--- _id: '2537' abstract: - lang: eng text: 'The metabotropic glutamate receptors are coupled to intracellular signal transduction via G-proteins and consist of a family of at least five different subtypes, termed mGluR1-mGluR5. We studied the signal transduction mechanism and pharmacological characteristics of the rat mGluR3 and mGluR4 subtypes in Chinese hamster ovary cells permanently expressing the cloned receptors. Both mGluR3 and mGluR4 inhibit the forskolin-stimulated accumulation of intracellular cAMP formation in response to agonist interaction. Consistent with the high degree of sequence similarity to mGluR2, mGluR3 closely resembles mGluR2 in its agonist selectivity; the potency rank order of agonists is L-glutamate > trans-1-aminocyclopentane- 1,3-dicarboxylate > ibotenate > quisqualate. mGluR4 is totally different in its agonist specificity from any other member of the metabotropic receptors. This receptor potently reacts with L-2-amino-4-phosphonobutyrate(L-AP4) in a stereo-selective manner and moderately responds to L-serine-O-phosphate. mGluR4 thus corresponds well to the putative L-AP4 receptor characterized from brain preparations. Blot and in situ hybridization analyses indicated that both mRNAs are widely distributed in the rat brain. mGluR3 mRNA is highly expressed in neuronal cells of the cerebral cortex and the caudate- putamen, and in granule cells of the hippocampal dentate gyrus. The expression pattern of mGluR4 mRNA is more restricted, and this expression is prominent in the cerebellum, olfactory bulb, and thalamus. Furthermore, the mGluR3 mRNA, unlike the other mRNAs for the metabotropic receptors, is highly expressed in glial cells throughout the brain regions. The metabotropic glutamate receptor subtypes can thus be classified into three subgroups according to the similarity in their amino acid sequences, signal transduction, and agonist selectivity: mGluR1/mGluR5, mGluR2/mGluR3, and mGluR4. The mRNAs for the individual receptor subtypes, however, show overlapping but distinct patterns of expression in the rat CNS.' acknowledgement: 'We are grateful to Mr. Akira Uesugi for photographic assistance. This work was supported in part by research grants from the Ministry of Education, Science and Culture of Japan, the Ministry of Health and Welfare of Japan, the Uehara Memorial Foundation, and the Semi Life Science Foundation. ' article_processing_charge: No article_type: original author: - first_name: Yasuto full_name: Tanabe, Yasuto last_name: Tanabe - first_name: Akinori full_name: Nomura, Akinori last_name: Nomura - first_name: Masayuki full_name: Masu, Masayuki last_name: Masu - first_name: Ryuichi full_name: Shigemoto, Ryuichi id: 499F3ABC-F248-11E8-B48F-1D18A9856A87 last_name: Shigemoto orcid: 0000-0001-8761-9444 - first_name: Noboru full_name: Mizuno, Noboru last_name: Mizuno - first_name: Shigetada full_name: Nakanishi, Shigetada last_name: Nakanishi citation: ama: Tanabe Y, Nomura A, Masu M, Shigemoto R, Mizuno N, Nakanishi S. Signal transduction, pharmacological properties, and expression patterns of two rat metabotropic glutamate receptors, mGluR3 and mGluR4. Journal of Neuroscience. 1993;13(4):1372-1378. doi:10.1523/JNEUROSCI.13-04-01372.1993 apa: Tanabe, Y., Nomura, A., Masu, M., Shigemoto, R., Mizuno, N., & Nakanishi, S. (1993). Signal transduction, pharmacological properties, and expression patterns of two rat metabotropic glutamate receptors, mGluR3 and mGluR4. Journal of Neuroscience. Society for Neuroscience. https://doi.org/10.1523/JNEUROSCI.13-04-01372.1993 chicago: Tanabe, Yasuto, Akinori Nomura, Masayuki Masu, Ryuichi Shigemoto, Noboru Mizuno, and Shigetada Nakanishi. “Signal Transduction, Pharmacological Properties, and Expression Patterns of Two Rat Metabotropic Glutamate Receptors, MGluR3 and MGluR4.” Journal of Neuroscience. Society for Neuroscience, 1993. https://doi.org/10.1523/JNEUROSCI.13-04-01372.1993. ieee: Y. Tanabe, A. Nomura, M. Masu, R. Shigemoto, N. Mizuno, and S. Nakanishi, “Signal transduction, pharmacological properties, and expression patterns of two rat metabotropic glutamate receptors, mGluR3 and mGluR4,” Journal of Neuroscience, vol. 13, no. 4. Society for Neuroscience, pp. 1372–1378, 1993. ista: Tanabe Y, Nomura A, Masu M, Shigemoto R, Mizuno N, Nakanishi S. 1993. Signal transduction, pharmacological properties, and expression patterns of two rat metabotropic glutamate receptors, mGluR3 and mGluR4. Journal of Neuroscience. 13(4), 1372–1378. mla: Tanabe, Yasuto, et al. “Signal Transduction, Pharmacological Properties, and Expression Patterns of Two Rat Metabotropic Glutamate Receptors, MGluR3 and MGluR4.” Journal of Neuroscience, vol. 13, no. 4, Society for Neuroscience, 1993, pp. 1372–78, doi:10.1523/JNEUROSCI.13-04-01372.1993. short: Y. Tanabe, A. Nomura, M. Masu, R. Shigemoto, N. Mizuno, S. Nakanishi, Journal of Neuroscience 13 (1993) 1372–1378. date_created: 2018-12-11T11:58:15Z date_published: 1993-04-01T00:00:00Z date_updated: 2022-03-31T14:49:42Z day: '01' doi: 10.1523/JNEUROSCI.13-04-01372.1993 extern: '1' external_id: pmid: - '8463825' intvolume: ' 13' issue: '4' language: - iso: eng main_file_link: - open_access: '1' url: https://pubmed.ncbi.nlm.nih.gov/8463825/ month: '04' oa: 1 oa_version: Published Version page: 1372 - 1378 pmid: 1 publication: Journal of Neuroscience publication_identifier: issn: - 0270-6474 publication_status: published publisher: Society for Neuroscience publist_id: '4361' quality_controlled: '1' scopus_import: '1' status: public title: Signal transduction, pharmacological properties, and expression patterns of two rat metabotropic glutamate receptors, mGluR3 and mGluR4 type: journal_article user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17 volume: 13 year: '1993' ...