---
_id: '2579'
abstract:
- lang: eng
text: The localisation of the neurokinin 3 receptor (NK3r) in the rat gastrointestinal
tract has been studied by using a polyclonal antiserum against the C-terminal
portion (amino acids 388-452) of the rat NK3r. In the oesophagus, immunoreactivity
for the NK3r was found on smooth muscle cells of the muscularis mucosae. NK3r
immunoreactivity was not present on muscle cells of other regions. Nerve cell
bodies immunoreactive for NK3r were seen in the myenteric and submucous plexuses
of the small and large intestine, but not in the stomach or oesophagus. Immunoreactivity
was largely confined to nerve cell surfaces. The reaction product was on the cell
soma and initial parts of axons. Reactivity was not seen on nerve terminals. Immunoreactive
nerve cells had Dogiel Type II morphology. Patterns of co-localisation of NK3r
and immunoreactivity for other markers were examined in the ileum, to provide
a basis from which to deduce the functional identity of NK3r-immunoreactive nerve
cells. Most of the NK3r-immunoreactive nerve cells were also immunoreactive for
the calcium-binding proteins, calretinin and calbindin, and all were immunoreactive
for the NK1 receptor (NK1r). Nerve cells that were immunoreactive for nitric oxide
synthase were not immunoreactive for either NK3r or NK1r. The projections of the
calbindin and calretinin neurons were determined by nerve lesion studies. Their
morphology, projections to the mucosa and other ganglia and immunoreactivity for
the calcium-binding proteins suggest that the NK3r-immunoreactive neurons are
intrinsic sensory neurons.
acknowledgement: This work was supported by grants from the National Health and Medical
Research Council of Australia and the National Science Foundation of the Peoples
Republic of China. Patricia Mann is a holder of a Gastroenterological Society of
Australia Post-Graduate Scholarship.
article_processing_charge: No
article_type: original
author:
- first_name: Patricia
full_name: Mann, Patricia
last_name: Mann
- first_name: Bridget
full_name: Southwell, Bridget
last_name: Southwell
- first_name: Yu
full_name: Ding, Yu
last_name: Ding
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
- first_name: John
full_name: Furness, John
last_name: Furness
citation:
ama: Mann P, Southwell B, Ding Y, Shigemoto R, Mizuno N, Furness J. Localisation
of neurokinin 3 (NK3) receptor immunoreactivity in the rat gastrointestinal tract.
Cell and Tissue Research. 1997;289(1):1-9. doi:10.1007/s004410050846
apa: Mann, P., Southwell, B., Ding, Y., Shigemoto, R., Mizuno, N., & Furness,
J. (1997). Localisation of neurokinin 3 (NK3) receptor immunoreactivity in the
rat gastrointestinal tract. Cell and Tissue Research. Springer. https://doi.org/10.1007/s004410050846
chicago: Mann, Patricia, Bridget Southwell, Yu Ding, Ryuichi Shigemoto, Noboru Mizuno,
and John Furness. “Localisation of Neurokinin 3 (NK3) Receptor Immunoreactivity
in the Rat Gastrointestinal Tract.” Cell and Tissue Research. Springer,
1997. https://doi.org/10.1007/s004410050846.
ieee: P. Mann, B. Southwell, Y. Ding, R. Shigemoto, N. Mizuno, and J. Furness, “Localisation
of neurokinin 3 (NK3) receptor immunoreactivity in the rat gastrointestinal tract,”
Cell and Tissue Research, vol. 289, no. 1. Springer, pp. 1–9, 1997.
ista: Mann P, Southwell B, Ding Y, Shigemoto R, Mizuno N, Furness J. 1997. Localisation
of neurokinin 3 (NK3) receptor immunoreactivity in the rat gastrointestinal tract.
Cell and Tissue Research. 289(1), 1–9.
mla: Mann, Patricia, et al. “Localisation of Neurokinin 3 (NK3) Receptor Immunoreactivity
in the Rat Gastrointestinal Tract.” Cell and Tissue Research, vol. 289,
no. 1, Springer, 1997, pp. 1–9, doi:10.1007/s004410050846.
short: P. Mann, B. Southwell, Y. Ding, R. Shigemoto, N. Mizuno, J. Furness, Cell
and Tissue Research 289 (1997) 1–9.
date_created: 2018-12-11T11:58:29Z
date_published: 1997-06-01T00:00:00Z
date_updated: 2022-08-22T12:19:10Z
day: '01'
doi: 10.1007/s004410050846
extern: '1'
external_id:
pmid:
- '9182595'
intvolume: ' 289'
issue: '1'
language:
- iso: eng
month: '06'
oa_version: None
page: 1 - 9
pmid: 1
publication: Cell and Tissue Research
publication_identifier:
issn:
- 0044-3794
publication_status: published
publisher: Springer
publist_id: '4319'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Localisation of neurokinin 3 (NK3) receptor immunoreactivity in the rat gastrointestinal
tract
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 289
year: '1997'
...