{"date_created":"2018-12-11T11:58:49Z","author":[{"last_name":"Kang","first_name":"Youngnam","full_name":"Kang, Youngnam"},{"last_name":"Notomi","full_name":"Notomi, Takuya","first_name":"Takuya"},{"last_name":"Saito","first_name":"Mitsuru","full_name":"Saito, Mitsuru"},{"first_name":"Wei","full_name":"Zhang, Wei","last_name":"Zhang"},{"last_name":"Shigemoto","first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8761-9444","full_name":"Ryuichi Shigemoto"}],"date_updated":"2021-01-12T06:58:45Z","doi":"10.1523/JNEUROSCI.5641-03.2004","status":"public","issue":"14","publication":"Journal of Neuroscience","quality_controlled":0,"type":"journal_article","title":"Bidirectional interactions between H-channels and Na+-K + pumps in mesencephalic trigeminal neurons","abstract":[{"text":"The Na+-K+ pump current (Ip) and the h-current (Ih) flowing through hyperpolarization-activated channels (h-channels) participate in generating the resting potential. These two currents are thought to be produced independently. We show here bidirectional interactions between Na+-K+ pumps and h-channels in mesencephalic trigeminal neurons. Activation of Ih leads to the generation of two types of ouabain-sensitive Ip with temporal profiles similar to those of instantaneous and slow components of I h, presumably reflecting Na+ transients in a restricted cellular space. Moreover, the Ip activated by instantaneous I h can facilitate the subsequent activation of slow Ih. Such counteractive and cooperative interactions were also disclosed by replacing extracellular Na+ with Li+, which is permeant through h-channels but does not stimulate the Na+-K+ pump as strongly as Na+ ions. These observations indicate that the interactions are bidirectional and mediated by Na+ ions. Also after substitution of extracellular Na+ with Li+, the tail Ih was reduced markedly despite an enhancement of Ih itself, attributable to a negative shift of the reversal potential for I h presumably caused by intracellular accumulation of Li+ ions. This suggests the presence of a microdomain where the interactions can take place. Thus, the bidirectional interactions between Na+-K + pumps and h-channels are likely to be mediated by Na+ microdomain. Consistent with these findings, hyperpolarization-activated and cyclic nucleotide-modulated subunits (HCN1/2) and the Na+-K + pump α3 isoform were colocalized in plasma membrane of mesencephalic trigeminal neurons having numerous spines.","lang":"eng"}],"year":"2004","_id":"2641","month":"04","date_published":"2004-04-07T00:00:00Z","volume":24,"extern":1,"intvolume":"        24","page":"3694 - 3702","publisher":"Society for Neuroscience","publication_status":"published","publist_id":"4257","citation":{"ama":"Kang Y, Notomi T, Saito M, Zhang W, Shigemoto R. Bidirectional interactions between H-channels and Na+-K + pumps in mesencephalic trigeminal neurons. <i>Journal of Neuroscience</i>. 2004;24(14):3694-3702. doi:<a href=\"https://doi.org/10.1523/JNEUROSCI.5641-03.2004\">10.1523/JNEUROSCI.5641-03.2004</a>","chicago":"Kang, Youngnam, Takuya Notomi, Mitsuru Saito, Wei Zhang, and Ryuichi Shigemoto. “Bidirectional Interactions between H-Channels and Na+-K + Pumps in Mesencephalic Trigeminal Neurons.” <i>Journal of Neuroscience</i>. Society for Neuroscience, 2004. <a href=\"https://doi.org/10.1523/JNEUROSCI.5641-03.2004\">https://doi.org/10.1523/JNEUROSCI.5641-03.2004</a>.","mla":"Kang, Youngnam, et al. “Bidirectional Interactions between H-Channels and Na+-K + Pumps in Mesencephalic Trigeminal Neurons.” <i>Journal of Neuroscience</i>, vol. 24, no. 14, Society for Neuroscience, 2004, pp. 3694–702, doi:<a href=\"https://doi.org/10.1523/JNEUROSCI.5641-03.2004\">10.1523/JNEUROSCI.5641-03.2004</a>.","short":"Y. Kang, T. Notomi, M. Saito, W. Zhang, R. Shigemoto, Journal of Neuroscience 24 (2004) 3694–3702.","ista":"Kang Y, Notomi T, Saito M, Zhang W, Shigemoto R. 2004. Bidirectional interactions between H-channels and Na+-K + pumps in mesencephalic trigeminal neurons. Journal of Neuroscience. 24(14), 3694–3702.","ieee":"Y. Kang, T. Notomi, M. Saito, W. Zhang, and R. Shigemoto, “Bidirectional interactions between H-channels and Na+-K + pumps in mesencephalic trigeminal neurons,” <i>Journal of Neuroscience</i>, vol. 24, no. 14. Society for Neuroscience, pp. 3694–3702, 2004.","apa":"Kang, Y., Notomi, T., Saito, M., Zhang, W., &#38; Shigemoto, R. (2004). Bidirectional interactions between H-channels and Na+-K + pumps in mesencephalic trigeminal neurons. <i>Journal of Neuroscience</i>. Society for Neuroscience. <a href=\"https://doi.org/10.1523/JNEUROSCI.5641-03.2004\">https://doi.org/10.1523/JNEUROSCI.5641-03.2004</a>"},"day":"07"}