{"status":"public","day":"15","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6762269/"}],"publication_identifier":{"issn":["0270-6474"]},"date_published":"2001-11-15T00:00:00Z","external_id":{"pmid":["11698585"]},"quality_controlled":"1","publication":"Journal of Neuroscience","issue":"22","language":[{"iso":"eng"}],"article_processing_charge":"No","publisher":"Society for Neuroscience","abstract":[{"text":"To study the role of mGlu7 receptors (mGluR7), we used homologous recombination to generate mice lacking this metabotropic receptor subtype (mGluR7 -/-). After the serendipitous discovery of a sensory stimulus-evoked epileptic phenotype, we tested two convulsant drugs, pentylenetetrazole (PTZ) and bicuculline. In animals aged 12 weeks and older, subthreshold doses of these drugs induced seizures in mGluR7 -/-, but not in mGluR7 +/-, mice. PTZ-induced seizures were inhibited by three standard anticonvulsant drugs, but not by the group III selective mGluR agonist (R,S)-4-phosphonophenylglycine (PPG). Consistent with the lack of signs of epileptic activity in the absence of specific stimuli, mGluR7 -/- mice showed no major changes in synaptic properties in two slice preparations. However, slightly increased excitability was evident in hippocampal slices. In addition, there was slower recovery from frequency facilitation in cortical slices, suggesting a role for mGluR7 as a frequency-dependent regulator in presynaptic terminals. Our findings suggest that mGluR7 receptors have a unique role in regulating neuronal excitability and that these receptors may be a novel target for the development of anticonvulsant drugs.","lang":"eng"}],"publist_id":"4288","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","scopus_import":"1","doi":"10.1523/JNEUROSCI.21-22-08734.2001","citation":{"apa":"Sansig, G., Bushell, T., Clarke, V., Rozov, A., Burnashev, N., Portet, C., … Van Der Putten, H. (2001). Increased seizure susceptibility in mice lacking metabotropic glutamate receptor 7. <i>Journal of Neuroscience</i>. Society for Neuroscience. <a href=\"https://doi.org/10.1523/JNEUROSCI.21-22-08734.2001\">https://doi.org/10.1523/JNEUROSCI.21-22-08734.2001</a>","short":"G. Sansig, T. Bushell, V. Clarke, A. Rozov, N. Burnashev, C. Portet, F. Gasparini, M. Schmutz, K. Klebs, R. Shigemoto, P. Flor, R. Kühn, T. Knoepfel, M. Schroeder, D. Hampson, V. Collett, C. Zhang, R. Duvoisin, G. Collingridge, H. Van Der Putten, Journal of Neuroscience 21 (2001) 8734–8745.","mla":"Sansig, Gilles, et al. “Increased Seizure Susceptibility in Mice Lacking Metabotropic Glutamate Receptor 7.” <i>Journal of Neuroscience</i>, vol. 21, no. 22, Society for Neuroscience, 2001, pp. 8734–45, doi:<a href=\"https://doi.org/10.1523/JNEUROSCI.21-22-08734.2001\">10.1523/JNEUROSCI.21-22-08734.2001</a>.","ista":"Sansig G, Bushell T, Clarke V, Rozov A, Burnashev N, Portet C, Gasparini F, Schmutz M, Klebs K, Shigemoto R, Flor P, Kühn R, Knoepfel T, Schroeder M, Hampson D, Collett V, Zhang C, Duvoisin R, Collingridge G, Van Der Putten H. 2001. Increased seizure susceptibility in mice lacking metabotropic glutamate receptor 7. Journal of Neuroscience. 21(22), 8734–8745.","ieee":"G. Sansig <i>et al.</i>, “Increased seizure susceptibility in mice lacking metabotropic glutamate receptor 7,” <i>Journal of Neuroscience</i>, vol. 21, no. 22. Society for Neuroscience, pp. 8734–8745, 2001.","ama":"Sansig G, Bushell T, Clarke V, et al. Increased seizure susceptibility in mice lacking metabotropic glutamate receptor 7. <i>Journal of Neuroscience</i>. 2001;21(22):8734-8745. doi:<a href=\"https://doi.org/10.1523/JNEUROSCI.21-22-08734.2001\">10.1523/JNEUROSCI.21-22-08734.2001</a>","chicago":"Sansig, Gilles, Trevor Bushell, Vernon Clarke, Andrei Rozov, Nail Burnashev, Chantal Portet, Fabrizio Gasparini, et al. “Increased Seizure Susceptibility in Mice Lacking Metabotropic Glutamate Receptor 7.” <i>Journal of Neuroscience</i>. Society for Neuroscience, 2001. <a href=\"https://doi.org/10.1523/JNEUROSCI.21-22-08734.2001\">https://doi.org/10.1523/JNEUROSCI.21-22-08734.2001</a>."},"type":"journal_article","year":"2001","author":[{"full_name":"Sansig, Gilles","last_name":"Sansig","first_name":"Gilles"},{"last_name":"Bushell","full_name":"Bushell, Trevor","first_name":"Trevor"},{"first_name":"Vernon","last_name":"Clarke","full_name":"Clarke, Vernon"},{"last_name":"Rozov","full_name":"Rozov, Andrei","first_name":"Andrei"},{"first_name":"Nail","full_name":"Burnashev, Nail","last_name":"Burnashev"},{"last_name":"Portet","full_name":"Portet, Chantal","first_name":"Chantal"},{"full_name":"Gasparini, Fabrizio","last_name":"Gasparini","first_name":"Fabrizio"},{"first_name":"Markus","full_name":"Schmutz, Markus","last_name":"Schmutz"},{"first_name":"Klaus","full_name":"Klebs, Klaus","last_name":"Klebs"},{"first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto","full_name":"Shigemoto, Ryuichi","orcid":"0000-0001-8761-9444"},{"first_name":"Peter","full_name":"Flor, Peter","last_name":"Flor"},{"first_name":"Rainer","last_name":"Kühn","full_name":"Kühn, Rainer"},{"last_name":"Knoepfel","full_name":"Knoepfel, Thomas","first_name":"Thomas"},{"full_name":"Schroeder, Markus","last_name":"Schroeder","first_name":"Markus"},{"first_name":"David","last_name":"Hampson","full_name":"Hampson, David"},{"full_name":"Collett, Valerie","last_name":"Collett","first_name":"Valerie"},{"full_name":"Zhang, Congxiao","last_name":"Zhang","first_name":"Congxiao"},{"last_name":"Duvoisin","full_name":"Duvoisin, Robert","first_name":"Robert"},{"first_name":"Graham","last_name":"Collingridge","full_name":"Collingridge, Graham"},{"first_name":"Herman","full_name":"Van Der Putten, Herman","last_name":"Van Der Putten"}],"title":"Increased seizure susceptibility in mice lacking metabotropic glutamate receptor 7","_id":"2610","volume":21,"date_updated":"2023-05-24T08:47:53Z","month":"11","page":"8734 - 8745","date_created":"2018-12-11T11:58:39Z","extern":"1","intvolume":"        21","acknowledgement":"This work was supported in part by the Biotechnology and Biological Sciences Research Council and Medical Research Council (UK). We thank Doris Ruegg for sequencing, Gemma Texido and Klaus Rajewsky for pTV-0 DNA, J.-F. Pin for mGluR8 cDNA, K. von Figura for E14 ES cells, Pedro Grandes for histological examination of brain sections, Christoph Wiessner for help with plots and statistics, Valerie Schuler for help with Western blots, and the team of the Novartis special strain breeding facility for their support.","article_type":"original","publication_status":"published","pmid":1,"oa":1,"oa_version":"Published Version"}