--- res: bibo_abstract: - Cyclic ADP-ribose (cADP-ribose) is a putative second messenger or modulator. However, the role of cADP-ribose in the downstream signals of the metabotropic glutamate receptors (mGluRs) is unclear. Here, we show that glutamate stimulates ADP-ribosyl cyclase activity in rat or mouse crude membranes of retina via group III mGluRs or in superior cervical ganglion via group I mGluRs. The retina of mGluR6-deficient mice showed no increase in the ADP-ribosyl cyclase level in response to glutamate. GTP enhanced the initial rate of basal and glutamate-stimulated cyclase activity. GTP-γ-S also stimulated basal activity. To determine whether the coupling mode of mGluRs to ADP-ribosyl cyclase is a feature common to individual cloned mGluRs, we expressed each mGluR subtype in NG108-15 neuroblastoma x glioma hybrid cells. The glutamate-induced stimulation of the cyclase occurs preferentially in NG108-15 cells over-expressing mGluRs1, 3, 5, and 6. Cells expressing mGluR2 or mGluRs4 and 7 exhibit inhibition or no coupling, respectively. Glutamate-induced activation or inhibition of the cyclase activity was eliminated after pre-treatment with cholera or pertussis toxin, respectively. Thus, the subtype-specific coupling of mGluRs to ADP-ribosyl cyclase via G proteins suggests that some glutamate-evoked neuronal functions are mediated by cADP-ribose.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Haruhiro foaf_name: Higashida, Haruhiro foaf_surname: Higashida - foaf_Person: foaf_givenName: Jia foaf_name: Zhang, Jia-Sheng foaf_surname: Zhang - foaf_Person: foaf_givenName: Sumiko foaf_name: Mochida, Sumiko foaf_surname: Mochida - foaf_Person: foaf_givenName: Xiao foaf_name: Chen, Xiao-Liang foaf_surname: Chen - foaf_Person: foaf_givenName: Yeonsook foaf_name: Shin, Yeonsook foaf_surname: Shin - foaf_Person: foaf_givenName: Mami foaf_name: Noda, Mami foaf_surname: Noda - foaf_Person: foaf_givenName: Kazi foaf_name: Hossain, Kazi Z foaf_surname: Hossain - foaf_Person: foaf_givenName: Naoto foaf_name: Hoshi, Naoto foaf_surname: Hoshi - foaf_Person: foaf_givenName: Minako foaf_name: Hashii, Minako foaf_surname: Hashii - foaf_Person: foaf_givenName: Ryuichi foaf_name: Ryuichi Shigemoto foaf_surname: Shigemoto foaf_workInfoHomepage: http://www.librecat.org/personId=499F3ABC-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-8761-9444 - foaf_Person: foaf_givenName: Shigetada foaf_name: Nakanishi, Shigetada foaf_surname: Nakanishi - foaf_Person: foaf_givenName: Yutaka foaf_name: Fukuda, Yutaka foaf_surname: Fukuda - foaf_Person: foaf_givenName: Shigeru foaf_name: Yokoyama, Shigeru foaf_surname: Yokoyama bibo_doi: 10.1046/j.1471-4159.2003.01751.x bibo_issue: '5' bibo_volume: 85 dct_date: 2003^xs_gYear dct_publisher: Wiley-Blackwell@ dct_title: Subtype-specific coupling with ADP-ribosyl cyclase of metabotropic glutamate receptors in retina, cervical superior ganglion and NG108-15 cells@ ...