Sauer, Michael; Friml, JiríIST Austria
Mujib, Abdul; Šamaj, Jozef
Plant Cell Monographs
Arabidopsis thaliana is currently the most important model organism for basic molecular plant research. It is also a favourable model for developmental biology, as its embryogenesis follows a nearly invariant pattern of cell divisions and cell type specifications. Study of embryogenesis can involve genetic, physiological or biochemical approaches, but is always limited by the inaccessibility of the embryos which develop deep inside maternal tissue. Thus, for developmental studies, there is an increasing demand for methods which allow embryogenesis under artificial conditions, providing better accessibility to experimental manipulation. In this chapter, we address theoretical aspects of embryo culture, give some thoughts on which embryo culture system is suited best for which application and finally discuss three current methods which have been successfully used in Arabidopsis embryo culture. © 2006 Springer-Verlag Berlin Heidelberg.
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Sauer M, Friml J. In vitro culture of Arabidopsis embryos. In: Mujib A, Šamaj J, eds. Somatic Embryogenesis. Vol 2. Springer; 2006:343-354. doi:10.1007/7089_020
Sauer, M., & Friml, J. (2006). In vitro culture of Arabidopsis embryos. In A. Mujib & J. Šamaj (Eds.), Somatic Embryogenesis (Vol. 2, pp. 343–354). Springer. https://doi.org/10.1007/7089_020
Sauer, Michael, and Jiří Friml. “In Vitro Culture of Arabidopsis Embryos.” In Somatic Embryogenesis, edited by Abdul Mujib and Jozef Šamaj, 2:343–54. Springer, 2006. https://doi.org/10.1007/7089_020.
M. Sauer and J. Friml, “In vitro culture of Arabidopsis embryos,” in Somatic Embryogenesis, vol. 2, A. Mujib and J. Šamaj, Eds. Springer, 2006, pp. 343–354.
Sauer M, Friml J. 2006.In vitro culture of Arabidopsis embryos. In: Somatic Embryogenesis. Plant Cell Monographs, vol. 2, 343–354.
Sauer, Michael, and Jiří Friml. “In Vitro Culture of Arabidopsis Embryos.” Somatic Embryogenesis, edited by Abdul Mujib and Jozef Šamaj, vol. 2, Springer, 2006, pp. 343–54, doi:10.1007/7089_020.