---
_id: '2572'
abstract:
- lang: eng
text: The distribution of the mRNA for a pituitary adenylate cyclase- activating
polypeptide (PACAP) receptor (PACAP-R) was examined in the rat brain, and also
in the hypophysis and pineal gland, by in situ hybridization with a specific 35S-labeled
riboprobe which was generated from a rat PACAP-R cDNA clone. In the brain, expression
of PACAP-R mRNA was most prominent in the periglomerular and granule cells of
the olfactory bulb, granule cells of the dentate gyrus, supraoptic nucleus, and
area postrema. The expression was also intense in the piriform, cingulate, and
retrosplenial cortices, pyramidal cells in CA2, non-pyramidal cells in CA1- CA3,
neuronal cells in the hilus of the dentate gyrus, lateral septal nucleus, intercalated
amygdaloid nucleus, anterodorsal thalamic nucleus, most of the midline and intralaminar
thalamic nuclei, many regions of the hypothalamus, dorsal motor nucleus of the
vagus nerve, hypoglossal nucleus, and lateral reticular nucleus. No significant
expression was detected in the mitral and tufted cells in the olfactory bulb,
pyramidal cells in CA1 and CA3, posterior nuclear group of the thalamus, dorsal
lateral geniculate nucleus, and Purkinje, Golgi, and granule cells in the cerebellar
cortex. Moderate-to-weak expression was further observed in many other regions
of the brain. In the cerebellar cortex, presumed Bergmann gila cells showed moderate
expression. In the hypophysis, the expression was moderate in the anterior lobe,
and weak to moderate in the posterior lobe; no significant expression was observed
in the intermediate lobe. In the pineal gland, the expression was very weak, if
any. Thus, the expression of PACAP-R was detected not only on neuronal cells but
also on some particular glial cells. The present study has shown, for the first
time, the exact site of PACAP-R expression in the brain and hypophysis. Although
the functional significance of PACAP and PACAP-R in the brain still remains to
be clarified, the present results are considered to provide some direction for
future functional studies.
acknowledgement: We are grateful for the photographic help of Mr. Akira Uesugi and
helpful discussions and support of Drs. Shige- tada Nakanishi, Yukihiko Sugimoto,
Atsushi Ichikawa, Masabumi Minami, Takeshi Ishihara, Jun Ogasawara, Daisuke Watanabe,
Akiko Tani, Yoshihiro Yoshihara, Miwa Kawasaki, Hiroshi Aino, Nobuya Ogawa, Akiko
Nishino, and Rie Hosoi. We also thank Ms. Yukiko Sakagami for secretarial assistance.
This work was supported in part by a Grant-in-Aid for Scientific Research from the
Ministry of We are grateful for the photographic help of Mr. Akira Uesugi and helpful discussions
and support of Drs. Shige- tada Nakanishi, Yukihiko Sugimoto, Atsushi Ichikawa,
Masabumi Minami, Takeshi Ishihara, Jun Ogasawara, Daisuke Watanabe, Akiko Tani,
Yoshihiro Yoshihara, Miwa Kawasaki, Hiroshi Aino, Nobuya Ogawa, Akiko Nishino,
and Rie Hosoi. We also thank Ms. Yukiko Sakagami for secretarial assistance. This
work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry
of We are grateful for the photographic help of Mr. Akira Uesugi and helpful discussions
and support of Drs. Shige- tada Nakanishi, Yukihiko Sugimoto, Atsushi Ichikawa,
Masabumi Minami, Takeshi Ishihara, Jun Ogasawara, Daisuke Watanabe, Akiko Tani,
Yoshihiro Yoshihara, Miwa Kawasaki, Hiroshi Aino, Nobuya Ogawa, Akiko Nishino,
and Rie Hosoi. We also thank Ms. Yukiko Sakagami for secretarial assistance. This
work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry
of Education, Science and Culture of Japan and by grants from Uehara Memorial Foundation
and Ono Pharmaceutical Co., Ltd
article_processing_charge: No
article_type: original
author:
- first_name: Hitoshi
full_name: Hashimoto, Hitoshi
last_name: Hashimoto
- first_name: Hiroyuki
full_name: Nogi, Hiroyuki
last_name: Nogi
- first_name: Kensaku
full_name: Mori, Kensaku
last_name: Mori
- first_name: Hitoshi
full_name: Ohishi, Hitoshi
last_name: Ohishi
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Kyohei
full_name: Yamamoto, Kyohei
last_name: Yamamoto
- first_name: Toshio
full_name: Matsuda, Toshio
last_name: Matsuda
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
- first_name: Shigekazu
full_name: Nagata, Shigekazu
last_name: Nagata
- first_name: Akemichi
full_name: Baba, Akemichi
last_name: Baba
citation:
ama: 'Hashimoto H, Nogi H, Mori K, et al. Distribution of the mRNA for a pituitary
adenylate cyclase-activating polypeptide receptor in the rat brain: An in situ
hybridization study. Journal of Comparative Neurology. 1996;371(4):567-577.
