---
_id: '2607'
abstract:
- lang: eng
text: Alternative splicing in the mGluR5 gene generates two different receptor isoforms,
of which expression is developmentally regulated. However, little is known about
the functional significance of mGluR5 splice variants. We have examined the functional
coupling, subcellular targeting, and effect on neuronal differentiation of epitope-tagged
mGluR5 isoforms by expression in neuroblastoma NG108-15 cells. We found that both
mGluR5 splice variants give rise to comparable [Ca2+]i transients and have similar
pharmacological profile. Tagged receptors were shown by immunofluorescence to
be inserted in the plasma membrane. In undifferentiated cells the subcellular
localization of the two mGluR5 isoforms was partially segregated, whereas in differentiated
cells the labeling largely redistributed to the newly formed neurites. Interestingly,
we demonstrate that mGluR5 splice variants dramatically influence the formation
and maturation of neurites; mGluR5a hinders the acquisition of mature neuronal
traits and mGluR5b fosters the elaboration and extension of neurites. These effects
are partly inhibited by MPEP.
acknowledgement: "The authors thank: Dr. J. M. Rimland and M. T. Scupoli for their
technical help with X. oocytes recordings and FAC sorting, respectively; Dr. Y.
Dalezios for helping with the statistical analyses; and Dr. G. Varani for helping
with the analyses of mRNA and genomic sequences. We are also grateful to Professor
F. Benfenati, Dr. F. Conquet, Dr. Rafael Lujan, Dr. J. McIlhinney, Professor P.
Somogyi, Dr. J. H. Xuereb, and Dr. M. Zoli for careful reading of the manuscript\r\nand
helpful suggestions. R.S. is supported by the Laboratory of Cerebral Structure,
National Institute for Physiological Sciences, Myodaiji, Okazaki 444-8585, CREST
Japan Science and Technology Corporation, Japan."
article_processing_charge: No
article_type: original
author:
- first_name: Silvia
full_name: Mion, Silvia
last_name: Mion
- first_name: Corrado
full_name: Corti, Corrado
last_name: Corti
- first_name: Akio
full_name: Neki, Akio
last_name: Neki
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Mauro
full_name: Corsi, Mauro
last_name: Corsi
- first_name: Guido
full_name: Fumagalli, Guido
last_name: Fumagalli
- first_name: Francesco
full_name: Ferraguti, Francesco
last_name: Ferraguti
citation:
ama: Mion S, Corti C, Neki A, et al. Bidirectional regulation of neurite elaboration
by alternatively spliced metabotropic glutamate receptor 5 (mGluR5) isoforms.
Molecular and Cellular Neuroscience. 2001;17(6):957-972. doi:10.1006/mcne.2001.0993
apa: Mion, S., Corti, C., Neki, A., Shigemoto, R., Corsi, M., Fumagalli, G., &
Ferraguti, F. (2001). Bidirectional regulation of neurite elaboration by alternatively
spliced metabotropic glutamate receptor 5 (mGluR5) isoforms. Molecular and
Cellular Neuroscience. Academic Press. https://doi.org/10.1006/mcne.2001.0993
chicago: Mion, Silvia, Corrado Corti, Akio Neki, Ryuichi Shigemoto, Mauro Corsi,
Guido Fumagalli, and Francesco Ferraguti. “Bidirectional Regulation of Neurite
Elaboration by Alternatively Spliced Metabotropic Glutamate Receptor 5 (MGluR5)
Isoforms.” Molecular and Cellular Neuroscience. Academic Press, 2001. https://doi.org/10.1006/mcne.2001.0993.
ieee: S. Mion et al., “Bidirectional regulation of neurite elaboration by
alternatively spliced metabotropic glutamate receptor 5 (mGluR5) isoforms,” Molecular
and Cellular Neuroscience, vol. 17, no. 6. Academic Press, pp. 957–972, 2001.
ista: Mion S, Corti C, Neki A, Shigemoto R, Corsi M, Fumagalli G, Ferraguti F. 2001.
Bidirectional regulation of neurite elaboration by alternatively spliced metabotropic
glutamate receptor 5 (mGluR5) isoforms. Molecular and Cellular Neuroscience. 17(6),
957–972.
mla: Mion, Silvia, et al. “Bidirectional Regulation of Neurite Elaboration by Alternatively
Spliced Metabotropic Glutamate Receptor 5 (MGluR5) Isoforms.” Molecular and
Cellular Neuroscience, vol. 17, no. 6, Academic Press, 2001, pp. 957–72, doi:10.1006/mcne.2001.0993.
short: S. Mion, C. Corti, A. Neki, R. Shigemoto, M. Corsi, G. Fumagalli, F. Ferraguti,
Molecular and Cellular Neuroscience 17 (2001) 957–972.
date_created: 2018-12-11T11:58:38Z
date_published: 2001-06-01T00:00:00Z
date_updated: 2023-05-24T09:34:13Z
day: '01'
doi: 10.1006/mcne.2001.0993
extern: '1'
external_id:
pmid:
- '11414786'
intvolume: ' 17'
issue: '6'
language:
- iso: eng
month: '06'
oa_version: None
page: 957 - 972
pmid: 1
publication: Molecular and Cellular Neuroscience
publication_identifier:
issn:
- 1044-7431
publication_status: published
publisher: Academic Press
publist_id: '4291'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Bidirectional regulation of neurite elaboration by alternatively spliced metabotropic
glutamate receptor 5 (mGluR5) isoforms
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 17
year: '2001'
...