Cellular localization of GABA and GABAB receptor subunit proteins during spermiogenesis in rat testis

K. Kanbara, K. Okamoto, S. Nomura, T. Kaneko, R. Shigemoto, H. Azuma, Y. Katsuoka, M. Watanabe, Journal of Andrology 26 (2005) 485–493.

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Kanbara, Kiyoto; Okamoto, Keiko; Nomura, Sakashi; Kaneko, Takeshi; Shigemoto, RyuichiIST Austria ; Azuma, Haruhito; Katsuoka, Yoji; Watanabe, Masahiko
Abstract
The GABAergic system, a major inhibitory regulator in the central nervous system, may also play important roles in peripheral nonneuronal tissues and cells. Recent studies showed that GABAB receptor is expressed in testis and sperm. To understand the role of the GABAergic system in spermiogenesis, we examined cellular localization of GABA and GABAB receptor subunits in rat spermatids by immunocytochemistry. Immunoreactivity for GABA was detected around acrosomal granules of spermatids during the Golgi and cap phases. GABAB(1) immunoreactivity was observed in the acrosomal vesicle of spermatids in Golgi phase, and during cap phase, this reactivity expanded to the entire region of the acrosome covering the nuclear membrane. The level of reactivity decreased gradually with maturation of spermatids. In contrast, GABAB(2) immunoreactivity was not observed in spermatids during Golgi phase but was detected in the equatorial region during cap phase. Both GABA immunoreactivity and GABAB(2) immunoreactivity were transferred to the residual cytoplasm during the release of spermatozoa. Electron microscopic immunocytochemistry revealed that, during cap phase, GABA and GABAB(1) were distributed within the whole acrosomal vesicle but not in the acrosomal granule. GABAB(2) immunoreactivity was observed in the narrow space between the inner acrosomal and nuclear membrane and was limited to the equatorial region of the spermatid head. These results indicate that the GABAergic system might be involved in regulation of spermiogenesis.
Publishing Year
Date Published
2005-07-01
Journal Title
Journal of Andrology
Volume
26
Issue
4
Page
485 - 493
IST-REx-ID

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Kanbara K, Okamoto K, Nomura S, et al. Cellular localization of GABA and GABAB receptor subunit proteins during spermiogenesis in rat testis. Journal of Andrology. 2005;26(4):485-493. doi:10.2164/jandrol.04185
Kanbara, K., Okamoto, K., Nomura, S., Kaneko, T., Shigemoto, R., Azuma, H., … Watanabe, M. (2005). Cellular localization of GABA and GABAB receptor subunit proteins during spermiogenesis in rat testis. Journal of Andrology, 26(4), 485–493. https://doi.org/10.2164/jandrol.04185
Kanbara, Kiyoto, Keiko Okamoto, Sakashi Nomura, Takeshi Kaneko, Ryuichi Shigemoto, Haruhito Azuma, Yoji Katsuoka, and Masahiko Watanabe. “Cellular Localization of GABA and GABAB Receptor Subunit Proteins during Spermiogenesis in Rat Testis.” Journal of Andrology 26, no. 4 (2005): 485–93. https://doi.org/10.2164/jandrol.04185.
K. Kanbara et al., “Cellular localization of GABA and GABAB receptor subunit proteins during spermiogenesis in rat testis,” Journal of Andrology, vol. 26, no. 4, pp. 485–493, 2005.
Kanbara K, Okamoto K, Nomura S, Kaneko T, Shigemoto R, Azuma H, Katsuoka Y, Watanabe M. 2005. Cellular localization of GABA and GABAB receptor subunit proteins during spermiogenesis in rat testis. Journal of Andrology. 26(4), 485–493.
Kanbara, Kiyoto, et al. “Cellular Localization of GABA and GABAB Receptor Subunit Proteins during Spermiogenesis in Rat Testis.” Journal of Andrology, vol. 26, no. 4, American Society of Andrology, 2005, pp. 485–93, doi:10.2164/jandrol.04185.

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