Compartment-dependent colocalization of Kir3.2-containing K+ channels and GABAB receptors in hippocampal pyramidal cells

Á. Kulik, I. Vida, Y. Fukazawa, N. Guetg, Y. Kasugai, C. Marker, F. Rigato, B. Bettler, K. Wickman, M. Frotscher, R. Shigemoto, Journal of Neuroscience 26 (2006) 4289–4297.

Download
No fulltext has been uploaded. References only!

Journal Article | Published
Author
; ; ; ; ; ; ; ; ; ;
Abstract
G-protein-coupled inwardly rectifying K+ channels (Kir3 channels) coupled to metabotropic GABAB receptors are essential for the control of neuronal excitation. To determine the distribution of Kir3 channels and their spatial relationship to GABAB receptors on hippocampal pyramidal cells, we used a high-resolution immunocytochemical approach. Immunoreactivity for the Kir3.2 subunit was most abundant postsynaptically and localized to the extrasynaptic plasma membrane of dendritic shafts and spines of principal cells. Quantitative analysis of immunogold particles for Kir3.2 revealed an enrichment of the protein around putative glutamatergic synapses on dendritic spines, similar to that of GABA B1. Consistent with this observation, a high degree of coclustering of Kir3.2 and GABAB1 was revealed around excitatory synapses by the highly sensitive SDS-digested freeze-fracture replica immunolabeling. In contrast, in dendritic shafts receptors and channels were found to be mainly segregated. These results suggest that Kir3.2-containing K+ channels on dendritic spines preferentially mediate the effect of GABA, whereas channels on dendritic shafts are likely to be activated by other neurotransmitters as well. Thus, Kir3 channels, localized to different subcellular compartments of hippocampal principal cells, appear to be differentially involved in synaptic integration in pyramidal cell dendrites.
Publishing Year
Date Published
2006-04-19
Journal Title
Journal of Neuroscience
Volume
26
Issue
16
Page
4289 - 4297
IST-REx-ID

Cite this

Kulik Á, Vida I, Fukazawa Y, et al. Compartment-dependent colocalization of Kir3.2-containing K+ channels and GABAB receptors in hippocampal pyramidal cells. Journal of Neuroscience. 2006;26(16):4289-4297. doi:10.1523/JNEUROSCI.4178-05.2006
Kulik, Á., Vida, I., Fukazawa, Y., Guetg, N., Kasugai, Y., Marker, C., … Shigemoto, R. (2006). Compartment-dependent colocalization of Kir3.2-containing K+ channels and GABAB receptors in hippocampal pyramidal cells. Journal of Neuroscience, 26(16), 4289–4297. https://doi.org/10.1523/JNEUROSCI.4178-05.2006
Kulik, Ákos, Imre Vida, Yugo Fukazawa, Nicole Guetg, Yu Kasugai, Cheryl Marker, Franck Rigato, et al. “Compartment-Dependent Colocalization of Kir3.2-Containing K+ Channels and GABAB Receptors in Hippocampal Pyramidal Cells.” Journal of Neuroscience 26, no. 16 (2006): 4289–97. https://doi.org/10.1523/JNEUROSCI.4178-05.2006.
Á. Kulik et al., “Compartment-dependent colocalization of Kir3.2-containing K+ channels and GABAB receptors in hippocampal pyramidal cells,” Journal of Neuroscience, vol. 26, no. 16, pp. 4289–4297, 2006.
Kulik Á, Vida I, Fukazawa Y, Guetg N, Kasugai Y, Marker C, Rigato F, Bettler B, Wickman K, Frotscher M, Shigemoto R. 2006. Compartment-dependent colocalization of Kir3.2-containing K+ channels and GABAB receptors in hippocampal pyramidal cells. Journal of Neuroscience. 26(16), 4289–4297.
Kulik, Ákos, et al. “Compartment-Dependent Colocalization of Kir3.2-Containing K+ Channels and GABAB Receptors in Hippocampal Pyramidal Cells.” Journal of Neuroscience, vol. 26, no. 16, Society for Neuroscience, 2006, pp. 4289–97, doi:10.1523/JNEUROSCI.4178-05.2006.

Export

Marked Publications

Open Data IST Research Explorer

Search this title in

Google Scholar