--- _id: '2586' abstract: - lang: eng text: The role of inhibitory Golgi cells in cerebellar function was investigated by selectively ablating Golgi cells expressing human interleukin-2 receptor α subunit in transgenic mice, using the immunotoxin- mediated cell targeting technique. Golgi cell disruption caused severe acute motor disorders. These mice showed gradual recovery but retained a continuing inability to perform compound movements. Optical and electrical recordings combined with immunocytological analysis indicated that elimination of Golgi cells not only reduces GABA-mediated inhibition but also attenuates functional NMDA receptors in granule cells. These results demonstrate that synaptic integration involving both GABA inhibition and NMDA receptor activation is essential for compound motor coordination. Furthermore, this integration can adapt after Golgi cell elimination so as not to evoke overexcitation by the reduction of NMDA receptors. acknowledgement: "We thank Kumlesh K Dev for careful reading of this manuscript, Peter Somogyi and Hirohide Sawada for invaluable advice, and Akira Uesugi for photographic assistance. This work was supported in part by research grants from the Ministry of Education, Science and Culture of Japan. the Sankyo Foundation. the Yamanouchi Founda-tion. the Biomolecular Engineering Research Institute, CREST and the International Resource Program of the National Cancer Institute. \r\n" article_processing_charge: No article_type: original author: - first_name: Dai full_name: Watanabe, Dai last_name: Watanabe - first_name: Hitoshi full_name: Inokawa, Hitoshi last_name: Inokawa - first_name: Kouichi full_name: Hashimoto, Kouichi last_name: Hashimoto - first_name: Noboru full_name: Suzuki, Noboru last_name: Suzuki - first_name: Masanobu full_name: Kano, Masanobu last_name: Kano - first_name: Ryuichi full_name: Shigemoto, Ryuichi id: 499F3ABC-F248-11E8-B48F-1D18A9856A87 last_name: Shigemoto orcid: 0000-0001-8761-9444 - first_name: Tomoo full_name: Hirano, Tomoo last_name: Hirano - first_name: Keisuke full_name: Toyama, Keisuke last_name: Toyama - first_name: Satoshi full_name: Kaneko, Satoshi last_name: Kaneko - first_name: Mineto full_name: Yokoi, Mineto last_name: Yokoi - first_name: Koki full_name: Moriyoshi, Koki last_name: Moriyoshi - first_name: Misao full_name: Suzuki, Misao last_name: Suzuki - first_name: Kazuto full_name: Kobayashi, Kazuto last_name: Kobayashi - first_name: Toshiharu full_name: Nagatsu, Toshiharu last_name: Nagatsu - first_name: Robert full_name: Kreitman, Robert last_name: Kreitman - first_name: Ira full_name: Pastan, Ira last_name: Pastan - first_name: Shigetada full_name: Nakanishi, Shigetada last_name: Nakanishi citation: ama: Watanabe D, Inokawa H, Hashimoto K, et al. Ablation of cerebellar Golgi cells disrupts synaptic integration involving GABA inhibition and NMDA receptor activation in motor coordination. Cell. 1998;95(1):17-27. doi:10.1016/S0092-8674(00)81779-1 apa: Watanabe, D., Inokawa, H., Hashimoto, K., Suzuki, N., Kano, M., Shigemoto, R., … Nakanishi, S. (1998). Ablation of cerebellar Golgi cells disrupts synaptic integration involving GABA inhibition and NMDA receptor activation in motor coordination. Cell. Cell Press. https://doi.org/10.1016/S0092-8674(00)81779-1 chicago: Watanabe, Dai, Hitoshi Inokawa, Kouichi Hashimoto, Noboru Suzuki, Masanobu Kano, Ryuichi Shigemoto, Tomoo Hirano, et al. “Ablation of Cerebellar Golgi Cells Disrupts Synaptic Integration Involving GABA Inhibition and NMDA Receptor Activation in Motor Coordination.” Cell. Cell Press, 1998. https://doi.org/10.1016/S0092-8674(00)81779-1. ieee: D. Watanabe et al., “Ablation of cerebellar Golgi cells disrupts synaptic integration involving GABA inhibition and NMDA receptor activation in motor coordination,” Cell, vol. 95, no. 1. Cell Press, pp. 17–27, 1998. ista: Watanabe D, Inokawa H, Hashimoto K, Suzuki N, Kano M, Shigemoto R, Hirano T, Toyama K, Kaneko S, Yokoi M, Moriyoshi K, Suzuki M, Kobayashi K, Nagatsu T, Kreitman R, Pastan I, Nakanishi S. 1998. Ablation of cerebellar Golgi cells disrupts synaptic integration involving GABA inhibition and NMDA receptor activation in motor coordination. Cell. 95(1), 17–27. mla: Watanabe, Dai, et al. “Ablation of Cerebellar Golgi Cells Disrupts Synaptic Integration Involving GABA Inhibition and NMDA Receptor Activation in Motor Coordination.” Cell, vol. 95, no. 1, Cell Press, 1998, pp. 17–27, doi:10.1016/S0092-8674(00)81779-1. short: D. Watanabe, H. Inokawa, K. Hashimoto, N. Suzuki, M. Kano, R. Shigemoto, T. Hirano, K. Toyama, S. Kaneko, M. Yokoi, K. Moriyoshi, M. Suzuki, K. Kobayashi, T. Nagatsu, R. Kreitman, I. Pastan, S. Nakanishi, Cell 95 (1998) 17–27. date_created: 2018-12-11T11:58:32Z date_published: 1998-10-02T00:00:00Z date_updated: 2022-08-31T13:46:20Z day: '02' doi: 10.1016/S0092-8674(00)81779-1 extern: '1' external_id: pmid: - '9778244 ' intvolume: ' 95' issue: '1' language: - iso: eng month: '10' oa_version: None page: 17 - 27 pmid: 1 publication: Cell publication_identifier: issn: - 0092-8674 publication_status: published publisher: Cell Press publist_id: '4312' quality_controlled: '1' scopus_import: '1' status: public title: Ablation of cerebellar Golgi cells disrupts synaptic integration involving GABA inhibition and NMDA receptor activation in motor coordination type: journal_article user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17 volume: 95 year: '1998' ...