---
_id: '2611'
abstract:
- lang: eng
text: Research using animal models of neuropathic pain has revealed sympathetic
sprouting onto dorsal root ganglion cells. More recently, sensory fibre sprouting
onto dorsal root ganglion cells has also been observed. Previous work in our laboratory
demonstrated persistent sympathetic fibre sprouting in the skin of the rat lower
lip following sensory denervation of this region. Therefore, we applied immunocytochemistry
to determine the effects of sympathectomies on the terminal fields of sensory
fibres. The superior cervical ganglia were removed bilaterally and the effects
on the innervation of the skin of the rat lower lip were observed 1, 2, 3, 4,
6 and 8 weeks post-surgery. Substance P and dopamine-β-hydroxylase immunoreactivities
were used to identify a subset of sensory and sympathetic fibres, respectively.
We also assessed neurokinin-1 receptor immunoreactivity. Quantitative data was
obtained with the aid of an image analysis system. In controls, the epidermis
and upper dermis were innervated by substance P-immunoreactive fibres only and
upper dermal blood vessels possessed the highest density of neurokinin-1 receptor
immunoreactivity. Blood vessels in the lower dermis were innervated by both substance
P- and dopamine-β-hydroxylase-immunoreactive fibres. Following sympathectomies,
substance P-immunoreactive fibres in the epidermis and upper dermis were more
intensely labelled only 1 and 2 weeks post-surgery when compared to sham controls.
The length of substance P-immunoreactive fibres in this region was also increased
only on the second week. Neurokinin-1 receptor immunoreactivity in the upper dermis
was slightly decreased 1 and 2 weeks post-surgery. In the lower dermis, substance
P-immunoreactive fibres associated with blood vessels were more intensely labelled
only 1 and 2 weeks post-surgery, and at all post-surgical time points studied,
blood vessels in this region were devoid of dopamine-β-hydroxylase-immunoreactive
fibres. The length of substance P-immunoreactive fibres was increased from the
first to the third week post-surgery in the lower dermis. These results indicate
that sympathectomies lead to transient changes in substance P-immunoreactive fibre
innervation and neurokinin-1 receptor expression in rat lower lip skin. The effects
are most prominent in the lower dermis probably due to a greater local concentration
of nerve growth factor in this region. The plasticity of the interactions between
sensory and sympathetic fibres may prove important in the regulation of skin microcirculation
and in the generation of painful sensations under normal conditions or following
peripheral nerve injuries.
acknowledgement: 'The work contained in this manuscript was sponsored by the Canadian
MRC, Grants # MT-12170 and MoP-38093. The authors would like to thank Sylvain Cote
for technical assistance and Sid Parkinson for editorial assistance.'
article_processing_charge: No
article_type: original
author:
- first_name: Isabella
full_name: Ruocco, Isabella
last_name: Ruocco
- first_name: Augusto
full_name: Cuello, Augusto
last_name: Cuello
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Alfredo
full_name: Ribeiro Da Silva, Alfredo
last_name: Ribeiro Da Silva
citation:
ama: Ruocco I, Cuello A, Shigemoto R, Ribeiro Da Silva A. Sympathectomies lead to
transient substance P-immunoreactive sensory fibre plasticity in the rat skin.
Neuroscience. 2001;108(1):157-166. doi:10.1016/S0306-4522(01)00158-0
apa: Ruocco, I., Cuello, A., Shigemoto, R., & Ribeiro Da Silva, A. (2001). Sympathectomies
lead to transient substance P-immunoreactive sensory fibre plasticity in the rat
skin. Neuroscience. Elsevier. https://doi.org/10.1016/S0306-4522(01)00158-0
chicago: Ruocco, Isabella, Augusto Cuello, Ryuichi Shigemoto, and Alfredo Ribeiro
Da Silva. “Sympathectomies Lead to Transient Substance P-Immunoreactive Sensory
Fibre Plasticity in the Rat Skin.” Neuroscience. Elsevier, 2001. https://doi.org/10.1016/S0306-4522(01)00158-0.
ieee: I. Ruocco, A. Cuello, R. Shigemoto, and A. Ribeiro Da Silva, “Sympathectomies
lead to transient substance P-immunoreactive sensory fibre plasticity in the rat
skin,” Neuroscience, vol. 108, no. 1. Elsevier, pp. 157–166, 2001.
ista: Ruocco I, Cuello A, Shigemoto R, Ribeiro Da Silva A. 2001. Sympathectomies
lead to transient substance P-immunoreactive sensory fibre plasticity in the rat
skin. Neuroscience. 108(1), 157–166.
mla: Ruocco, Isabella, et al. “Sympathectomies Lead to Transient Substance P-Immunoreactive
Sensory Fibre Plasticity in the Rat Skin.” Neuroscience, vol. 108, no.
1, Elsevier, 2001, pp. 157–66, doi:10.1016/S0306-4522(01)00158-0.
short: I. Ruocco, A. Cuello, R. Shigemoto, A. Ribeiro Da Silva, Neuroscience 108
(2001) 157–166.
date_created: 2018-12-11T11:58:40Z
date_published: 2001-12-05T00:00:00Z
date_updated: 2023-05-22T12:15:44Z
day: '05'
doi: 10.1016/S0306-4522(01)00158-0
extern: '1'
external_id:
pmid:
- '11738139'
intvolume: ' 108'
issue: '1'
language:
- iso: eng
month: '12'
oa_version: None
page: 157 - 166
pmid: 1
publication: Neuroscience
publication_identifier:
issn:
- 0306-4522
publication_status: published
publisher: Elsevier
publist_id: '4286'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Sympathectomies lead to transient substance P-immunoreactive sensory fibre
plasticity in the rat skin
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 108
year: '2001'
...