--- res: bibo_abstract: - G-protein-coupled inwardly rectifying K+ channels (Kir3 channels) coupled to metabotropic GABAB receptors are essential for the control of neuronal excitation. To determine the distribution of Kir3 channels and their spatial relationship to GABAB receptors on hippocampal pyramidal cells, we used a high-resolution immunocytochemical approach. Immunoreactivity for the Kir3.2 subunit was most abundant postsynaptically and localized to the extrasynaptic plasma membrane of dendritic shafts and spines of principal cells. Quantitative analysis of immunogold particles for Kir3.2 revealed an enrichment of the protein around putative glutamatergic synapses on dendritic spines, similar to that of GABA B1. Consistent with this observation, a high degree of coclustering of Kir3.2 and GABAB1 was revealed around excitatory synapses by the highly sensitive SDS-digested freeze-fracture replica immunolabeling. In contrast, in dendritic shafts receptors and channels were found to be mainly segregated. These results suggest that Kir3.2-containing K+ channels on dendritic spines preferentially mediate the effect of GABA, whereas channels on dendritic shafts are likely to be activated by other neurotransmitters as well. Thus, Kir3 channels, localized to different subcellular compartments of hippocampal principal cells, appear to be differentially involved in synaptic integration in pyramidal cell dendrites.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Ákos foaf_name: Kulik, Ákos foaf_surname: Kulik - foaf_Person: foaf_givenName: Imre foaf_name: Vida, Imre foaf_surname: Vida - foaf_Person: foaf_givenName: Yugo foaf_name: Fukazawa, Yugo foaf_surname: Fukazawa - foaf_Person: foaf_givenName: Nicole foaf_name: Guetg, Nicole foaf_surname: Guetg - foaf_Person: foaf_givenName: Yu foaf_name: Kasugai, Yu foaf_surname: Kasugai - foaf_Person: foaf_givenName: Cheryl foaf_name: Marker, Cheryl L foaf_surname: Marker - foaf_Person: foaf_givenName: Franck foaf_name: Rigato, Franck foaf_surname: Rigato - foaf_Person: foaf_givenName: Bernhard foaf_name: Bettler, Bernhard foaf_surname: Bettler - foaf_Person: foaf_givenName: Kevin foaf_name: Wickman, Kevin D foaf_surname: Wickman - foaf_Person: foaf_givenName: Michael foaf_name: Frotscher, Michael foaf_surname: Frotscher - foaf_Person: foaf_givenName: Ryuichi foaf_name: Ryuichi Shigemoto foaf_surname: Shigemoto foaf_workInfoHomepage: http://www.librecat.org/personId=499F3ABC-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-8761-9444 bibo_doi: 10.1523/JNEUROSCI.4178-05.2006 bibo_issue: '16' bibo_volume: 26 dct_date: 2006^xs_gYear dct_publisher: Society for Neuroscience@ dct_title: Compartment-dependent colocalization of Kir3.2-containing K+ channels and GABAB receptors in hippocampal pyramidal cells@ ...