---
_id: '2657'
abstract:
- lang: eng
text: The highest densities of the two metabotropic GABA subunits, GABA B1 and GABAB2,
have been reported as occurring around the glutamatergic synapses between Purkinje
cell spines and parallel fibre varicosities. In order to determine how this distribution
is achieved during development, we investigated the expression pattern and the
cellular and subcellular localization of the GABAB1 and GABAB2 subunits in the
rat cerebellum during postnatal development. At the light microscopic level, immunoreactivity
for the GABAB1 and GABAB2 subunits was very prominent in the developing molecular
layer, especially in Purkinje cells. Using double immunofluorescence, we demonstrated
that GABAB1 was transiently expressed in glial cells. At the electron microscopic
level, immunoreactivity for GABAB receptors was always detected both pre- and
postsynaptically. Presynaptically, GABAB1 and GABAB2 were localized in the extrasynaptic
membrane of parallel fibres at all ages, and only rarely in GABAergic axons. Postsynaptically,
GABAB receptors were localized to the extrasynaptic and perisynaptic plasma membrane
of Purkinje cell dendrites and spines throughout development. Quantitative analysis
and three-dimensional reconstructions further revealed a progressive developmental
movement of the GABAB1 subunit on the surface of Purkinje cells from dendritic
shafts to its final destination, the dendritic spines. Together, these results
indicate that GABAB receptors undergo dynamic regulation during cerebellar development
in association with the establishment and maturation of glutamatergic synapses
to Purkinje cells.
author:
- first_name: Rafael
full_name: Luján, Rafael
last_name: Luján
- first_name: Ryuichi
full_name: Ryuichi Shigemoto
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
citation:
ama: Luján R, Shigemoto R. Localization of metabotropic GABA receptor subunits GABAB1
and GABAB2 relative to synaptic sites in the rat developing cerebellum. European
Journal of Neuroscience. 2006;23(6):1479-1490. doi:10.1111/j.1460-9568.2006.04669.x
apa: Luján, R., & Shigemoto, R. (2006). Localization of metabotropic GABA receptor
subunits GABAB1 and GABAB2 relative to synaptic sites in the rat developing cerebellum.
European Journal of Neuroscience. Wiley-Blackwell. https://doi.org/10.1111/j.1460-9568.2006.04669.x
chicago: Luján, Rafael, and Ryuichi Shigemoto. “Localization of Metabotropic GABA
Receptor Subunits GABAB1 and GABAB2 Relative to Synaptic Sites in the Rat Developing
Cerebellum.” European Journal of Neuroscience. Wiley-Blackwell, 2006. https://doi.org/10.1111/j.1460-9568.2006.04669.x.
ieee: R. Luján and R. Shigemoto, “Localization of metabotropic GABA receptor subunits
GABAB1 and GABAB2 relative to synaptic sites in the rat developing cerebellum,”
European Journal of Neuroscience, vol. 23, no. 6. Wiley-Blackwell, pp.
1479–1490, 2006.
ista: Luján R, Shigemoto R. 2006. Localization of metabotropic GABA receptor subunits
GABAB1 and GABAB2 relative to synaptic sites in the rat developing cerebellum.
European Journal of Neuroscience. 23(6), 1479–1490.
mla: Luján, Rafael, and Ryuichi Shigemoto. “Localization of Metabotropic GABA Receptor
Subunits GABAB1 and GABAB2 Relative to Synaptic Sites in the Rat Developing Cerebellum.”
European Journal of Neuroscience, vol. 23, no. 6, Wiley-Blackwell, 2006,
pp. 1479–90, doi:10.1111/j.1460-9568.2006.04669.x.
short: R. Luján, R. Shigemoto, European Journal of Neuroscience 23 (2006) 1479–1490.
date_created: 2018-12-11T11:58:54Z
date_published: 2006-03-01T00:00:00Z
date_updated: 2021-01-12T06:58:52Z
day: '01'
doi: 10.1111/j.1460-9568.2006.04669.x
extern: 1
intvolume: ' 23'
issue: '6'
month: '03'
page: 1479 - 1490
publication: European Journal of Neuroscience
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4239'
quality_controlled: 0
status: public
title: Localization of metabotropic GABA receptor subunits GABAB1 and GABAB2 relative
to synaptic sites in the rat developing cerebellum
type: journal_article
volume: 23
year: '2006'
...