Bidirectional regulation of neurite elaboration by alternatively spliced metabotropic glutamate receptor 5 (mGluR5) isoforms

S. Mion, C. Corti, A. Neki, R. Shigemoto, M. Corsi, G. Fumagalli, F. Ferraguti, Molecular and Cellular Neuroscience 17 (2001) 957–972.

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Journal Article | Published
Author
Mion, Silvia; Corti, Corrado; Neki, Akio; Shigemoto, RyuichiIST Austria ; Corsi, Mauro A; Fumagalli, Guido F; Ferraguti, Francesco
Abstract
Alternative splicing in the mGluR5 gene generates two different receptor isoforms, of which expression is developmentally regulated. However, little is known about the functional significance of mGluR5 splice variants. We have examined the functional coupling, subcellular targeting, and effect on neuronal differentiation of epitope-tagged mGluR5 isoforms by expression in neuroblastoma NG108-15 cells. We found that both mGluR5 splice variants give rise to comparable [Ca2+]i transients and have similar pharmacological profile. Tagged receptors were shown by immunofluorescence to be inserted in the plasma membrane. In undifferentiated cells the subcellular localization of the two mGluR5 isoforms was partially segregated, whereas in differentiated cells the labeling largely redistributed to the newly formed neurites. Interestingly, we demonstrate that mGluR5 splice variants dramatically influence the formation and maturation of neurites; mGluR5a hinders the acquisition of mature neuronal traits and mGluR5b fosters the elaboration and extension of neurites. These effects are partly inhibited by MPEP.
Publishing Year
Date Published
2001-06-01
Journal Title
Molecular and Cellular Neuroscience
Volume
17
Issue
6
Page
957 - 972
IST-REx-ID

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Mion S, Corti C, Neki A, et al. Bidirectional regulation of neurite elaboration by alternatively spliced metabotropic glutamate receptor 5 (mGluR5) isoforms. Molecular and Cellular Neuroscience. 2001;17(6):957-972. doi:10.1006/mcne.2001.0993
Mion, S., Corti, C., Neki, A., Shigemoto, R., Corsi, M., Fumagalli, G., & Ferraguti, F. (2001). Bidirectional regulation of neurite elaboration by alternatively spliced metabotropic glutamate receptor 5 (mGluR5) isoforms. Molecular and Cellular Neuroscience, 17(6), 957–972. https://doi.org/10.1006/mcne.2001.0993
Mion, Silvia, Corrado Corti, Akio Neki, Ryuichi Shigemoto, Mauro Corsi, Guido Fumagalli, and Francesco Ferraguti. “Bidirectional Regulation of Neurite Elaboration by Alternatively Spliced Metabotropic Glutamate Receptor 5 (MGluR5) Isoforms.” Molecular and Cellular Neuroscience 17, no. 6 (2001): 957–72. https://doi.org/10.1006/mcne.2001.0993.
S. Mion et al., “Bidirectional regulation of neurite elaboration by alternatively spliced metabotropic glutamate receptor 5 (mGluR5) isoforms,” Molecular and Cellular Neuroscience, vol. 17, no. 6, pp. 957–972, 2001.
Mion S, Corti C, Neki A, Shigemoto R, Corsi M, Fumagalli G, Ferraguti F. 2001. Bidirectional regulation of neurite elaboration by alternatively spliced metabotropic glutamate receptor 5 (mGluR5) isoforms. Molecular and Cellular Neuroscience. 17(6), 957–972.
Mion, Silvia, et al. “Bidirectional Regulation of Neurite Elaboration by Alternatively Spliced Metabotropic Glutamate Receptor 5 (MGluR5) Isoforms.” Molecular and Cellular Neuroscience, vol. 17, no. 6, Academic Press, 2001, pp. 957–72, doi:10.1006/mcne.2001.0993.

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