--- _id: '2607' abstract: - lang: eng text: Alternative splicing in the mGluR5 gene generates two different receptor isoforms, of which expression is developmentally regulated. However, little is known about the functional significance of mGluR5 splice variants. We have examined the functional coupling, subcellular targeting, and effect on neuronal differentiation of epitope-tagged mGluR5 isoforms by expression in neuroblastoma NG108-15 cells. We found that both mGluR5 splice variants give rise to comparable [Ca2+]i transients and have similar pharmacological profile. Tagged receptors were shown by immunofluorescence to be inserted in the plasma membrane. In undifferentiated cells the subcellular localization of the two mGluR5 isoforms was partially segregated, whereas in differentiated cells the labeling largely redistributed to the newly formed neurites. Interestingly, we demonstrate that mGluR5 splice variants dramatically influence the formation and maturation of neurites; mGluR5a hinders the acquisition of mature neuronal traits and mGluR5b fosters the elaboration and extension of neurites. These effects are partly inhibited by MPEP. acknowledgement: "The authors thank: Dr. J. M. Rimland and M. T. Scupoli for their technical help with X. oocytes recordings and FAC sorting, respectively; Dr. Y. Dalezios for helping with the statistical analyses; and Dr. G. Varani for helping with the analyses of mRNA and genomic sequences. We are also grateful to Professor F. Benfenati, Dr. F. Conquet, Dr. Rafael Lujan, Dr. J. McIlhinney, Professor P. Somogyi, Dr. J. H. Xuereb, and Dr. M. Zoli for careful reading of the manuscript\r\nand helpful suggestions. R.S. is supported by the Laboratory of Cerebral Structure, National Institute for Physiological Sciences, Myodaiji, Okazaki 444-8585, CREST Japan Science and Technology Corporation, Japan." article_processing_charge: No article_type: original author: - first_name: Silvia full_name: Mion, Silvia last_name: Mion - first_name: Corrado full_name: Corti, Corrado last_name: Corti - first_name: Akio full_name: Neki, Akio last_name: Neki - first_name: Ryuichi full_name: Shigemoto, Ryuichi id: 499F3ABC-F248-11E8-B48F-1D18A9856A87 last_name: Shigemoto orcid: 0000-0001-8761-9444 - first_name: Mauro full_name: Corsi, Mauro last_name: Corsi - first_name: Guido full_name: Fumagalli, Guido last_name: Fumagalli - first_name: Francesco full_name: Ferraguti, Francesco last_name: Ferraguti citation: ama: Mion S, Corti C, Neki A, et al. Bidirectional regulation of neurite elaboration by alternatively spliced metabotropic glutamate receptor 5 (mGluR5) isoforms. Molecular and Cellular Neuroscience. 2001;17(6):957-972. doi:10.1006/mcne.2001.0993 apa: Mion, S., Corti, C., Neki, A., Shigemoto, R., Corsi, M., Fumagalli, G., & Ferraguti, F. (2001). Bidirectional regulation of neurite elaboration by alternatively spliced metabotropic glutamate receptor 5 (mGluR5) isoforms. Molecular and Cellular Neuroscience. Academic Press. https://doi.org/10.1006/mcne.2001.0993 chicago: Mion, Silvia, Corrado Corti, Akio Neki, Ryuichi Shigemoto, Mauro Corsi, Guido Fumagalli, and Francesco Ferraguti. “Bidirectional Regulation of Neurite Elaboration by Alternatively Spliced Metabotropic Glutamate Receptor 5 (MGluR5) Isoforms.” Molecular and Cellular Neuroscience. Academic Press, 2001. https://doi.org/10.1006/mcne.2001.0993. ieee: S. Mion et al., “Bidirectional regulation of neurite elaboration by alternatively spliced metabotropic glutamate receptor 5 (mGluR5) isoforms,” Molecular and Cellular Neuroscience, vol. 17, no. 6. Academic Press, pp. 957–972, 2001. ista: Mion S, Corti C, Neki A, Shigemoto R, Corsi M, Fumagalli G, Ferraguti F. 2001. Bidirectional regulation of neurite elaboration by alternatively spliced metabotropic glutamate receptor 5 (mGluR5) isoforms. Molecular and Cellular Neuroscience. 17(6), 957–972. mla: Mion, Silvia, et al. “Bidirectional Regulation of Neurite Elaboration by Alternatively Spliced Metabotropic Glutamate Receptor 5 (MGluR5) Isoforms.” Molecular and Cellular Neuroscience, vol. 17, no. 6, Academic Press, 2001, pp. 957–72, doi:10.1006/mcne.2001.0993. short: S. Mion, C. Corti, A. Neki, R. Shigemoto, M. Corsi, G. Fumagalli, F. Ferraguti, Molecular and Cellular Neuroscience 17 (2001) 957–972. date_created: 2018-12-11T11:58:38Z date_published: 2001-06-01T00:00:00Z date_updated: 2023-05-24T09:34:13Z day: '01' doi: 10.1006/mcne.2001.0993 extern: '1' external_id: pmid: - '11414786' intvolume: ' 17' issue: '6' language: - iso: eng month: '06' oa_version: None page: 957 - 972 pmid: 1 publication: Molecular and Cellular Neuroscience publication_identifier: issn: - 1044-7431 publication_status: published publisher: Academic Press publist_id: '4291' quality_controlled: '1' scopus_import: '1' status: public title: Bidirectional regulation of neurite elaboration by alternatively spliced metabotropic glutamate receptor 5 (mGluR5) isoforms type: journal_article user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17 volume: 17 year: '2001' ...