--- _id: '2657' abstract: - lang: eng text: The highest densities of the two metabotropic GABA subunits, GABA B1 and GABAB2, have been reported as occurring around the glutamatergic synapses between Purkinje cell spines and parallel fibre varicosities. In order to determine how this distribution is achieved during development, we investigated the expression pattern and the cellular and subcellular localization of the GABAB1 and GABAB2 subunits in the rat cerebellum during postnatal development. At the light microscopic level, immunoreactivity for the GABAB1 and GABAB2 subunits was very prominent in the developing molecular layer, especially in Purkinje cells. Using double immunofluorescence, we demonstrated that GABAB1 was transiently expressed in glial cells. At the electron microscopic level, immunoreactivity for GABAB receptors was always detected both pre- and postsynaptically. Presynaptically, GABAB1 and GABAB2 were localized in the extrasynaptic membrane of parallel fibres at all ages, and only rarely in GABAergic axons. Postsynaptically, GABAB receptors were localized to the extrasynaptic and perisynaptic plasma membrane of Purkinje cell dendrites and spines throughout development. Quantitative analysis and three-dimensional reconstructions further revealed a progressive developmental movement of the GABAB1 subunit on the surface of Purkinje cells from dendritic shafts to its final destination, the dendritic spines. Together, these results indicate that GABAB receptors undergo dynamic regulation during cerebellar development in association with the establishment and maturation of glutamatergic synapses to Purkinje cells. author: - first_name: Rafael full_name: Luján, Rafael last_name: Luján - first_name: Ryuichi full_name: Ryuichi Shigemoto id: 499F3ABC-F248-11E8-B48F-1D18A9856A87 last_name: Shigemoto orcid: 0000-0001-8761-9444 citation: ama: Luján R, Shigemoto R. Localization of metabotropic GABA receptor subunits GABAB1 and GABAB2 relative to synaptic sites in the rat developing cerebellum. European Journal of Neuroscience. 2006;23(6):1479-1490. doi:10.1111/j.1460-9568.2006.04669.x apa: Luján, R., & Shigemoto, R. (2006). Localization of metabotropic GABA receptor subunits GABAB1 and GABAB2 relative to synaptic sites in the rat developing cerebellum. European Journal of Neuroscience. Wiley-Blackwell. https://doi.org/10.1111/j.1460-9568.2006.04669.x chicago: Luján, Rafael, and Ryuichi Shigemoto. “Localization of Metabotropic GABA Receptor Subunits GABAB1 and GABAB2 Relative to Synaptic Sites in the Rat Developing Cerebellum.” European Journal of Neuroscience. Wiley-Blackwell, 2006. https://doi.org/10.1111/j.1460-9568.2006.04669.x. ieee: R. Luján and R. Shigemoto, “Localization of metabotropic GABA receptor subunits GABAB1 and GABAB2 relative to synaptic sites in the rat developing cerebellum,” European Journal of Neuroscience, vol. 23, no. 6. Wiley-Blackwell, pp. 1479–1490, 2006. ista: Luján R, Shigemoto R. 2006. Localization of metabotropic GABA receptor subunits GABAB1 and GABAB2 relative to synaptic sites in the rat developing cerebellum. European Journal of Neuroscience. 23(6), 1479–1490. mla: Luján, Rafael, and Ryuichi Shigemoto. “Localization of Metabotropic GABA Receptor Subunits GABAB1 and GABAB2 Relative to Synaptic Sites in the Rat Developing Cerebellum.” European Journal of Neuroscience, vol. 23, no. 6, Wiley-Blackwell, 2006, pp. 1479–90, doi:10.1111/j.1460-9568.2006.04669.x. short: R. Luján, R. Shigemoto, European Journal of Neuroscience 23 (2006) 1479–1490. date_created: 2018-12-11T11:58:54Z date_published: 2006-03-01T00:00:00Z date_updated: 2021-01-12T06:58:52Z day: '01' doi: 10.1111/j.1460-9568.2006.04669.x extern: 1 intvolume: ' 23' issue: '6' month: '03' page: 1479 - 1490 publication: European Journal of Neuroscience publication_status: published publisher: Wiley-Blackwell publist_id: '4239' quality_controlled: 0 status: public title: Localization of metabotropic GABA receptor subunits GABAB1 and GABAB2 relative to synaptic sites in the rat developing cerebellum type: journal_article volume: 23 year: '2006' ...