--- _id: '2534' abstract: - lang: eng text: Vasoactive intestinal polypeptide (VIP), a 28 amino acid peptide hormone, plays many physiological roles in the peripheral and central nerve systems. A functional cDNA clone of the VIP receptor was isolated from a rat lung cDNA library by cross-hybridization with the secretin receptor cDNA. VIP bound the cloned VIP receptor expressed in mouse COP cells and stimulated adenylate cyclase through the cloned receptor. The rat VIP receptor consists of 459 amino acids with a calculated Mr of 52,054 and contains seven transmembrane segments. It is structurally related to the secretin, calcitonin, and parathyroid hormone receptors, suggesting that they constitute a new subfamily of the G5 protein - coupled receptors. VIP receptor mRNA was detected in various rat tissues including liver, lung, intestines, and brain. In situ hybridization revealed that VIP receptor mRNA is widely distributed in neuronal cells of the adult rat brain, with a relatively high expression in the cerebral cortex and hippocampus. acknowledgement: "We thank Drs. R. Yoshida, K. Katoh, and K. lmamura for help with the in situ hybridization, Dr. M. Nishizawa for discussion, and Ms. M. lkeda for secretarial assistance. This work was supported in part by a Grant-in-Aid from the Ministry of Education, Science and Culture of Japan. The costs of publication of this article were defrayed in part\r\nby the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 USC Section 1734 solely to indicate this fact." article_processing_charge: No article_type: original author: - first_name: Takeshi full_name: Ishihara, Takeshi last_name: Ishihara - first_name: Ryuichi full_name: Shigemoto, Ryuichi id: 499F3ABC-F248-11E8-B48F-1D18A9856A87 last_name: Shigemoto orcid: 0000-0001-8761-9444 - first_name: Kensaku full_name: Mori, Kensaku last_name: Mori - first_name: Kenji full_name: Takahashi, Kenji last_name: Takahashi - first_name: Shigekazu full_name: Nagata, Shigekazu last_name: Nagata citation: ama: Ishihara T, Shigemoto R, Mori K, Takahashi K, Nagata S. Functional expression and tissue distribution of a novel receptor for vasoactive intestinal polypeptide. Neuron. 1992;8(4):811-819. doi:10.1016/0896-6273(92)90101-I apa: Ishihara, T., Shigemoto, R., Mori, K., Takahashi, K., & Nagata, S. (1992). Functional expression and tissue distribution of a novel receptor for vasoactive intestinal polypeptide. Neuron. Elsevier. https://doi.org/10.1016/0896-6273(92)90101-I chicago: Ishihara, Takeshi, Ryuichi Shigemoto, Kensaku Mori, Kenji Takahashi, and Shigekazu Nagata. “Functional Expression and Tissue Distribution of a Novel Receptor for Vasoactive Intestinal Polypeptide.” Neuron. Elsevier, 1992. https://doi.org/10.1016/0896-6273(92)90101-I. ieee: T. Ishihara, R. Shigemoto, K. Mori, K. Takahashi, and S. Nagata, “Functional expression and tissue distribution of a novel receptor for vasoactive intestinal polypeptide,” Neuron, vol. 8, no. 4. Elsevier, pp. 811–819, 1992. ista: Ishihara T, Shigemoto R, Mori K, Takahashi K, Nagata S. 1992. Functional expression and tissue distribution of a novel receptor for vasoactive intestinal polypeptide. Neuron. 8(4), 811–819. mla: Ishihara, Takeshi, et al. “Functional Expression and Tissue Distribution of a Novel Receptor for Vasoactive Intestinal Polypeptide.” Neuron, vol. 8, no. 4, Elsevier, 1992, pp. 811–19, doi:10.1016/0896-6273(92)90101-I. short: T. Ishihara, R. Shigemoto, K. Mori, K. Takahashi, S. Nagata, Neuron 8 (1992) 811–819. date_created: 2018-12-11T11:58:14Z date_published: 1992-04-01T00:00:00Z date_updated: 2022-03-17T13:33:07Z day: '01' doi: 10.1016/0896-6273(92)90101-I extern: '1' external_id: pmid: - '1314625' intvolume: ' 8' issue: '4' language: - iso: eng main_file_link: - url: https://www.sciencedirect.com/science/article/pii/089662739290101I?via%3Dihub month: '04' oa_version: None page: 811 - 819 pmid: 1 publication: Neuron publication_identifier: issn: - 0896-6273 publication_status: published publisher: Elsevier publist_id: '4363' quality_controlled: '1' scopus_import: '1' status: public title: Functional expression and tissue distribution of a novel receptor for vasoactive intestinal polypeptide type: journal_article user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17 volume: 8 year: '1992' ...