{"oa_version":"Submitted Version","year":"2012","acknowledgement":"We thank Professor Philippe Cluzel and Mr Lance Min for providing advice and materials. Jeannette Chau provided technical support. Work at NU was supported by NSF Grants DMR-0715099, MCB-1022117, DMR-1206868, DMR-0520513 and DMR-1121262 (NU-MRSEC), by NIH-NCI Grant U54CA143869-01 (NU-PS-OC) and by the Chicago Biomedical Consortium with support from the Searle Funds at the Chicago Community Trust. Work at UCLA was supported by NIH Grant GM038509.","main_file_link":[{"open_access":"1","url":"http://europepmc.org/articles/pmc3524407"}],"month":"11","quality_controlled":"1","abstract":[{"text":"We examine whether the Escherichia coli chromosome is folded into a self-adherent nucleoprotein complex, or alternately is a confined but otherwise unconstrained self-avoiding polymer. We address this through in vivo visualization, using an inducible GFP fusion to the nucleoid-associated protein Fis to non-specifically decorate the entire chromosome. For a range of different growth conditions, the chromosome is a compact structure that does not fill the volume of the cell, and which moves from the new pole to the cell centre. During rapid growth, chromosome segregation occurs well before cell division, with daughter chromosomes coupled by a thin inter-daughter filament before complete segregation, whereas during slow growth chromosomes stay adjacent until cell division occurs. Image correlation analysis indicates that sub-nucleoid structure is stable on a 1min timescale, comparable to the timescale for redistribution time measured for GFP-Fis after photobleaching. Optical deconvolution and writhe calculation analysis indicate that the nucleoid has a large-scale coiled organization rather than being an amorphous mass. Our observations are consistent with the chromosome having a self-adherent filament organization.","lang":"eng"}],"date_updated":"2021-01-12T07:39:56Z","publication_status":"published","intvolume":"        86","title":"Variation of the folding and dynamics of the Escherichia coli chromosome with growth conditions","publisher":"Wiley-Blackwell","language":[{"iso":"eng"}],"date_published":"2012-11-09T00:00:00Z","issue":"6","date_created":"2018-12-11T12:00:28Z","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","citation":{"ieee":"N. Hadizadeh Yazdi, C. C. Guet, R. Johnson, and J. Marko, “Variation of the folding and dynamics of the Escherichia coli chromosome with growth conditions,” <i>Molecular Microbiology</i>, vol. 86, no. 6. Wiley-Blackwell, pp. 1318–1333, 2012.","mla":"Hadizadeh Yazdi, Nastaran, et al. “Variation of the Folding and Dynamics of the Escherichia Coli Chromosome with Growth Conditions.” <i>Molecular Microbiology</i>, vol. 86, no. 6, Wiley-Blackwell, 2012, pp. 1318–33, doi:<a href=\"https://doi.org/10.1111/mmi.12071\">10.1111/mmi.12071</a>.","ista":"Hadizadeh Yazdi N, Guet CC, Johnson R, Marko J. 2012. Variation of the folding and dynamics of the Escherichia coli chromosome with growth conditions. Molecular Microbiology. 86(6), 1318–1333.","ama":"Hadizadeh Yazdi N, Guet CC, Johnson R, Marko J. Variation of the folding and dynamics of the Escherichia coli chromosome with growth conditions. <i>Molecular Microbiology</i>. 2012;86(6):1318-1333. doi:<a href=\"https://doi.org/10.1111/mmi.12071\">10.1111/mmi.12071</a>","apa":"Hadizadeh Yazdi, N., Guet, C. C., Johnson, R., &#38; Marko, J. (2012). Variation of the folding and dynamics of the Escherichia coli chromosome with growth conditions. <i>Molecular Microbiology</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1111/mmi.12071\">https://doi.org/10.1111/mmi.12071</a>","chicago":"Hadizadeh Yazdi, Nastaran, Calin C Guet, Reid Johnson, and John Marko. “Variation of the Folding and Dynamics of the Escherichia Coli Chromosome with Growth Conditions.” <i>Molecular Microbiology</i>. Wiley-Blackwell, 2012. <a href=\"https://doi.org/10.1111/mmi.12071\">https://doi.org/10.1111/mmi.12071</a>.","short":"N. Hadizadeh Yazdi, C.C. Guet, R. Johnson, J. Marko, Molecular Microbiology 86 (2012) 1318–1333."},"page":"1318 - 1333","publication":"Molecular Microbiology","volume":86,"scopus_import":1,"department":[{"_id":"CaGu"}],"type":"journal_article","publist_id":"3790","status":"public","_id":"2943","author":[{"first_name":"Nastaran","full_name":"Hadizadeh Yazdi, Nastaran","last_name":"Hadizadeh Yazdi"},{"full_name":"Guet, Calin C","first_name":"Calin C","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","last_name":"Guet","orcid":"0000-0001-6220-2052"},{"first_name":"Reid","full_name":"Johnson, Reid","last_name":"Johnson"},{"last_name":"Marko","first_name":"John","full_name":"Marko, John"}],"day":"09","doi":"10.1111/mmi.12071","oa":1}