---
_id: '2943'
abstract:
- lang: eng
text: We examine whether the Escherichia coli chromosome is folded into a self-adherent
nucleoprotein complex, or alternately is a confined but otherwise unconstrained
self-avoiding polymer. We address this through in vivo visualization, using an
inducible GFP fusion to the nucleoid-associated protein Fis to non-specifically
decorate the entire chromosome. For a range of different growth conditions, the
chromosome is a compact structure that does not fill the volume of the cell, and
which moves from the new pole to the cell centre. During rapid growth, chromosome
segregation occurs well before cell division, with daughter chromosomes coupled
by a thin inter-daughter filament before complete segregation, whereas during
slow growth chromosomes stay adjacent until cell division occurs. Image correlation
analysis indicates that sub-nucleoid structure is stable on a 1min timescale,
comparable to the timescale for redistribution time measured for GFP-Fis after
photobleaching. Optical deconvolution and writhe calculation analysis indicate
that the nucleoid has a large-scale coiled organization rather than being an amorphous
mass. Our observations are consistent with the chromosome having a self-adherent
filament organization.
acknowledgement: We thank Professor Philippe Cluzel and Mr Lance Min for providing
advice and materials. Jeannette Chau provided technical support. Work at NU was
supported by NSF Grants DMR-0715099, MCB-1022117, DMR-1206868, DMR-0520513 and DMR-1121262
(NU-MRSEC), by NIH-NCI Grant U54CA143869-01 (NU-PS-OC) and by the Chicago Biomedical
Consortium with support from the Searle Funds at the Chicago Community Trust. Work
at UCLA was supported by NIH Grant GM038509.
author:
- first_name: Nastaran
full_name: Hadizadeh Yazdi, Nastaran
last_name: Hadizadeh Yazdi
- first_name: Calin C
full_name: Guet, Calin C
id: 47F8433E-F248-11E8-B48F-1D18A9856A87
last_name: Guet
orcid: 0000-0001-6220-2052
- first_name: Reid
full_name: Johnson, Reid
last_name: Johnson
- first_name: John
full_name: Marko, John
last_name: Marko
citation:
ama: Hadizadeh Yazdi N, Guet CC, Johnson R, Marko J. Variation of the folding and
dynamics of the Escherichia coli chromosome with growth conditions. Molecular
Microbiology. 2012;86(6):1318-1333. doi:10.1111/mmi.12071
apa: Hadizadeh Yazdi, N., Guet, C. C., Johnson, R., & Marko, J. (2012). Variation
of the folding and dynamics of the Escherichia coli chromosome with growth conditions.
Molecular Microbiology. Wiley-Blackwell. https://doi.org/10.1111/mmi.12071
chicago: Hadizadeh Yazdi, Nastaran, Calin C Guet, Reid Johnson, and John Marko.
“Variation of the Folding and Dynamics of the Escherichia Coli Chromosome with
Growth Conditions.” Molecular Microbiology. Wiley-Blackwell, 2012. https://doi.org/10.1111/mmi.12071.
ieee: N. Hadizadeh Yazdi, C. C. Guet, R. Johnson, and J. Marko, “Variation of the
folding and dynamics of the Escherichia coli chromosome with growth conditions,”
Molecular Microbiology, vol. 86, no. 6. Wiley-Blackwell, pp. 1318–1333,
2012.
ista: Hadizadeh Yazdi N, Guet CC, Johnson R, Marko J. 2012. Variation of the folding
and dynamics of the Escherichia coli chromosome with growth conditions. Molecular
Microbiology. 86(6), 1318–1333.
mla: Hadizadeh Yazdi, Nastaran, et al. “Variation of the Folding and Dynamics of
the Escherichia Coli Chromosome with Growth Conditions.” Molecular Microbiology,
vol. 86, no. 6, Wiley-Blackwell, 2012, pp. 1318–33, doi:10.1111/mmi.12071.
short: N. Hadizadeh Yazdi, C.C. Guet, R. Johnson, J. Marko, Molecular Microbiology
86 (2012) 1318–1333.
date_created: 2018-12-11T12:00:28Z
date_published: 2012-11-09T00:00:00Z
date_updated: 2021-01-12T07:39:56Z
day: '09'
department:
- _id: CaGu
doi: 10.1111/mmi.12071
intvolume: ' 86'
issue: '6'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://europepmc.org/articles/pmc3524407
month: '11'
oa: 1
oa_version: Submitted Version
page: 1318 - 1333
publication: Molecular Microbiology
publication_status: published
publisher: Wiley-Blackwell
publist_id: '3790'
quality_controlled: '1'
scopus_import: 1
status: public
title: Variation of the folding and dynamics of the Escherichia coli chromosome with
growth conditions
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 86
year: '2012'
...