Antagonistic regulation of PIN phosphorylation by PP2A and PINOID directs auxin flux

M. Michniewicz, M. Zago, L. Abas, D. Weijers, A. Schweighofer, I. Meskiene, M. Heisler, C. Ohno, J. Zhang, F. Huang, R. Schwab, D. Weigel, E. Meyerowitz, C. Luschnig, R. Offringa, J. Friml, Cell 130 (2007) 1044–1056.

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Abstract
In plants, cell polarity and tissue patterning are connected by intercellular flow of the phytohormone auxin, whose directional signaling depends on polar subcellular localization of PIN auxin transport proteins. The mechanism of polar targeting of PINs or other cargos in plants is largely unidentified, with the PINOID kinase being the only known molecular component. Here, we identify PP2A phosphatase as an important regulator of PIN apical-basal targeting and auxin distribution. Genetic analysis, localization, and phosphorylation studies demonstrate that PP2A and PINOID both partially colocalize with PINs and act antagonistically on the phosphorylation state of their central hydrophilic loop, hence mediating PIN apical-basal polar targeting. Thus, in plants, polar sorting by the reversible phosphorylation of cargos allows for their conditional delivery to specific intracellular destinations. In the case of PIN proteins, this mechanism enables switches in the direction of intercellular auxin fluxes, which mediate differential growth, tissue patterning, and organogenesis.
Publishing Year
Date Published
2007-09-21
Journal Title
Cell
Volume
130
Issue
6
Page
1044 - 1056
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Michniewicz M, Zago M, Abas L, et al. Antagonistic regulation of PIN phosphorylation by PP2A and PINOID directs auxin flux. Cell. 2007;130(6):1044-1056. doi:10.1016/j.cell.2007.07.033
Michniewicz, M., Zago, M., Abas, L., Weijers, D., Schweighofer, A., Meskiene, I., … Friml, J. (2007). Antagonistic regulation of PIN phosphorylation by PP2A and PINOID directs auxin flux. Cell, 130(6), 1044–1056. https://doi.org/10.1016/j.cell.2007.07.033
Michniewicz, Marta, Marcelo Zago, Lindy Abas, Dolf Weijers, Alois Schweighofer, Irute Meskiene, Marcus Heisler, et al. “Antagonistic Regulation of PIN Phosphorylation by PP2A and PINOID Directs Auxin Flux.” Cell 130, no. 6 (2007): 1044–56. https://doi.org/10.1016/j.cell.2007.07.033.
M. Michniewicz et al., “Antagonistic regulation of PIN phosphorylation by PP2A and PINOID directs auxin flux,” Cell, vol. 130, no. 6, pp. 1044–1056, 2007.
Michniewicz M, Zago M, Abas L, Weijers D, Schweighofer A, Meskiene I, Heisler M, Ohno C, Zhang J, Huang F, Schwab R, Weigel D, Meyerowitz E, Luschnig C, Offringa R, Friml J. 2007. Antagonistic regulation of PIN phosphorylation by PP2A and PINOID directs auxin flux. Cell. 130(6), 1044–1056.
Michniewicz, Marta, et al. “Antagonistic Regulation of PIN Phosphorylation by PP2A and PINOID Directs Auxin Flux.” Cell, vol. 130, no. 6, Cell Press, 2007, pp. 1044–56, doi:10.1016/j.cell.2007.07.033.

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