doi:10.1002/(SICI)1096-9861(19960805)371:4<567::AID-CNE6>3.3.CO;2-M'
apa: 'Hashimoto, H., Nogi, H., Mori, K., Ohishi, H., Shigemoto, R., Yamamoto, K.,
… Baba, A. (1996). Distribution of the mRNA for a pituitary adenylate cyclase-activating
polypeptide receptor in the rat brain: An in situ hybridization study. Journal
of Comparative Neurology. Wiley-Blackwell. https://doi.org/10.1002/(SICI)1096-9861(19960805)371:4<567::AID-CNE6>3.3.CO;2-M'
chicago: 'Hashimoto, Hitoshi, Hiroyuki Nogi, Kensaku Mori, Hitoshi Ohishi, Ryuichi
Shigemoto, Kyohei Yamamoto, Toshio Matsuda, Noboru Mizuno, Shigekazu Nagata, and
Akemichi Baba. “Distribution of the MRNA for a Pituitary Adenylate Cyclase-Activating
Polypeptide Receptor in the Rat Brain: An in Situ Hybridization Study.” Journal
of Comparative Neurology. Wiley-Blackwell, 1996. https://doi.org/10.1002/(SICI)1096-9861(19960805)371:4<567::AID-CNE6>3.3.CO;2-M.'
ieee: 'H. Hashimoto et al., “Distribution of the mRNA for a pituitary adenylate
cyclase-activating polypeptide receptor in the rat brain: An in situ hybridization
study,” Journal of Comparative Neurology, vol. 371, no. 4. Wiley-Blackwell,
pp. 567–577, 1996.'
ista: 'Hashimoto H, Nogi H, Mori K, Ohishi H, Shigemoto R, Yamamoto K, Matsuda T,
Mizuno N, Nagata S, Baba A. 1996. Distribution of the mRNA for a pituitary adenylate
cyclase-activating polypeptide receptor in the rat brain: An in situ hybridization
study. Journal of Comparative Neurology. 371(4), 567–577.'
mla: 'Hashimoto, Hitoshi, et al. “Distribution of the MRNA for a Pituitary Adenylate
Cyclase-Activating Polypeptide Receptor in the Rat Brain: An in Situ Hybridization
Study.” Journal of Comparative Neurology, vol. 371, no. 4, Wiley-Blackwell,
1996, pp. 567–77, doi:10.1002/(SICI)1096-9861(19960805)371:4<567::AID-CNE6>3.3.CO;2-M.'
short: H. Hashimoto, H. Nogi, K. Mori, H. Ohishi, R. Shigemoto, K. Yamamoto, T.
Matsuda, N. Mizuno, S. Nagata, A. Baba, Journal of Comparative Neurology 371 (1996)
567–577.
date_created: 2018-12-11T11:58:27Z
date_published: 1996-08-05T00:00:00Z
date_updated: 2022-08-11T13:20:31Z
day: '05'
doi: 10.1002/(SICI)1096-9861(19960805)371:4<567::AID-CNE6>3.3.CO;2-M
extern: '1'
external_id:
pmid:
- '8841910'
intvolume: ' 371'
issue: '4'
language:
- iso: eng
month: '08'
oa_version: None
page: 567 - 577
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4326'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Distribution of the mRNA for a pituitary adenylate cyclase-activating polypeptide
receptor in the rat brain: An in situ hybridization study'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 371
year: '1996'
...