---
_id: '2586'
abstract:
- lang: eng
text: The role of inhibitory Golgi cells in cerebellar function was investigated
by selectively ablating Golgi cells expressing human interleukin-2 receptor α
subunit in transgenic mice, using the immunotoxin- mediated cell targeting technique.
Golgi cell disruption caused severe acute motor disorders. These mice showed gradual
recovery but retained a continuing inability to perform compound movements. Optical
and electrical recordings combined with immunocytological analysis indicated that
elimination of Golgi cells not only reduces GABA-mediated inhibition but also
attenuates functional NMDA receptors in granule cells. These results demonstrate
that synaptic integration involving both GABA inhibition and NMDA receptor activation
is essential for compound motor coordination. Furthermore, this integration can
adapt after Golgi cell elimination so as not to evoke overexcitation by the reduction
of NMDA receptors.
acknowledgement: "We thank Kumlesh K Dev for careful reading of this manuscript, Peter
Somogyi and Hirohide Sawada for invaluable advice, and Akira Uesugi for photographic
assistance. This work was supported in part by research grants from the Ministry
of Education, Science and Culture of Japan. the Sankyo Foundation. the Yamanouchi
Founda-tion. the Biomolecular Engineering Research Institute, CREST and the International
Resource Program of the National Cancer Institute. \r\n"
article_processing_charge: No
article_type: original
author:
- first_name: Dai
full_name: Watanabe, Dai
last_name: Watanabe
- first_name: Hitoshi
full_name: Inokawa, Hitoshi
last_name: Inokawa
- first_name: Kouichi
full_name: Hashimoto, Kouichi
last_name: Hashimoto
- first_name: Noboru
full_name: Suzuki, Noboru
last_name: Suzuki
- first_name: Masanobu
full_name: Kano, Masanobu
last_name: Kano
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Tomoo
full_name: Hirano, Tomoo
last_name: Hirano
- first_name: Keisuke
full_name: Toyama, Keisuke
last_name: Toyama
- first_name: Satoshi
full_name: Kaneko, Satoshi
last_name: Kaneko
- first_name: Mineto
full_name: Yokoi, Mineto
last_name: Yokoi
- first_name: Koki
full_name: Moriyoshi, Koki
last_name: Moriyoshi
- first_name: Misao
full_name: Suzuki, Misao
last_name: Suzuki
- first_name: Kazuto
full_name: Kobayashi, Kazuto
last_name: Kobayashi
- first_name: Toshiharu
full_name: Nagatsu, Toshiharu
last_name: Nagatsu
- first_name: Robert
full_name: Kreitman, Robert
last_name: Kreitman
- first_name: Ira
full_name: Pastan, Ira
last_name: Pastan
- first_name: Shigetada
full_name: Nakanishi, Shigetada
last_name: Nakanishi
citation:
ama: Watanabe D, Inokawa H, Hashimoto K, et al. Ablation of cerebellar Golgi cells
disrupts synaptic integration involving GABA inhibition and NMDA receptor activation
in motor coordination. Cell. 1998;95(1):17-27. doi:10.1016/S0092-8674(00)81779-1
apa: Watanabe, D., Inokawa, H., Hashimoto, K., Suzuki, N., Kano, M., Shigemoto,
R., … Nakanishi, S. (1998). Ablation of cerebellar Golgi cells disrupts synaptic
integration involving GABA inhibition and NMDA receptor activation in motor coordination.
Cell. Cell Press. https://doi.org/10.1016/S0092-8674(00)81779-1
chicago: Watanabe, Dai, Hitoshi Inokawa, Kouichi Hashimoto, Noboru Suzuki, Masanobu
Kano, Ryuichi Shigemoto, Tomoo Hirano, et al. “Ablation of Cerebellar Golgi Cells
Disrupts Synaptic Integration Involving GABA Inhibition and NMDA Receptor Activation
in Motor Coordination.” Cell. Cell Press, 1998. https://doi.org/10.1016/S0092-8674(00)81779-1.
ieee: D. Watanabe et al., “Ablation of cerebellar Golgi cells disrupts synaptic
integration involving GABA inhibition and NMDA receptor activation in motor coordination,”
Cell, vol. 95, no. 1. Cell Press, pp. 17–27, 1998.
ista: Watanabe D, Inokawa H, Hashimoto K, Suzuki N, Kano M, Shigemoto R, Hirano
T, Toyama K, Kaneko S, Yokoi M, Moriyoshi K, Suzuki M, Kobayashi K, Nagatsu T,
Kreitman R, Pastan I, Nakanishi S. 1998. Ablation of cerebellar Golgi cells disrupts
synaptic integration involving GABA inhibition and NMDA receptor activation in
motor coordination. Cell. 95(1), 17–27.
mla: Watanabe, Dai, et al. “Ablation of Cerebellar Golgi Cells Disrupts Synaptic
Integration Involving GABA Inhibition and NMDA Receptor Activation in Motor Coordination.”
Cell, vol. 95, no. 1, Cell Press, 1998, pp. 17–27, doi:10.1016/S0092-8674(00)81779-1.
short: D. Watanabe, H. Inokawa, K. Hashimoto, N. Suzuki, M. Kano, R. Shigemoto,
T. Hirano, K. Toyama, S. Kaneko, M. Yokoi, K. Moriyoshi, M. Suzuki, K. Kobayashi,
T. Nagatsu, R. Kreitman, I. Pastan, S. Nakanishi, Cell 95 (1998) 17–27.
date_created: 2018-12-11T11:58:32Z
date_published: 1998-10-02T00:00:00Z
date_updated: 2022-08-31T13:46:20Z
day: '02'
doi: 10.1016/S0092-8674(00)81779-1
extern: '1'
external_id:
pmid:
- '9778244 '
intvolume: ' 95'
issue: '1'
language:
- iso: eng
month: '10'
oa_version: None
page: 17 - 27
pmid: 1
publication: Cell
publication_identifier:
issn:
- 0092-8674
publication_status: published
publisher: Cell Press
publist_id: '4312'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Ablation of cerebellar Golgi cells disrupts synaptic integration involving
GABA inhibition and NMDA receptor activation in motor coordination
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 95
year: '1998'
...