Automated whole mount localisation techniques for plant seedlings

J. Friml, E. Benková, U. Mayer, K. Palme, G. Muster, Plant Journal 34 (2003) 115–124.

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Abstract
Plant biology is currently experiencing a growing demand for easy and reliable mRNA and protein localisation techniques. Here, we present novel whole mount in situ hybridisation and immunolocalisation protocols, suitable to localise mRNAs and proteins in Arabidopsis seedlings. We demonstrate that these methods can be used in different organs of Arabidopsis seedlings as well as in other plant species. In order to achieve better reproducibility and higher throughput, we modified these protocols for automation to be performed by a liquid handling robot. In addition, we show that other procedures such as reporter enzyme assays and tissue clearing can be similarly automated. We present examples of application of our protocols including mRNA localisation and proteins and epitope tag (co)localisations which demonstrate that these methods provide reliable and versatile tools for expression, localisation and anatomical studies in plants.
Publishing Year
Date Published
2003-04-01
Journal Title
Plant Journal
Volume
34
Issue
1
Page
115 - 124
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Friml J, Benková E, Mayer U, Palme K, Muster G. Automated whole mount localisation techniques for plant seedlings. Plant Journal. 2003;34(1):115-124. doi:10.1046/j.1365-313X.2003.01705.x
Friml, J., Benková, E., Mayer, U., Palme, K., & Muster, G. (2003). Automated whole mount localisation techniques for plant seedlings. Plant Journal, 34(1), 115–124. https://doi.org/10.1046/j.1365-313X.2003.01705.x
Friml, Jirí, Eva Benková, Ulrike Mayer, Klaus Palme, and Gerhard Muster. “Automated Whole Mount Localisation Techniques for Plant Seedlings.” Plant Journal 34, no. 1 (2003): 115–24. https://doi.org/10.1046/j.1365-313X.2003.01705.x.
J. Friml, E. Benková, U. Mayer, K. Palme, and G. Muster, “Automated whole mount localisation techniques for plant seedlings,” Plant Journal, vol. 34, no. 1, pp. 115–124, 2003.
Friml J, Benková E, Mayer U, Palme K, Muster G. 2003. Automated whole mount localisation techniques for plant seedlings. Plant Journal. 34(1), 115–124.
Friml, Jirí, et al. “Automated Whole Mount Localisation Techniques for Plant Seedlings.” Plant Journal, vol. 34, no. 1, Wiley-Blackwell, 2003, pp. 115–24, doi:10.1046/j.1365-313X.2003.01705.x.

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