--- _id: '2533' abstract: - lang: eng text: A cDNA clone for a new metabotropic glutamate receptor, mGluR5, was isolated through polymerase chain reaction-mediated DNA amplification by using primer sequences conserved among the metabotropic glutamate receptor (mGluR) family and by the subsequent screening of a rat brain cDNA library. The cloned receptor consists of 1171 amino acid residues and exhibits a structural architecture common to the mGluR family, possessing a large extracellular domain preceding the seven putative membrane-spanning segments. mGluR5 shows the highest sequence similarity to mGluR1 among the mGluR members and is coupled to the stimulation of phosphatidylinositol hydrolysis/ Ca2+ signal transduction in Chinese hamster ovary cells transfected with the cloned cDNA. This receptor also resembles mGluR1 in its agonist selectivity and antagonist responses; the potency rank order of agonists for mGluR5 was determined to be quisqualate > L-glutamate ≥ ibotenate > trans-1-aminocyclopentane-1,3-dicarboxylate. Blot and in situ hybridization analyses indicated that mGluR5 mRNA is widely distributed in neuronal cells of the central nervous system and is expressed differently from mGluR1 mRNA in many brain regions. This investigation thus demonstrates that there is an additional mGluR subtype which closely resembles mGluR1 in its signal transduction and pharmacological properties and is expressed in specialized neuronal cells in the central nervous system. acknowledgement: We are grateful to Seiji Ito for help of Ca2+ measurements and Akira Uesugi for photographic assistance. article_processing_charge: No article_type: original author: - first_name: Takaaki full_name: Abe, Takaaki last_name: Abe - first_name: Hidemitsu full_name: Sugihara, Hidemitsu last_name: Sugihara - first_name: Hiroyuki full_name: Nawa, Hiroyuki last_name: Nawa - first_name: Ryuichi full_name: Shigemoto, Ryuichi id: 499F3ABC-F248-11E8-B48F-1D18A9856A87 last_name: Shigemoto orcid: 0000-0001-8761-9444 - first_name: Noboru full_name: Mizuno, Noboru last_name: Mizuno - first_name: Shigetada full_name: Nakanishi, Shigetada last_name: Nakanishi citation: ama: Abe T, Sugihara H, Nawa H, Shigemoto R, Mizuno N, Nakanishi S. Molecular characterization of a novel metabotropic glutamate receptor mGluR5 coupled to inositol phosphate/Ca2+ signal transduction. Journal of Biological Chemistry. 1992;267(19):13361-13368. doi:10.1016/S0021-9258(18)42219-3 apa: Abe, T., Sugihara, H., Nawa, H., Shigemoto, R., Mizuno, N., & Nakanishi, S. (1992). Molecular characterization of a novel metabotropic glutamate receptor mGluR5 coupled to inositol phosphate/Ca2+ signal transduction. Journal of Biological Chemistry. American Society for Biochemistry and Molecular Biology. https://doi.org/10.1016/S0021-9258(18)42219-3 chicago: Abe, Takaaki, Hidemitsu Sugihara, Hiroyuki Nawa, Ryuichi Shigemoto, Noboru Mizuno, and Shigetada Nakanishi. “Molecular Characterization of a Novel Metabotropic Glutamate Receptor MGluR5 Coupled to Inositol Phosphate/Ca2+ Signal Transduction.” Journal of Biological Chemistry. American Society for Biochemistry and Molecular Biology, 1992. https://doi.org/10.1016/S0021-9258(18)42219-3. ieee: T. Abe, H. Sugihara, H. Nawa, R. Shigemoto, N. Mizuno, and S. Nakanishi, “Molecular characterization of a novel metabotropic glutamate receptor mGluR5 coupled to inositol phosphate/Ca2+ signal transduction,” Journal of Biological Chemistry, vol. 267, no. 19. American Society for Biochemistry and Molecular Biology, pp. 13361–13368, 1992. ista: Abe T, Sugihara H, Nawa H, Shigemoto R, Mizuno N, Nakanishi S. 1992. Molecular characterization of a novel metabotropic glutamate receptor mGluR5 coupled to inositol phosphate/Ca2+ signal transduction. Journal of Biological Chemistry. 267(19), 13361–13368. mla: Abe, Takaaki, et al. “Molecular Characterization of a Novel Metabotropic Glutamate Receptor MGluR5 Coupled to Inositol Phosphate/Ca2+ Signal Transduction.” Journal of Biological Chemistry, vol. 267, no. 19, American Society for Biochemistry and Molecular Biology, 1992, pp. 13361–68, doi:10.1016/S0021-9258(18)42219-3. short: T. Abe, H. Sugihara, H. Nawa, R. Shigemoto, N. Mizuno, S. Nakanishi, Journal of Biological Chemistry 267 (1992) 13361–13368. date_created: 2018-12-11T11:58:14Z date_published: 1992-07-05T00:00:00Z date_updated: 2022-03-17T15:08:29Z day: '05' doi: 10.1016/S0021-9258(18)42219-3 extern: '1' external_id: pmid: - '1320017' intvolume: ' 267' issue: '19' language: - iso: eng main_file_link: - open_access: '1' url: https://www.sciencedirect.com/science/article/pii/S0021925818422193 month: '07' oa: 1 oa_version: Published Version page: 13361 - 13368 pmid: 1 publication: Journal of Biological Chemistry publication_identifier: issn: - 0021-9258 publication_status: published publisher: American Society for Biochemistry and Molecular Biology publist_id: '4366' quality_controlled: '1' scopus_import: '1' status: public title: Molecular characterization of a novel metabotropic glutamate receptor mGluR5 coupled to inositol phosphate/Ca2+ signal transduction type: journal_article user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17 volume: 267 year: '1992' ...