{"abstract":[{"lang":"eng","text":"In this investigation, we report identification and characterization of a 95 kDa postsynaptic density protein (PSD-95)/discs-large/ ZO-1 (PDZ) domain-containing protein termed tamalin, also recently named GRP1-associated scaffold protein (GRASP), that interacts with group 1 metabotropic glutamate receptors (mGluRs). The yeast two-hybrid system and in vitro pull-down assays indicated that the PDZ domain-containing, amino-terminal half of tamalin directly binds to the class I PDZ-binding motif of group 1 mGluRs. The C-terminal half of tamalin also bound to cytohesins, the members of guanine nucleotide exchange factors (GEFs) specific for the ADP-ribosylation factor (ARF) family of small GTP-binding proteins. Tamalin mRNA is expressed predominantly in the telencephalic region and highly overlaps with the expression of group 1 mGluR mRNAs. Both tamalin and cytohesin-2 were enriched and codistributed with mGluR1a in postsynaptic membrane fractions. Importantly, recombinant and native mGluR1a/tamalin/cytohesin-2 complexes were coimmunoprecipitated from transfected COS-7 cells and rat brain tissue, respectively. Transfection of tamalin and mutant tamalin lacking a cytohesin-binding domain caused an increase and decrease in cell-surface expression of mGluR1a in COS-7 cells, respectively. Furthermore, adenovirus-mediated expression of tamalin and dominant-negative tamalin facilitated and reduced the neuritic distribution of endogenous mGluR5 in cultured hippocampal neurons, respectively. The results indicate that tamalin plays a key role in the association of group 1 mGluRs with the ARF-specific GEF proteins and contributes to intracellular trafficking and the macromolecular organization of group 1 mGluRs at synapses."}],"pmid":1,"oa_version":"None","date_published":"2002-02-15T00:00:00Z","publist_id":"4285","type":"journal_article","publisher":"Society for Neuroscience","article_type":"original","issue":"4","page":"1280 - 1289","day":"15","intvolume":" 22","publication_identifier":{"issn":["0270-6474"]},"language":[{"iso":"eng"}],"date_created":"2018-12-11T11:58:40Z","title":"Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins","article_processing_charge":"No","status":"public","acknowledgement":"This work was supported in part by research grants from the Ministry of Education, Science and Culture of Japan. We thank Bert Vogelstein for providing adenoviral recombination vectors and Haruhiko Bito for a gift of the enolase promoter and technical advice. We are grateful to Atsushi Nishimune and Satoshi Kaneko for technical advice and Kumlesh K. Dev for careful reading of this manuscript.","publication_status":"published","scopus_import":"1","volume":22,"extern":"1","citation":{"mla":"Kitano, Jun, et al. “Tamalin, a PDZ Domain-Containing Protein, Links a Protein Complex Formation of Group 1 Metabotropic Glutamate Receptors and the Guanine Nucleotide Exchange Factor Cytohesins.” Journal of Neuroscience, vol. 22, no. 4, Society for Neuroscience, 2002, pp. 1280–89, doi:10.1523/JNEUROSCI.22-04-01280.2002.","ista":"Kitano J, Kimura K, Yamazaki Y, Soda T, Shigemoto R, Nakajima Y, Nakanishi S. 2002. Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins. Journal of Neuroscience. 22(4), 1280–1289.","short":"J. Kitano, K. Kimura, Y. Yamazaki, T. Soda, R. Shigemoto, Y. Nakajima, S. Nakanishi, Journal of Neuroscience 22 (2002) 1280–1289.","ieee":"J. Kitano et al., “Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins,” Journal of Neuroscience, vol. 22, no. 4. Society for Neuroscience, pp. 1280–1289, 2002.","apa":"Kitano, J., Kimura, K., Yamazaki, Y., Soda, T., Shigemoto, R., Nakajima, Y., & Nakanishi, S. (2002). Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins. Journal of Neuroscience. Society for Neuroscience. https://doi.org/10.1523/JNEUROSCI.22-04-01280.2002","ama":"Kitano J, Kimura K, Yamazaki Y, et al. Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins. Journal of Neuroscience. 2002;22(4):1280-1289. doi:10.1523/JNEUROSCI.22-04-01280.2002","chicago":"Kitano, Jun, Kouji Kimura, Yoshimitsu Yamazaki, Takeshi Soda, Ryuichi Shigemoto, Yoshiaki Nakajima, and Shigetada Nakanishi. “Tamalin, a PDZ Domain-Containing Protein, Links a Protein Complex Formation of Group 1 Metabotropic Glutamate Receptors and the Guanine Nucleotide Exchange Factor Cytohesins.” Journal of Neuroscience. Society for Neuroscience, 2002. https://doi.org/10.1523/JNEUROSCI.22-04-01280.2002."},"month":"02","publication":"Journal of Neuroscience","_id":"2613","year":"2002","doi":"10.1523/JNEUROSCI.22-04-01280.2002","quality_controlled":"1","author":[{"full_name":"Kitano, Jun","last_name":"Kitano","first_name":"Jun"},{"last_name":"Kimura","first_name":"Kouji","full_name":"Kimura, Kouji"},{"last_name":"Yamazaki","first_name":"Yoshimitsu","full_name":"Yamazaki, Yoshimitsu"},{"first_name":"Takeshi","last_name":"Soda","full_name":"Soda, Takeshi"},{"full_name":"Shigemoto, Ryuichi","first_name":"Ryuichi","orcid":"0000-0001-8761-9444","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto"},{"first_name":"Yoshiaki","last_name":"Nakajima","full_name":"Nakajima, Yoshiaki"},{"last_name":"Nakanishi","first_name":"Shigetada","full_name":"Nakanishi, Shigetada"}],"external_id":{"pmid":["11850456"]},"date_updated":"2023-07-25T11:34:46Z","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17"}