Quantitative regional and ultra structural localization of the Ca v2 3 subunit of R type calcium channel in mouse brain

L. Parajuli, C. Nakajima, Á. Kulik, K. Matsui, T. Schneider, R. Shigemoto, Y. Fukazawa, Journal of Neuroscience 32 (2012) 13555–13567.

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Author
Parajuli, Laxmi K; Nakajima, Chikako; Kulik, Ákos; Matsui, Ko; Schneider, Toni; Shigemoto, RyuichiIST Austria ; Fukazawa, Yugo
Abstract
R-type calcium channels (RTCCs) are well known for their role in synaptic plasticity, but little is known about their subcellular distribution across various neuronal compartments. Using subtype-specific antibodies, we characterized the regional and subcellular localization of Ca v2.3 in mice and rats at both light and electron microscopic levels. Ca v2.3 immunogold particles were found to be predominantly presynaptic in the interpeduncular nucleus, but postsynaptic in other brain regions. Serial section analysis of electron microscopic images from the hippocampal CA1 revealed a higher density of immunogold particles in the dendritic shaft plasma membrane compared with the pyramidal cell somata. However, the labeling densities were not significantly different among the apical, oblique, or basal dendrites. Immunogold particles were also observed over the plasma membrane of dendritic spines, including both synaptic and extrasynaptic sites. Individual spine heads contained <20 immunogold particles, with an average density of ~260 immunoparticles per μm 3 spine head volume, in accordance with the density of RTCCs estimated using calcium imaging (Sabatini and Svoboda, 2000). The Ca v2.3 density was variable among similar-sized spine heads and did not correlate with the density in the parent dendrite, implying that spines are individual calcium compartments operating autonomously from their parent dendrites.
Publishing Year
Date Published
2012-09-26
Journal Title
Journal of Neuroscience
Volume
32
Issue
39
Page
13555 - 13567
IST-REx-ID

Cite this

Parajuli L, Nakajima C, Kulik Á, et al. Quantitative regional and ultra structural localization of the Ca v2 3 subunit of R type calcium channel in mouse brain. Journal of Neuroscience. 2012;32(39):13555-13567. doi:10.1523/JNEUROSCI.1142-12.2012
Parajuli, L., Nakajima, C., Kulik, Á., Matsui, K., Schneider, T., Shigemoto, R., & Fukazawa, Y. (2012). Quantitative regional and ultra structural localization of the Ca v2 3 subunit of R type calcium channel in mouse brain. Journal of Neuroscience, 32(39), 13555–13567. https://doi.org/10.1523/JNEUROSCI.1142-12.2012
Parajuli, Laxmi, Chikako Nakajima, Ákos Kulik, Ko Matsui, Toni Schneider, Ryuichi Shigemoto, and Yugo Fukazawa. “Quantitative Regional and Ultra Structural Localization of the Ca v2 3 Subunit of R Type Calcium Channel in Mouse Brain.” Journal of Neuroscience 32, no. 39 (2012): 13555–67. https://doi.org/10.1523/JNEUROSCI.1142-12.2012.
L. Parajuli et al., “Quantitative regional and ultra structural localization of the Ca v2 3 subunit of R type calcium channel in mouse brain,” Journal of Neuroscience, vol. 32, no. 39, pp. 13555–13567, 2012.
Parajuli L, Nakajima C, Kulik Á, Matsui K, Schneider T, Shigemoto R, Fukazawa Y. 2012. Quantitative regional and ultra structural localization of the Ca v2 3 subunit of R type calcium channel in mouse brain. Journal of Neuroscience. 32(39), 13555–13567.
Parajuli, Laxmi, et al. “Quantitative Regional and Ultra Structural Localization of the Ca v2 3 Subunit of R Type Calcium Channel in Mouse Brain.” Journal of Neuroscience, vol. 32, no. 39, Society for Neuroscience, 2012, pp. 13555–67, doi:10.1523/JNEUROSCI.1142-12.2012.

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