Immunohistochemical localization of substance P receptor in the central nervous system of the adult rat

Nakaya Y, Kaneko T, Shigemoto R, Nakanishi S, Mizuno N. 1994. Immunohistochemical localization of substance P receptor in the central nervous system of the adult rat. Journal of Comparative Neurology. 347(2), 249–274.

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Journal Article | Published
Author
Nakaya, Yoshifumi; Kaneko, Takeshi; Shigemoto, RyuichiIST Austria ; Nakanishi, Shigetada; Mizuno, Noboru
Abstract
In an attempt to reveal the function sites of substance P (SP) in the central nervous system (CNS), the distribution of SP receptor (SPR) was immunocytochemically investigated in adult rat and compared with that of SP- positive fibers. SPR-like immunoreactivity (LI) was mostly localized to neuronal cell bodies and dendrites. Neurons with intense SPR-LI were distributed densely in the cortical amygdaloid nucleus, hilus of the dentate gyrus, locus ceruleus, rostral half of the ambiguus nucleus, and intermediolateral nucleus of the thoracic cord; moderately in the caudatoputamen, nucleus accumbens, olfactory tubercle, median, pontine, and magnus raphe nuclei, laminae I and III of the caudal subnucleus of the spinal trigeminal nucleus, and lamina I of the spinal cord; and sparsely in the cerebral cortex, basal nucleus of Meynert, claustrum, gigantocellular reticular nucleus, and lobules IX and X of the cerebellar vermis. Neurons with weak to moderate SPR-LI were distributed more widely throughout the CNS. The regional patterns of distribution of SPR-LI were not necessarily the same as those of SP-positive fibers. The entopeduncular nucleus, substantia nigra, and lateral part of the interpeduncular nucleus showed intense SP-LI but displayed almost no SPR-LI. Conversely, the hilus of the dentate gyrus, anterodorsal thalamic nucleus, central nucleus of the inferior colliculus, and dorsal tegmental nucleus showed intense to moderate SPR-LI but contained few axons with SP-LI. These findings confirmed the presence of the 'mismatch' problem between SP and SPR localizations. However, the distribution of SPR- LI was quite consistent with that of the SP-binding activity, which has been studied via autoradiography. This indicates that the sites of SPR-LI revealed in the present study represent most, if not all, sites of SP-binding activity.
Publishing Year
Date Published
1994-09-08
Journal Title
Journal of Comparative Neurology
Volume
347
Issue
2
Page
249 - 274
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Nakaya Y, Kaneko T, Shigemoto R, Nakanishi S, Mizuno N. Immunohistochemical localization of substance P receptor in the central nervous system of the adult rat. Journal of Comparative Neurology. 1994;347(2):249-274. doi:10.1002/cne.903470208
Nakaya, Y., Kaneko, T., Shigemoto, R., Nakanishi, S., & Mizuno, N. (1994). Immunohistochemical localization of substance P receptor in the central nervous system of the adult rat. Journal of Comparative Neurology. Wiley-Blackwell. https://doi.org/10.1002/cne.903470208
Nakaya, Yoshifumi, Takeshi Kaneko, Ryuichi Shigemoto, Shigetada Nakanishi, and Noboru Mizuno. “Immunohistochemical Localization of Substance P Receptor in the Central Nervous System of the Adult Rat.” Journal of Comparative Neurology. Wiley-Blackwell, 1994. https://doi.org/10.1002/cne.903470208.
Y. Nakaya, T. Kaneko, R. Shigemoto, S. Nakanishi, and N. Mizuno, “Immunohistochemical localization of substance P receptor in the central nervous system of the adult rat,” Journal of Comparative Neurology, vol. 347, no. 2. Wiley-Blackwell, pp. 249–274, 1994.
Nakaya Y, Kaneko T, Shigemoto R, Nakanishi S, Mizuno N. 1994. Immunohistochemical localization of substance P receptor in the central nervous system of the adult rat. Journal of Comparative Neurology. 347(2), 249–274.
Nakaya, Yoshifumi, et al. “Immunohistochemical Localization of Substance P Receptor in the Central Nervous System of the Adult Rat.” Journal of Comparative Neurology, vol. 347, no. 2, Wiley-Blackwell, 1994, pp. 249–74, doi:10.1002/cne.903470208.

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