Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth

A. Molendijk, F. Bischoff, C. Rajendrakumar, J. Friml, M. Braun, S. Gilroy, K. Palme, EMBO Journal 20 (2001) 2779–2788.


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Abstract
Plants contain a novel unique subfamily of Rho GTPases, vital components of cellular signalling networks. Here we report a general role for some members of this family in polarized plant growth processes. We show that Arabidopsis AtRop4 and AtRop6 encode functional GTPases with similar intrinsic GTP hydrolysis rates. We localized AtRop proteins in root meristem cells to the cross-wall and cell plate membranes. Polar localization of AtRops in trichoblasts specifies the growth sites for emerging root hairs. These sites were visible before budding and elongation of the Arabidopsis root hair when AtRops accumulated at their tips. Expression of constitutively active AtRop4 and AtRop6 mutant proteins in root hairs of transgenic Arabidopsis plants abolished polarized growth and delocalized the tip-focused Ca2+ gradient. Polar localization of AtRops was inhibited by brefeldin A, but not by other drugs such as latrunculin B, cytochalasin D or caffeine. Our results demonstrate a general function of AtRop GTPases in tip growth and in polar diffuse growth.
Publishing Year
Date Published
2001-06-01
Journal Title
EMBO Journal
Volume
20
Issue
11
Page
2779 - 2788
IST-REx-ID

Cite this

Molendijk A, Bischoff F, Rajendrakumar C, et al. Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth. EMBO Journal. 2001;20(11):2779-2788. doi:10.1093/emboj/20.11.2779
Molendijk, A., Bischoff, F., Rajendrakumar, C., Friml, J., Braun, M., Gilroy, S., & Palme, K. (2001). Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth. EMBO Journal, 20(11), 2779–2788. https://doi.org/10.1093/emboj/20.11.2779
Molendijk, Arthur, Friedrich Bischoff, Chadalavada Rajendrakumar, Jirí Friml, Markus Braun, Simon Gilroy, and Klaus Palme. “Arabidopsis Thaliana Rop GTPases Are Localized to Tips of Root Hairs and Control Polar Growth.” EMBO Journal 20, no. 11 (2001): 2779–88. https://doi.org/10.1093/emboj/20.11.2779.
A. Molendijk et al., “Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth,” EMBO Journal, vol. 20, no. 11, pp. 2779–2788, 2001.
Molendijk A, Bischoff F, Rajendrakumar C, Friml J, Braun M, Gilroy S, Palme K. 2001. Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth. EMBO Journal. 20(11), 2779–2788.
Molendijk, Arthur, et al. “Arabidopsis Thaliana Rop GTPases Are Localized to Tips of Root Hairs and Control Polar Growth.” EMBO Journal, vol. 20, no. 11, Wiley-Blackwell, 2001, pp. 2779–88, doi:10.1093/emboj/20.11.2779.

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