--- _id: '3028' abstract: - lang: eng text: In plants, cell polarity and tissue patterning are connected by intercellular flow of the phytohormone auxin, whose directional signaling depends on polar subcellular localization of PIN auxin transport proteins. The mechanism of polar targeting of PINs or other cargos in plants is largely unidentified, with the PINOID kinase being the only known molecular component. Here, we identify PP2A phosphatase as an important regulator of PIN apical-basal targeting and auxin distribution. Genetic analysis, localization, and phosphorylation studies demonstrate that PP2A and PINOID both partially colocalize with PINs and act antagonistically on the phosphorylation state of their central hydrophilic loop, hence mediating PIN apical-basal polar targeting. Thus, in plants, polar sorting by the reversible phosphorylation of cargos allows for their conditional delivery to specific intracellular destinations. In the case of PIN proteins, this mechanism enables switches in the direction of intercellular auxin fluxes, which mediate differential growth, tissue patterning, and organogenesis. author: - first_name: Marta full_name: Michniewicz, Marta last_name: Michniewicz - first_name: Marcelo full_name: Zago, Marcelo K last_name: Zago - first_name: Lindy full_name: Abas, Lindy last_name: Abas - first_name: Dolf full_name: Weijers, Dolf last_name: Weijers - first_name: Alois full_name: Schweighofer, Alois last_name: Schweighofer - first_name: Irute full_name: Meskiene, Irute last_name: Meskiene - first_name: Marcus full_name: Heisler, Marcus G last_name: Heisler - first_name: Carolyn full_name: Ohno, Carolyn last_name: Ohno - first_name: Jing full_name: Zhang, Jing last_name: Zhang - first_name: Fang full_name: Huang, Fang last_name: Huang - first_name: Rebecca full_name: Schwab, Rebecca last_name: Schwab - first_name: Detlef full_name: Weigel, Detlef last_name: Weigel - first_name: Elliot full_name: Meyerowitz, Elliot M last_name: Meyerowitz - first_name: Christian full_name: Luschnig, Christian last_name: Luschnig - first_name: Remko full_name: Offringa, Remko last_name: Offringa - first_name: Jirí full_name: Jirí Friml id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Michniewicz M, Zago M, Abas L, et al. Antagonistic regulation of PIN phosphorylation by PP2A and PINOID directs auxin flux. Cell. 2007;130(6):1044-1056. doi:10.1016/j.cell.2007.07.033 apa: Michniewicz, M., Zago, M., Abas, L., Weijers, D., Schweighofer, A., Meskiene, I., … Friml, J. (2007). Antagonistic regulation of PIN phosphorylation by PP2A and PINOID directs auxin flux. Cell. Cell Press. https://doi.org/10.1016/j.cell.2007.07.033 chicago: Michniewicz, Marta, Marcelo Zago, Lindy Abas, Dolf Weijers, Alois Schweighofer, Irute Meskiene, Marcus Heisler, et al. “Antagonistic Regulation of PIN Phosphorylation by PP2A and PINOID Directs Auxin Flux.” Cell. Cell Press, 2007. https://doi.org/10.1016/j.cell.2007.07.033. ieee: M. Michniewicz et al., “Antagonistic regulation of PIN phosphorylation by PP2A and PINOID directs auxin flux,” Cell, vol. 130, no. 6. Cell Press, pp. 1044–1056, 2007. ista: Michniewicz M, Zago M, Abas L, Weijers D, Schweighofer A, Meskiene I, Heisler M, Ohno C, Zhang J, Huang F, Schwab R, Weigel D, Meyerowitz E, Luschnig C, Offringa R, Friml J. 2007. Antagonistic regulation of PIN phosphorylation by PP2A and PINOID directs auxin flux. Cell. 130(6), 1044–1056. mla: Michniewicz, Marta, et al. “Antagonistic Regulation of PIN Phosphorylation by PP2A and PINOID Directs Auxin Flux.” Cell, vol. 130, no. 6, Cell Press, 2007, pp. 1044–56, doi:10.1016/j.cell.2007.07.033. short: M. Michniewicz, M. Zago, L. Abas, D. Weijers, A. Schweighofer, I. Meskiene, M. Heisler, C. Ohno, J. Zhang, F. Huang, R. Schwab, D. Weigel, E. Meyerowitz, C. Luschnig, R. Offringa, J. Friml, Cell 130 (2007) 1044–1056. date_created: 2018-12-11T12:00:57Z date_published: 2007-09-21T00:00:00Z date_updated: 2021-01-12T07:40:33Z day: '21' doi: 10.1016/j.cell.2007.07.033 extern: 1 intvolume: ' 130' issue: '6' month: '09' page: 1044 - 1056 publication: Cell publication_status: published publisher: Cell Press publist_id: '3674' quality_controlled: 0 status: public title: Antagonistic regulation of PIN phosphorylation by PP2A and PINOID directs auxin flux type: journal_article volume: 130 year: '2007' ...