependent trafficking of auxin transport components participates in the mechanism of auxin transport. These studies also provide an example of how the common eukaryotic process of actin-based vesicle motility can fulfill a plant-specific physiological role.
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Date Published
2008-03-18
Journal Title
PNAS
Volume
105
Issue
11
Page
4489 - 4494
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Dhonukshe P, Grigoriev I, Fischer R, et al. Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes. PNAS. 2008;105(11):4489-4494. doi:10.1073/pnas.0711414105
Dhonukshe, P., Grigoriev, I., Fischer, R., Tominaga, M., Robinson, D., Hašek, J., … Friml, J. (2008). Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes. PNAS, 105(11), 4489–4494. https://doi.org/10.1073/pnas.0711414105
Dhonukshe, Pankaj, Ilya Grigoriev, Rainer Fischer, Motoki Tominaga, David Robinson, Jiří Hašek, Tomasz Paciorek, et al. “Auxin Transport Inhibitors Impair Vesicle Motility and Actin Cytoskeleton Dynamics in Diverse Eukaryotes.” PNAS 105, no. 11 (2008): 4489–94. https://doi.org/10.1073/pnas.0711414105.
P. Dhonukshe et al., “Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes,” PNAS, vol. 105, no. 11, pp. 4489–4494, 2008.
Dhonukshe P, Grigoriev I, Fischer R, Tominaga M, Robinson D, Hašek J, Paciorek T, Petrášek J, Seifertová D, Tejos R, Meisel L, Zažímalová E, Gadella T, Stierhof Y, Ueda T, Oiwa K, Akhmanova A, Brock R, Spang A, Friml J. 2008. Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes. PNAS. 105(11), 4489–4494.
Dhonukshe, Pankaj, et al. “Auxin Transport Inhibitors Impair Vesicle Motility and Actin Cytoskeleton Dynamics in Diverse Eukaryotes.” PNAS, vol. 105, no. 11, National Academy of Sciences, 2008, pp. 4489–94, doi:10.1073/pnas.0711414105.

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