Bidirectional interactions between H-channels and Na+-K + pumps in mesencephalic trigeminal neurons

Y. Kang, T. Notomi, M. Saito, W. Zhang, R. Shigemoto, Journal of Neuroscience 24 (2004) 3694–3702.

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Journal Article | Published
Author
Kang, Youngnam; Notomi, Takuya; Saito, Mitsuru; Zhang, Wei; Shigemoto, RyuichiIST Austria
Abstract
The Na+-K+ pump current (Ip) and the h-current (Ih) flowing through hyperpolarization-activated channels (h-channels) participate in generating the resting potential. These two currents are thought to be produced independently. We show here bidirectional interactions between Na+-K+ pumps and h-channels in mesencephalic trigeminal neurons. Activation of Ih leads to the generation of two types of ouabain-sensitive Ip with temporal profiles similar to those of instantaneous and slow components of I h, presumably reflecting Na+ transients in a restricted cellular space. Moreover, the Ip activated by instantaneous I h can facilitate the subsequent activation of slow Ih. Such counteractive and cooperative interactions were also disclosed by replacing extracellular Na+ with Li+, which is permeant through h-channels but does not stimulate the Na+-K+ pump as strongly as Na+ ions. These observations indicate that the interactions are bidirectional and mediated by Na+ ions. Also after substitution of extracellular Na+ with Li+, the tail Ih was reduced markedly despite an enhancement of Ih itself, attributable to a negative shift of the reversal potential for I h presumably caused by intracellular accumulation of Li+ ions. This suggests the presence of a microdomain where the interactions can take place. Thus, the bidirectional interactions between Na+-K + pumps and h-channels are likely to be mediated by Na+ microdomain. Consistent with these findings, hyperpolarization-activated and cyclic nucleotide-modulated subunits (HCN1/2) and the Na+-K + pump α3 isoform were colocalized in plasma membrane of mesencephalic trigeminal neurons having numerous spines.
Publishing Year
Date Published
2004-04-07
Journal Title
Journal of Neuroscience
Volume
24
Issue
14
Page
3694 - 3702
IST-REx-ID

Cite this

Kang Y, Notomi T, Saito M, Zhang W, Shigemoto R. Bidirectional interactions between H-channels and Na+-K + pumps in mesencephalic trigeminal neurons. Journal of Neuroscience. 2004;24(14):3694-3702. doi:10.1523/JNEUROSCI.5641-03.2004
Kang, Y., Notomi, T., Saito, M., Zhang, W., & Shigemoto, R. (2004). Bidirectional interactions between H-channels and Na+-K + pumps in mesencephalic trigeminal neurons. Journal of Neuroscience, 24(14), 3694–3702. https://doi.org/10.1523/JNEUROSCI.5641-03.2004
Kang, Youngnam, Takuya Notomi, Mitsuru Saito, Wei Zhang, and Ryuichi Shigemoto. “Bidirectional Interactions between H-Channels and Na+-K + Pumps in Mesencephalic Trigeminal Neurons.” Journal of Neuroscience 24, no. 14 (2004): 3694–3702. https://doi.org/10.1523/JNEUROSCI.5641-03.2004.
Y. Kang, T. Notomi, M. Saito, W. Zhang, and R. Shigemoto, “Bidirectional interactions between H-channels and Na+-K + pumps in mesencephalic trigeminal neurons,” Journal of Neuroscience, vol. 24, no. 14, pp. 3694–3702, 2004.
Kang Y, Notomi T, Saito M, Zhang W, Shigemoto R. 2004. Bidirectional interactions between H-channels and Na+-K + pumps in mesencephalic trigeminal neurons. Journal of Neuroscience. 24(14), 3694–3702.
Kang, Youngnam, et al. “Bidirectional Interactions between H-Channels and Na+-K + Pumps in Mesencephalic Trigeminal Neurons.” Journal of Neuroscience, vol. 24, no. 14, Society for Neuroscience, 2004, pp. 3694–702, doi:10.1523/JNEUROSCI.5641-03.2004.

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