Differential localization and regulation of stargazin-like protein, γ-8 and stargazin in the plasma membrane of hippocampal and cortical neurons

M. Inamura, M. Itakura, H. Okamoto, S. Hoka, A. Mizoguchi, Y. Fukazawa, R. Shigemoto, S. Yamamori, M. Takahashi, Neuroscience Research 55 (2006) 45–53.

Download
No fulltext has been uploaded. References only!

Journal Article | Published
Author
; ; ; ; ; ; ; ;
Abstract
Transmembrane AMPA receptor regulatory proteins (TARPs), including stargazin/γ-2, are associated with AMPA receptors and participate in their surface delivery and anchoring at the postsynaptic membrane. TARPs may also act as a positive modulator of the AMPA receptor ion channel function; however, little is known about other TARP members except for stargazin/γ-2. We examined the synaptic localization of stargazin/γ-2 and γ-8 by immunoelectron microscopy and biochemical analysis. The analysis of sodium dodecyl sulfate-digested freeze-fracture replica labeling revealed that stargazin/γ-2 was concentrated in the postsynaptic area, whereas γ-8 was distributed both in synaptic and extra-synaptic plasma membranes of the hippocampal neuron. When a synaptic plasma membrane-enriched brain fraction was treated with Triton X-100 and separated by sucrose density gradient ultracentrifugation, a large proportion of NMDA receptor and stargazin/γ-2 was accumulated in raft-enriched fractions, whereas AMPA receptor and γ-8 were distributed in both the raft-enriched fractions and other Triton-insoluble fractions. Phosphorylation of stargazin/γ-2 and γ-8 was regulated by different sets of kinases and phosphatases in cultured cortical neurons. These results suggested that stargazin/γ-2 and γ-8 have distinct roles in postsynaptic membranes under the regulation of different intracellular signaling pathways.
Publishing Year
Date Published
2006-05-01
Journal Title
Neuroscience Research
Volume
55
Issue
1
Page
45 - 53
IST-REx-ID

Cite this

Inamura M, Itakura M, Okamoto H, et al. Differential localization and regulation of stargazin-like protein, γ-8 and stargazin in the plasma membrane of hippocampal and cortical neurons. Neuroscience Research. 2006;55(1):45-53. doi:10.1016/j.neures.2006.01.004
Inamura, M., Itakura, M., Okamoto, H., Hoka, S., Mizoguchi, A., Fukazawa, Y., … Takahashi, M. (2006). Differential localization and regulation of stargazin-like protein, γ-8 and stargazin in the plasma membrane of hippocampal and cortical neurons. Neuroscience Research, 55(1), 45–53. https://doi.org/10.1016/j.neures.2006.01.004
Inamura, Mihoko, Makoto Itakura, Hirotsugu Okamoto, Sumio Hoka, Akira Mizoguchi, Yugo Fukazawa, Ryuichi Shigemoto, Saori Yamamori, and Masami Takahashi. “ Differential Localization and Regulation of Stargazin-like Protein, γ-8 and Stargazin in the Plasma Membrane of Hippocampal and Cortical Neurons.” Neuroscience Research 55, no. 1 (2006): 45–53. https://doi.org/10.1016/j.neures.2006.01.004.
M. Inamura et al., “ Differential localization and regulation of stargazin-like protein, γ-8 and stargazin in the plasma membrane of hippocampal and cortical neurons,” Neuroscience Research, vol. 55, no. 1, pp. 45–53, 2006.
Inamura M, Itakura M, Okamoto H, Hoka S, Mizoguchi A, Fukazawa Y, Shigemoto R, Yamamori S, Takahashi M. 2006. Differential localization and regulation of stargazin-like protein, γ-8 and stargazin in the plasma membrane of hippocampal and cortical neurons. Neuroscience Research. 55(1), 45–53.
Inamura, Mihoko, et al. “ Differential Localization and Regulation of Stargazin-like Protein, γ-8 and Stargazin in the Plasma Membrane of Hippocampal and Cortical Neurons.” Neuroscience Research, vol. 55, no. 1, Elsevier, 2006, pp. 45–53, doi:10.1016/j.neures.2006.01.004.

Export

Marked Publications

Open Data IST Research Explorer

Search this title in

Google Scholar